水牛分子生物学技术研究新进展

摘要/Abstract

摘要： 水牛是中国南方重要的经济畜种，被国际粮农组织誉为最具开发潜力和开发价值的家畜。新兴的分子生物学技术为之带来了新的机遇和挑战。近年来，水牛分子生物学技术的研究取得了重要突破，在水牛遗传育种中显示出巨大的优势和发展潜力。作者主要从分子遗传标记、动物转基因技术、基因芯片及高通量测序技术等方面介绍了分子生物学技术在水牛遗传育种中的作用，并对其应用前景进行讨论，为水牛分子生物学技术的进一步研究奠定基础，对促进水牛产业的快速发展具有重要意义。

关键词: 水牛; 分子标记; 转基因; 基因芯片; 高通量测序

Abstract: The water buffalo (Bubalusbubalus) is an important economic specie in southern provinces that was acclaimed as the most potential and valuable development on the livestock by FAO. The new molecular biology technology will bring the new challenge and opportunity for buffalo researches in recent years. Many major breakthroughs have been made in the research area on the molecular biology technology of the water buffalo, which should reveal its great superiority and development potential on the buffalo genetics and breeding. Consequently, the molecular biology technology applied to the buffalo genetics and breeding and its prospect will be discussed, mainly including the molecular marker, gene chip, transgenic technology and high-throughput sequencing technology, etc. It will lay a foundation for further research on the molecular biology technology of the water buffalo and play an important role in promoting the rapidly development of the buffalo industry.

Key words: buffalo; molecular marker; transgene; gene chip; high-throughput sequencing

邓廷贤，庞春英，王建，杨炳壮，张秀芳，梁贤威. 水牛分子生物学技术研究新进展[J]. 中国畜牧兽医.

DENG Ting-xian, PANG Chun-ying, WANG Jian, YANG Bing-zhuang, ZHANG Xiu-fang, LIANG Xian-wei. Advances on Molecular Biology Technology of the Water Buffalo （Bubalusbubalus）[J]. .

