牛源大肠杆菌Ⅰ类整合子分子特征及其与耐药性关系的研究

中国畜牧兽医 ›› 2014, Vol. 41 ›› Issue (9): 63-68.

牛源大肠杆菌Ⅰ类整合子分子特征及其与耐药性关系的研究

王海生^1,2, 扈瑞平², 高玉敏³, 杨钟⁴, 邓秀玲², 张建宇², 武瑞兵², 呼和巴特尔¹

1. 内蒙古农业大学兽医学院, 内蒙古呼和浩特 010018;
2. 内蒙古医科大学生物化学与分子生物学教研室, 内蒙古呼和浩特 010110;
3. 内蒙古医科大学公共卫生学院, 内蒙古呼和浩特 010110;
4. 呼和浩特疾病预防控制中心, 内蒙古呼和浩特 010070

修回日期:2014-05-26 出版日期:2014-09-20 发布日期:2014-09-24
通讯作者: 武瑞兵, 呼和巴特尔 E-mail:wuruib@gmail.com;hhbte@yahoo.com.cn
作者简介:王海生（1973- ），男，内蒙古人，博士生，副教授，研究方向：细胞生长机制。
基金资助:
内蒙古自治区教育厅自然科学重点项目（NJZZ12149）；内蒙古医科大学博士启动基金项目（bsjj201405）。

Study on Molecular Characterization of Class Ⅰ Integron and Integron-associated Antimicrobial Resistance in Escherichia coli from Beef Cattle

WANG Hai-sheng^1,2, HU Rui-ping², GAO Yu-min³, YANG Zhong⁴, DENG Xiu-ling², ZHANG Jian-yu², WU Rui-bing², HUHE Bateer¹

1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Department of Biochemistry and Molecular Biology, Inner Mongolia Medical University, Hohhot 010110, China;
3. College of Publish Health, Inner Mongolia Medical University, Hohhot 010110, China;
4. Hohhot Center for Disease Control and Prevention, Hohhot 010070, China

Revised:2014-05-26 Online:2014-09-20 Published:2014-09-24

摘要/Abstract

摘要： 本试验旨在了解牛源大肠杆菌中Ⅰ类整合子的流行情况和分子特性，并分析其与细菌耐药性的关系。本研究选取92株从肉牛非重复分离的大肠杆菌，经全自动药敏分析仪对11种常用抗生素进行了药敏检测分析，并利用PCR技术和直接测序法对牛源大肠杆菌中Ⅰ类整合酶基因及Ⅰ类整合子阳性菌株中可变区基因盒进行了调查、分析及鉴定。结果显示，92株牛源大肠杆菌中有29株检测到Ⅰ类整合酶基因，检出率为31.5%；2种可变区基因盒排列最为流行，分别为aadA1和dfrA17＋aadA5；92株大肠杆菌对氨苄西林、链霉素、磺胺异恶唑和四环素的耐药率均大于45.0%，Ⅰ类整合子阳性菌株对氨苄西林、氯霉素、链霉素、磺胺异恶唑和甲氧苄啶/磺胺甲恶唑的耐药率均显著高于Ⅰ类整合子阴性菌株（P<0.05）。结果表明本试验分离的牛源大肠杆菌耐药现象非常严重，整合子/基因盒分布广泛，已成为耐药基因储库，对耐药基因传播起重要作用，提示应严格控制动物饲料中抗生素添加剂的滥用。

关键词: Ⅰ类整合子; 基因盒; 耐药性; 大肠杆菌

Abstract: To investigate the prevalence and molecular characterization of class Ⅰ integron in Escherichia coli isolated from beef cattle, and analyze the relationship between integron and antimicrobial resistance, susceptibilities to 11 antimicrobials were conducted on 92 isolates, the presence and characterization of class Ⅰ integrons and inserted gene cassettes were performed using PCR combined with sequencing analysis. The results showed that 29 isolates had been detected positive for class Ⅰ integron integrase gene (intⅠ1) among 92 isolates, and aadA1 and dfrA17＋aadA5 were the most prevalent gene cassette arrays detected. The resistance rates of 92 isolates to ampicillin, streptomycin, sulfisoxazole and tetracycline were all more than 45.0%. As revealed by analyzing the association between resistance phenotypes and class Ⅰ integron, isolates that contained the class Ⅰ integron were significantly highly resistant to ampicillin, chloramphenicol, streptomycin, sulfisoxazole and sulfamethoxazole-trimethoprim (P<0.05), but not to quinolones and amoxicillin/clavulanic acid. The conclusion was that E.coli isolated from beef cattle were seriously resistant to antimicrobials,and integron/cassettes widely existed. The presence of integrons and the association of antimicrobial resistance determinants with transferable elements might play a crucial role in the dissemination of antibiotic resistance among E.coli. Data reported here clearly emphasized the need for a stricter application of antimicrobials restriction policies in feedlot setting.

Key words: class Ⅰ integron; gene cassettes; antimicrobial resistance; Escherichia coli

中图分类号:

S852.61^＋4

王海生, 扈瑞平, 高玉敏, 杨钟, 邓秀玲, 张建宇, 武瑞兵, 呼和巴特尔. 牛源大肠杆菌Ⅰ类整合子分子特征及其与耐药性关系的研究[J]. 中国畜牧兽医, 2014, 41(9): 63-68.

WANG Hai-sheng, HU Rui-ping, GAO Yu-min, YANG Zhong, DENG Xiu-ling, ZHANG Jian-yu, WU Rui-bing, HUHE Bateer. Study on Molecular Characterization of Class Ⅰ Integron and Integron-associated Antimicrobial Resistance in Escherichia coli from Beef Cattle[J]. , 2014, 41(9): 63-68.

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