同日龄不同体重羔羊十二指肠形态功能的比较研究

doi:10.16431/j.cnki.1671-7236.2015.08.024

摘要/Abstract

摘要： 为了解小肠消化吸收功能对羔羊生长发育的影响,本试验选择60只相同饲养条件、相同断奶日龄(3月龄断奶)的5月龄健康阿勒泰×特克赛尔杂交F1代羔羊为研究对象,从中选择体重大小具有显著性差异的8只羔羊分为大体重组(HBW组)4只,平均体重(37.20±2.80)kg;小体重组(LBW组)4只,平均体重(28.24±3.75)kg。测定两组羔羊十二指肠绒毛组织形态学指标、消化酶活性及其黏膜中钠依赖性葡萄糖转载蛋白1(SGLT1)和葡萄糖转载蛋白2(GLUT2)表达量的差异。结果显示,HBW组羔羊十二指肠绒毛高度、隐窝深度均显著低于LBW组(P< 0.05),而黏膜厚度、绒毛高度/隐窝深度比值差异均不显著(P> 0.05);HBW组羔羊淀粉酶活性显著高于LBW组羔羊(P< 0.05),麦芽糖酶、乳糖酶、蔗糖酶活性均极显著高于LBW组羔羊(P< 0.01),脂肪酶及蛋白酶活性在两组羔羊间均无显著差异(P> 0.05);HBW组羔羊SGLT1表达量显著高于LBW组(P< 0.05),GLUT2表达量极显著高于LBW组(P< 0.01)。综上所述,反刍动物体重增加与十二指肠消化吸收功能密切相关,这些消化酶及葡萄糖运载蛋白的分子生物学上调机制尚需进一步研究。

关键词: 羔羊; 生长速度; 小肠黏膜; 淀粉酶; 蔗糖酶; 葡萄糖转载蛋白

Abstract: To understand the effect of intestinal digestion and absorption on the growth and development of lambs.60 5-month and healthy lambs were selected as the study subjects which were F1 generation hybridized by Altay sheep × Teksaier sheep and were fed in the same rearing conditions and had the same weaning date.And then, 8 lambs with significant weight differences were equally divided into HBW group and LBW group, the former with average weight (37.20±2.80)kg and the latter with average weight (28.24±3.75)kg.Measurements were to be performed for the differences in the morphological indicators of villus in duodenum, digestive enzyme activity in duodenum and sodium glucose cotransporter 1 (SGLT1) and glucose transporter 2 (GLUT2) in the lambs'mucosa.The results showed that the villus height and crypt depth of duodenum in HBW group were significantly lower than that in LBW group (P< 0.05), there were no significant differences in the mucosal thickness and V/C between two groups (P> 0.05);The amylase activity in HBW group was significantly higher than that in LBW group (P< 0.05), the activities of maltase, lactase and sucrase in HBW group were extremely significantly higher than that in LBW group (P< 0.01), there were no significant differences in the activities of lipase and protease between two groups (P> 0.05);The expression amount of SGLT1 in HBW group was significantly higher than that in LBW group (P< 0.05), the expression amount of GLUT2 in HBW group was extremely significantly higher than that in LBW group (P< 0.01).In conclusion, there was close correlation between the weight increment of the lambs and digestive and absorptive functions of duodenum, and that further research and study need to be conducted to have a better understanding of the regulating mechanism of molecular biology for digestive enzyme and glucose transporter.

Key words: lamb; growth rate; small intestinal mucosa; amylase; sucrase; glucose transfer protein

中图分类号:

S826

马雪连, 王金泉, 张晓红, 姚刚. 同日龄不同体重羔羊十二指肠形态功能的比较研究[J]. , 2015, 42(8): 2091-2097.

MA Xue-lian, WANG Jin-quan, ZHANG Xiao-hong, YAO Gang. Study on the Morphology and Fuction of the Duodenum in Lambs with Same Weaning Age but Different Body Weight[J]. , 2015, 42(8): 2091-2097.