参考文献

1 王斌. 陕南水牛微卫星DNA遗传多样性研究［D］.杨陵：西北农林科技大学, 2010.
2 王锦.水牛产奶数量性状基因多态性研究［D］.南宁：广西大学, 2008.
3 王建, 庞春英,邓廷贤, 等. 影响Fat-1转基因水牛胚胎发育因素的研究［J］. 中国畜牧兽医, 2012, 39（12）: 121～124.
4 王冬蕾, 常洪, 杨军香, 等. 8 个亚洲水牛群体的遗传结构分析［J］. 遗传, 2007, 29(9): 1103～1109.
5 邓廷贤, 庞春英, 张滢寅, 等. 脂质体法转染水牛肾脏成纤维细胞条件的优化［J］. 中国畜牧兽医, 2013, 40（5）：12～15.
6 邓继贤, 莫毅, 杨秀荣, 等. 水牛Follistatin cDNA克隆及序列分析［J］. 广西农业科学, 2008, 27(3)：187～192.
7 田万强, 张道义, 林清, 等. 分子遗传标记与家畜育种进展［J］. 中国牛业科学, 2008, 34(5): 40～43.
8 史喜菊, 夏春, 汪明, 等. 水牛α-干扰素基因的克隆与表达及其抗病毒活性［J］. 农业生物技术学报, 2004, 12(3): 288～293.
9 叶鼎承. 福安水牛微卫星DNA遗传多样性分析［D］. 福州：福建农林大学, 2007.
10 刘建忠, 田为宇. 水牛α-乳清蛋白基因的克隆及序列分析［J］. 西北农业学报, 2009, 18(5): 31～34.
11 李恭贺, 雷利, 何宝祥, 等. 广西沼泽型水牛IL-1β基因的克隆及在发情周期内水平动态变化［J］. 中国兽医杂志, 2010, 46(2): 27～32.
12 李聪, 孙东晓, 姜力, 等. 奶牛重要经济性状全基因组关联分析研究进展［J］. 遗传, 2012, 34(5): 545～550.
13 齐国强, 昝林森, 张桂香, 等. 中国部分地方水牛品种mtDNA D-loop区遗传多样性与起源研究［J］. 畜牧兽医学报, 2008, 39(1): 7～11.
14 阳玉梅, 黄维义, 代华龙, 等. 广西沼泽型水牛IL-4和IL-5基因的克隆及其序列分析［J］. 中国预防兽医学报, 2007, 29(10): 768～772.
15 陈宝剑, 蒋钦杨, 毛献宝, 等. 沼泽型水牛促卵泡素基因G亚基的克隆与序列分析［J］. 广西农业科学, 2001, 40(12): 1559～1602.
16 杜挺媛, 卢克焕, 杨华, 等. 水牛垂体催乳素cDNA的克隆与序列分析［J］. 广西农业科学, 2009, 40(8): 1074～1078.
17 庞春英, 邓廷贤, 梁贤威, 等. 水牛Fat-1基因打靶载体的构建［J］.畜牧与兽医, 2012, 44(9): 50～52.
18 岳桂东, 高强, 罗龙海, 等. 高通量测序技术在动植物研究领域中的应用［J］. 中国科学C辑:生命科学, 2012, 42(2): 107～124.
19 张毅. 中国水牛分子遗传多样性及其起源分化的研究［D］. 北京: 中国农业大学, 2006.
20 徐贤坤, 熊毅, 龙剑明, 等. 水牛IFN-γ成熟蛋白基因的原核表达及其产物多克隆抗体的制备［J］. 中国兽医科学, 2008, 38(11): 1003～1008.
21 章纯熙, 吴文彩, 邹隆树,等. 中国水牛科学［M］. 南宁: 广西科学技术出版社, 2000.
22 谢体三, 农微, 张新民, 等. 水牛抑制素α亚基基因的克隆与原核表达［J］. 黑龙江畜牧兽医, 2009, 8: 14～18.
23 姜一, 刘建忠. 水牛乳蛋白基因3 端调控区的PCR克隆及序列分析［J］. 辽宁农业科学, 2009, 2: 28～30.
24 樊宝良, 张庆桥, 李宁, 等. 水牛α-乳清蛋白基因部分序列的测定与分析［J］. 中国畜牧杂志, 2005, 41(7): 21～24.
25 腾晓坤, 肖华胜. 基因芯片与高通量DNA 测序技术前景分析［J］. 中国科学C辑:生命科学, 2008, 38(10): 891～899.
26 Abdel Dayem A M H, Mahmoud K M, Nawito M F, et al. Oenotyping of kappa-casein gene in Egyptian buffalo bulls ［J］. Livestock Science, 2009, 122(2～3): 286～289.
27 Balteanu V A, Carsai T C, Vlaic A. Identification of an intronic regulatory mutation at the buffalo αS1-casein gene that triggers the skipping of exon 6［J］. Mol Biol Rep, 2013, 40(7): 4311～4316.
28 Bonfatti V, Giantin M, Gervaso M, et al. Effect of CSN1S1-CSN3 (α(S1)-κ-casein) composite genotype on milk production traits and milk coagulation properties in Mediterranean water buffalo［J］. J Dairy Sci, 2012, 95(6): 3435～3443.
29 Bruford M W, Bradley D G, Luikart G. DNA markers reveal the complexity of livestock domestication ［J］. Nature Reviews Genetics, 2003, 4: 900～910.
30 Burgess H P. Human disease: Next-generation sequencing of the next generation ［J］. Nat Rev Genet, 2011, 12(2):78.
31 Costa V, Angelini C, De Feis I, et al. Uncovering the complexity of transcriptomes with RNA-Seq［J］.J Biomed Biotechnol, 2010, 2010: 853916.
32 Damiani G, Florio S, Budelli E, et al. Single nucleotide polymorphisms (SNPs) within Bov-A2 SINE in the second intron of bovine and buffalo k-casein (CSN3) gene［J］. Anim Genet, 2000, 31(4): 277～279.
33 Daniela I, John W, Tad S, et al. The buufalo genome and the application of genomics in animal management and improvement. The 10^th World Buffalo Congress and the 7^th Asian Buffalo Congress (Invited papers). Thailand. 2013.
34 Davey J W, Hohenlohe P A, Etter P D, et al. Genome-wide genetic marker discovery and genotyping using next-generation sequencing［J］. Nat Rev Genet, 2011, 12(7): 499～510.
35 FAO. http://www.fao.org/home/en/. 2013.
36 Gordon J W, Ruddle F H. Integration and stable germ line transmission of genes injected into mouse pronuclei ［J］. Science, 1981, 214: 1244～1246.
37 Javed R, Gautam S K, Vijh R K, et al. Characterization of PRLR and PPARGC1A genes in buffalo (Bubalusbubalis)［J］. Genet Mol Biol, 2011, 34(4): 592～594.
38 Lei C Z, Zhang W, Chen H, et al. Independent maternal origin of Chinese swamp buffalo (Bubalusbubalis)［J］.Anim Genet, 2007, 38(2): 97～102.
39 Lei C Z, Zhang C M, Weining S, et al. Genetic diversity of mitochondrial cytochrome b gene in Chinese native buffalo［J］. Anim Genet, 2011, 42(4): 432～436.
40 Lucas M O, Nedenia B S, Bruna C M, et al. Alleic variant of the DRB3 gene by PCR-RFLP in Mediterranean buffaloes breed. The 10th World Buffalo Congress and the 7th Asian Buffalo Congress (Abstracts). Thailand. 2013.
