中国畜牧兽医 ›› 2014, Vol. 41 ›› Issue (9): 136-139.

• 生理生化 • 上一篇    下一篇

饮水硼对非洲雏鸵鸟肝脏组织的影响

王为, 彭克美, 肖珂, 郑昕婷, 朱黛云, 杨智, 唐娟, 孙鹏鹏, 王静   

  1. 华中农业大学动物科技-动物医学院, 湖北武汉 430070
  • 修回日期:2014-07-08 出版日期:2014-09-20 发布日期:2014-09-24
  • 通讯作者: 彭克美 E-mail:kmpeng@sohu.com
  • 作者简介:王为(1989- ),男,四川人,硕士生,研究方向:动物组织学与胚胎学。
  • 基金资助:

    国家自然科学基金(31272517)。

Effect of Drinking Boron on Liver Tissue of Africa Ostrich Chicks

WANG Wei, PENG Ke-mei, XIAO Ke, ZHENG Xin-ting, ZHU Dai-yun, YANG Zhi, TANG Juan, SUN Peng-peng, WANG Jing   

  1. College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
  • Revised:2014-07-08 Online:2014-09-20 Published:2014-09-24

摘要: 本研究旨在探究饮水硼对非洲雏鸵鸟肝脏组织的影响。本试验选择36羽10日龄非洲雏鸵鸟,随机分成6组,每组6羽,饲喂相同的基础日粮及不同添加量的饮水硼,各组饮水中硼含量分别为0、40、80、160、320、640 mg/L。试验期80 d。试验结束后,称体重,颈动脉放血致死。处死后立即取出肝脏称重,计算肝脏指数。取肝脏标本于4%多聚甲醛常温固定,制作石蜡切片,HE染色,显微观察并照相。结果证实,低剂量的饮水硼(40、80 mg/L)有利于肝脏发育,改善肝脏组织结构,使肝血窦里的枯否氏细胞增多,肝血窦增大,最佳添加量为80 mg/L。高剂量的饮水硼(160、320和640 mg/L)则会对非洲雏鸵鸟肝脏发育产生不良影响,使肝脏组织出现明显的病理学变化。

关键词: 硼; 非洲雏鸵鸟; 肝脏; 肝血窦

Abstract: The purpose of this study was to investigate the effects of drinking boron on liver tissue of Africa ostrich chicks,36 10-day-old Africa ostrich chicks were randomly divided into 6 groups with 6 replicates in each group. The same basic diet and different doses of drinking boron were fed,boron contents in drinking water in each group were 0,40,80,160,320,640 mg/L,respectively. Test period lasted for 80 d. After the test,weighed,carotid artery bleeding to death,removed the liver after death,liver indexes were calculated. Liver specimens were placed in 4% paraformaldehyde and then embedded in paraffin. The livers were sectioned,stained by HE,and then observed and photoed under microscope. The results confirmed that low doses of drinking boron (40,80 mg/L) were in favor of liver development,improved the organizational structure of the liver,increased the Kupffer cells in the liver sinusoid,enlarged hepatic sinusoid,the optimal dosage was 80 mg/L. High doses of drinking boron (160,320 and 640 mg/L) adversely affected the development of Africa ostrich chicks' livers and made liver tissue pathological changes obviously.

Key words: boron; Africa ostrich chick; liver; hepatic sinusoid

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