参考文献

[1] Titi H H,Tabbaa M J,Amasheh M G,et al.Comparative performance of Awassi lambs and Black goat kids on different crude protein levels in Jordan[J].Small Rumin Res,2000,37(1-2):131-135.
[2] 陈前岭,王梦芝,喻礼怀,等.生长期不同品种牛饲喂颗粒化TMR日粮生长性能的比较[J].饲料工业,2013,34(24):37-41.
[3] Lebel A,Matte J J,Guay F.Effect of mineral source and mannan oligosaccharide supplements on zinc and copper digestibility in growing pigs[J].Arch Anim Nutr,2014,68(5):370-384.
[4] Hill T M,Bateman H G,Aldrich J M,et al.Effects of fat concentration of a high-protein milk replacer on calf performance[J].J Dairy Sci,2009,92(10):5147-5153.
[5] 王定发,周璐丽,李茂,等.不同营养水平日粮对海南黑山羊育肥羔羊生长性能和器官指数的影响[J].中国畜牧兽医,2013,40(2):62-65.
[6] 孙爽,罗军,李芳,等.不同饲喂方案对西农萨能山羊羔羊生长发育的影响[J].中国草食动物,2012,32:39-42.
[7] 王永军,陈玉林,赵永贞,等.小尾寒羊羔羊隔栏补饲效果研究[J].家畜生态学,2003,8:14-16.
[8] Ali C S,Din I,Sharif M,等.过瘤胃蛋白和氨基酸对奶牛、绵羊干物质采食量、消化率和生长性能的影响[J].饲料与畜牧,2011,3:44-48.
[9] 吕秋凤,董欣,邵彩梅,等.非淀粉多糖酶的作用机理及在畜禽生产中应用的研究进展[J].中国畜牧杂志,2014,11:90-94.
[10] Belanche A,Doreau M,Edwards J E,et al.Shifts in the rumen microbiota due to the type of carbohydrate and level of protein ingested by dairy cattle are associated with changes in rumen fermentation[J].J Nutr,2012,142(9):1684-1692.
[11] Zhao X H,Zhang T,Xu M,et al.Effects of physically effective fiber on chewing activity,ruminal fermentation,and digestibility in goats[J].Journal of Animal Science,2011,89(2):501-509.
[12] 朱文涛.15~150日龄羔羊日粮消化率和瘤胃消化功能变化的研究[D].乌鲁木齐:新疆农业大学,2005.
[13] 任成林,田勇,梁淑珍.动物组织H.E.染色石蜡切片技术的改进[J].河北北方学院学报(自然科学版),2007,23(1):41-45.
[14] 姚浪群,萨仁娜,佟建明,等.安普霉素对仔猪肠道微生物及肠壁组织结构的影响[J].畜牧兽医学报,2003,34(3):250-257.
[15] 王子旭,佘锐萍,陈越,等.日粮锌硒水平对肉鸡小肠黏膜结构的影响[J].中国兽医科技,2003,33(7):18-21.
[16] 韩正康.家畜营养生理学[M].北京:中国农业出版社,1991.
[17] 陈瑞芳,郭傲民,耿丹,等.水牛初乳粉和常乳粉对新生仔猪小肠组织形态结构的影响[J].中国畜牧兽医,2014,41(4):137-142.
[18] Stevens B R,Kaunitzn J D,Wright E M.Intestinal transport of amino acids and sugars:Advances using membrane vesicles[J].Annual Review of Physiology,1984,46(1):417-433.
[19] 王俊锋,刘美,徐凤霞,等.反刍动物葡萄糖的营养代谢研究进展[J].饲料工业,2004,25(5):7-10.
[20] 郭冬生,彭小兰,夏维福.反刍动物葡萄糖代谢与营养研究进展[J].广东农业科学,2011,8:98-99.
[21] Kellett G L,Brot-Laroche E,Mace O J,et al.Sugar absorption in the intestine:The role of GLUT2[J].Annu Rev Nutr,2008,28:35-54.
[22] Au A,Gupta A,Schembri P,et al.Rapid insertion of GLUT2 into the rat jejunal brush-border membrane promoted by glucagon-like peptide 2[J].Biochem J,2002,367:247-254.
[23] 李勇,蔡辉益,刘国华.动物肠道葡萄糖吸收调节的分子机制研究进展[J].中国畜牧兽医,2007,34(8):5-9.
[24] Dyer J,Barker P J,Shirazi-Beechey S P.Nutrient regulation of the intestinal Na⁺/glucose co-transporter (SGLT1) gene expression[J].J Biochemical and Biophysical Research Communications,1997,230(3):624-629.
[25] 张英杰,刘月琴,孙洪新,等.羔羊小肠pH及主要消化酶发育规律的研究[J].畜牧兽医学报,2005,36(2):149-152.
[26] 郎侠.欧拉型藏羊消化系统发育和肌肉H-FABP基因表达规律的研究[D].兰州:甘肃农业大学,2011.
[27] Harmon D L,McLeod K R.Glucose uptake and regulation by intestinal tissues:Implications and whole-body energetics[J].J Anim Sci,2001,79(Suppl E):E59.
[28] Bauer M L,Harmon D L,Bohnert D W,et al.Influence of alpha-linked glucose on sodium-glucose cotransport activity along the small intestine in cattle[J].J Anim Sci,2001,79(7):1917-1924.
[29] Alexander N A,Carey H V.Involvement of PI 3-kinase in IGF-Ⅰ stimulation of jejunal Na⁺-K⁺-ATPase activity and nutrient absorption[J].American Journal of Physiology-Gastrointestinal Liver Physiology,2001,280(2):G222-G228.
[30] Aschenbach J R,Wehning H,Kurze M,et al.Functional and molecular biological evidence of SGLT-1 in the ruminal epithelium of sheep[J].American Journal of Physiology-Gastrointestinal Liver Physiology,2000,279(1):G20-G27.