镰刀菌毒素对断奶仔猪血清酶、肝脏抗氧化功能及组织病理学的影响

doi:10.16431/j.cnki.1671-7236.2015.09.026

摘要/Abstract

摘要： 试验旨在研究镰刀菌毒素污染日粮对断奶仔猪血清酶、肝脏抗氧化功能及组织病理学的影响。选择35日龄体重为(8.45±0.94) kg的健康三元杂交(杜×长×大)断奶雌性仔猪40头,随机分为2组,每组20个重复,每个重复1头猪。对照组饲喂基础日粮,试验组饲喂用霉变玉米和霉变玉米蛋白粉替代基础日粮中50%的玉米和玉米蛋白粉配制而成的镰刀菌毒素污染日粮(玉米赤霉烯酮(ZEN) 0.90 mg/kg,呕吐毒素(DON) 1.43 mg/kg,烟曲霉毒素(FUM) 5.85 mg/kg)。仔猪单栏饲喂,预试期7 d,正试期35 d。结果表明:①与对照组相比,试验组断奶仔猪血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)和碱性磷酸酶(ALP)活性均显著升高(P<0.05),肝脏相对重显著降低(P<0.05);②与对照组相比,试验组断奶仔猪血清和肝脏中谷胱甘肽过氧化物酶(GSH-Px)和总超氧化物歧化酶(T-SOD)活性均显著降低(P<0.05),而丙二醛(MDA)含量显著升高(P<0.05);③病理组织学观察发现,试验组断奶仔猪肝脏出现一定程度的组织损伤,部分肝小叶边缘发生明显的炎性细胞浸润,肝细胞发生局部空泡变性。由此可知,镰刀菌毒素污染日粮(0.90 mg/kg ZEN,1.43 mg/kg DON,5.85 mg/kg FUM)饲喂断奶仔猪可引起仔猪血清酶活性改变,肝脏抗氧化功能降低,肝脏组织出现一定程度的病理损伤,从而影响肝脏功能的发挥。

关键词: 镰刀菌毒素; 血清酶; 肝脏; 抗氧化功能; 组织病理学; 断奶仔猪

Abstract: The objective of the present study was to investigate the effects of Fusarium toxins contaminated diet on serum enzymes, liver antioxidant function and histopathology of weaned gilts.Forty healthy weaned gilts (Duroc×Landrace×Yorkshire) aged 35 d with body weight of (8.45±0.94) kg were randomly allocated into 2 groups with 20 replicates per group and 1 pigs per replicate.Gilts in the control group were fed with basal diet, and those in the experiment group were fed with Fusarium toxins contaminated diet made by replacing 50% of corn and corn gluten meal in the basal diet with the naturally contaminated corn and corn gluten meal (0.90 mg/kg ZEN, 1.43 mg/kg DON, 5.85 mg/kg FUM).Gilts were fed individually in a metabolic cage for 35 d after 7 d adaptation.The results showed as follows:① Compared with control group, the serum activities of AST, ALT and ALP in experimental group were significantly increased (P<0.05), and the liver index of experiment group was significantly decreased (P<0.05).②Compared with control group, the activities of GSH-Px and T-SOD in serum and liver of experimental group were significantly decreased (P<0.05), and the MDA content in serum and liver were significantly increased (P<0.05).③ Histological examination of liver in experiment group revealed a certain degree of damage, with obvious inflammatory cell infiltration partly at the margin of hepatic lobule and vacuoles degeneration of local hepatic cells.The results indicated that Fusarium toxins contaminated diets (0.90 mg/kg ZEN, 1.43 mg/kg DON, 5.85 mg/kg FUM) could change serum enzymes activity and impair liver antioxidant function, with a certain degree of pathological injury of liver in the present study, which had a negative impact on the normal liver function in weaned gilts.

Key words: Fusarium toxins; serum enzymes; liver; antioxidant function; histopathology; weaned gilts

中图分类号:

S828

陈宁波, 张崇玉, 姜淑贞, 黄丽波, 陈祥兴, 孙建立, 杨维仁. 镰刀菌毒素对断奶仔猪血清酶、肝脏抗氧化功能及组织病理学的影响[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(9): 2384-2390.

CHEN Ning-bo, ZHANG Chong-yu, JIANG Shu-zhen, Huang Li-bo, CHEN Xiang-xing, SUN Jian-li, YANG Wei-ren. Effects of Fusarium Toxins on Serum Enzymes Activity,Liver Antioxidant Function and Histopathology of Weaned Gilts[J]. , 2015, 42(9): 2384-2390.

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