41 Majeed R, Nadeem A, Babar M E, et al. POU1 transcription factor 1 DNA polymorphism in NiliRavi buffalo. In: The 10^th World Buffalo Congress and the 7^th Asian Buffalo Congress (Abstracts). Thailand. 2013.
42 Maryam J, Asif N, Masroor E B. Bovine stearoyl-coadesaturase gene polymorphism in niliravi buffalo. The 10^th World Buffalo Congress and the 7^th Asian Buffalo Congress (Abstracts).Thailand. 2013.
43 McEvoy T G, Sreenan J M. The efficiency of production, centrifugation, microinjection and transfer of one-and two-cell bovine ova in a gene transfer program ［J］. Theriogenology, 1990, 33(4): 819～828.
44 Meaburn E, Schulz R. Next generation sequencing in epigenetics: Insights and challenges ［J］. Semin Cell Dev Biol, 2012, 23(2): 192～199.
45 Michelizzi V N, Dodson M V, Pan Z, et al. Water buffalo genome science comes of age［J］. International Journal of Biological Sciences, 2010, 6(4): 333～349.
46 Minj A, Mondal S, Tiwari A K, et al. Molecular characterization of follicle stimulating hormone receptor (FSHR) gene in the Indian river buffalo (Bubalus bubalis)［J］. Gen Comp Endocrinol, 2008, 158(2): 147～153.
47 Morin R D, Zhao Y, Prabhu A L,et al. Preparation and analysis of microRNA libraries using the Illumina massively parallel sequencing technology［J］. Methods Mol Biol, 2010, 650: 173～199.
48 Mustafa H, Abdullah M, Ali A, et al. Polymorphism identification of Pit1 gene in three indigenous buffalo breeds(Nili-Ravi,Kundi&Azakhali) in Pakistan. The 10^th World Buffalo Congress and the 7th Asian Buffalo Congress (Abstracts). Thailand. 2013.
49 Nadeem A, Majeed R, Babar M E, et al. Genetic variants of POU1F1 gene in Azakheli buffalo breed of Pakistan. In: WisitipornSuksombat, RangsunParnpai, MarienaKetudat-Cairns, KanokwanSrirattana, KanchanaPunyawai, AthipLimcharoen, eds. The 10^th World Buffalo Congress and the 7^th Asian Buffalo Congress (Abstracts). Thailand. 2013.
50 P V V, Brahma B, Kaur R, et al. Characterization of β-casein gene in Indian riverine buffalo［J］. Gene, 2013, 527(2): 683～688.
51 Raut A A, Kumar A, Kala S N, et al. Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP［J］. Genet Mol Biol, 2012, 35(3): 610～613.
52 Ren D X, Miao S Y, Chen Y L, et al. Genotyping of the K-casein and β-lactoglobulin genes in Chinese Holstein, Jersey and water buffalo by PCR-RFLP［J］. J Genet, 2011, 90(1): e1～e5.
53 Shi D S, Wang J, Yang Y, et al. DGAT1, GH, GHR, PRL and PRLR polymorphism in water buffalo (Bubalus bubalis)［J］.Reprod Domest Anim, 2012, 47(2): 328～334.
54 Stafuzzza N B, Kochan K J, Riggs P K, et al. Single nucleotide variations in the buffalo κ-casein gene (CSN3). In： The 10^th World Buffalo Congress and the 7^th Asian Buffalo Congress (Abstracts). Thailand. 2013.
55 Sukumar K, Abhijit M. Characterization and expression profile of complete functional domain of granulysin/NK-lysin homologue (buffalo-lysin) gene of water buffalo (Bubalus bubalis)［J］. Veterinary Immunology lmmunopathology, 2009, 128(4): 413～417.
56 Suneel K O, Sharma D, Singh D, et al. CYP19 (cytochrome P450 aromatase) gene polymorphism in murrah buffalo heifers of different fertility performance［J］. Res Vet Sci, 2009, 86(3): 427～437.
57 Tantia M S, Vijh R K, Mishra B P, et al. DGAT1 and ABCG2 polymorphism in Indian cattle (Bosindicus) and buffalo (Bubalus bubalis) breeds［J］. BMC Vet Res, 2006, 2: 32.
58 Wu J J, Song L J, Wu F J, et al. Investigation of transferability of BovineSNP50 BeadChip from cattle to water buffalo for genome wide association study ［J］. Mol Biol Rep, 2013, 40: 743～750.
59 Yang B Z, Liang X W, Qin J, et al. Brief introduction to the development of Chinese dairy buffalo industry. The 10^th World Buffalo Congress and the 7^th Asian Buffalo Congress (Invited papers). Thailand. 2013.
60 Yuan J, Zhou J, Deng X, et al. Molecular cloning and single nucleotide polymorphism detection of buffalo DGAT1 gene［J］. Biochem Genet, 2007, 45(7～8): 611～621.
61 Zhang Y, Sun D, Yu Y, et al. Genetic diversity and differentiation of Chinese domestic buffalo based on 30 microsatellite markers ［J］. Anim Genet, 2007, 38(6): 569～575.

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