流式细胞术在霉菌毒素研究中的应用

摘要/Abstract

摘要： 世界范围内受霉菌毒素污染的农作物越来越多,这不仅造成巨大的经济损失,也给人和动物健康带来严重危害.近年来,由于流式细胞术能够对混合物中的单个细胞进行快速准确的分析,而逐步成为研究霉菌毒素细胞毒性作用的一种合理可行的方法.作者主要介绍了流式细胞术在检测霉菌毒素及其细胞毒性作用研究中的应用,以期为进一步探究霉菌毒素的毒性作用机制提供参考.

关键词: 流式细胞术; 霉菌毒素; 细胞毒性

Abstract: There are more and more mycotoxin-contaminated crops around the world, which not only results in huge economic losses, but also has adverse effects on animal and human health.Flow cytometry has become an available tool in cytotoxicity of mycotoxins studies in recent years for the rapid and accurate analysis of single cells in a mixture.The author reviewed the application of flow cytometry to determination of mycotoxins and research of its cytotoxic effect in order to provide references for further research of mycotoxin toxicity mechanism.

Key words: flow cytometry; mycotoxins; cytotoxicity

中图分类号:

董双, 何剑斌, 关心, 龙淼. 流式细胞术在霉菌毒素研究中的应用[J]. , 2014, 41(12): 258-262.

DONG Shuang, HE Jian-bin, GUAN Xin, LONG Miao. Application of Flow Cytometry to Study Mycotoxins[J]. , 2014, 41(12): 258-262.

参考文献

1 丁举静.脱氧雪腐镰刀菌烯醇诱导仓鼠肾细胞凋亡信号转导机制的研究[D].南京:南京农业大学,2009.
2 李泳宁,吴海燕,郑允权,等.应用流式微球检测黄曲霉毒素B₁方法的建立[J].福州大学学报,2012,40(1):126～131.
3 郭延锋,高均伟,朱国坡,等.细胞凋亡常用检测方法的研究进展[J].中国畜牧兽医,2010,37(2):90～92.
4 袁莉芸,朱丽,郭成志,等.玉米赤霉烯酮对猪卵巢颗粒细胞毒性的研究[J].中国兽医学报,2013,33(5):710～714.
5 戴玉奕.脱氧雪腐镰刀菌烯醇对鸡软骨细胞增殖、分化及凋亡的影响[D].南京:南京农业大学,2010.
6 Alvarez-Barrientos A,Arroyo J,Cantón R,et al.Applications of flow cytometry to clinical microbiology[J].Clinical Microbiology Reviews,2000,13(2):167～195.
7 Banjerdpongchai R,Kongtawelert P,Khantamat O,et al.Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis of human leukemic cells[J].Journal of Hematology & Oncology,2010,3(1):1～16.
8 Bensassi F,Gallerne C,Sharaf El Dein O,et al.Cell death induced by the Alternaria mycotoxin alternariol[J].Toxicology in vitro,2012,26(6):915～923.
9 Bouaziz C,Martel C,Golli E E,et al.Molecular events involved in ochratoxin A induced mitochondrial pathway of apoptosis,modulation by Bcl-2 family members[J].Environmental Toxicology,2011,26(6):579～590.
10 Bryden W L.Mycotoxin contamination of the feed supply chain:Implications for animal productivity and feed security[J].Animal Feed Science and Technology,2012,173(1):134～158.
11 Chang C,Zhu Y Q,Mei J J,et al.Involvement of mitochondrial pathway in NCTD-induced cytotoxicity in human hepG2 cells[J].J Exp Clin Cancer Res,2010,29:145.
12 Fornelli F,Minervini F,Mulè G.Cytotoxicity induced by nivalenol,deoxynivalenol,and fumonisin B₁ in the SF-9 insect cell line[J].In vitro Cellular & Developmental Biology-Animal,2004,40(5～6):166～171.
13 Henry C M,Hollville E,Martin S J.Measuring apoptosis by microscopy and flow cytometry[J].Methods,2013,61(2):90～97.
14 Hirt B V.Mathematical modelling of cell cycle and telomere dynamics[D].Nottingham:University of Nottingham,2013.
15 Kotogány E,Dudits D,Horváth G V,et al.A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine[J].Plant Methods,2010,6(1):5.
16 Krishnaswamy R,Devaraj S N,Padma V V.Lutein protects HT-29 cells against deoxynivalenol-induced oxidative stress and apoptosis:Prevention of NF-κB nuclear localization and down regulation of NF-κB and cyclo-oxygenase-2 expression[J].Free Radical Biology and Medicine,2010,49(1):50～60.
17 Krutzik P O,Irish J M,Nolan G P,et al.Analysis of protein phosphorylation and cellular signaling events by flow cytometry:Techniques and clinical applications[J].Clinical Immunology,2004,110(3):206～221.
18 Krysko D V,Vanden Berghe T,D'Herde K,et al.Apoptosis and necrosis:Detection,discrimination and phagocytosis[J].Methods,2008,44(3):205～221.
19 Li Y,Wu H,Guo L,et al.Microsphere-based flow cytometric immunoassay for the determination of citrinin in red yeast rice[J].Food Chemistry,2012,134(4):2540～2545.
20 Marin S,Ramos A J,Cano-Sancho G,et al.Mycotoxins:Occurrence,toxicology,and exposure assessment[J].Food and Chemical Toxicology,2013,60:218～237.
21 Mehrzad J,Klein G,Kamphues J,et al.In vitro effects of very low levels of aflatoxin B₁ on free radicals production and bactericidal activity of bovine blood neutrophils[J].Veterinary Immunology and Immunopathology,2011,141(1):16～25.
22 Minervini F,Lacalandra G M,Filannino A,et al.Toxic effects induced by mycotoxin fumonisin B₁ on equine spermatozoa:Assessment of viability,sperm chromatin structure stability,ROS production and motility[J].Toxicology in vitro,2010,24(8):2072～2078.
23 Perelman A,Wachtel C,Cohen M,et al.JC-1:Alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry[J].Cell Death & Disease,2012,3(11):e430.
24 Perfetto S P,Chattopadhyay P K,Roederer M.Seventeen-colour flow cytometry:Unravelling the immune system[J].Nature Reviews Immunology,2004,4(8):648～655.
25 Sawai H,Domae N.Discrimination between primary necrosis and apoptosis by necrostatin-1 in Annexin V-positive/propidium iodide-negative cells[J].Biochemical and Biophysical Research Communications,2011,411(3):569～573.
26 Shapiro H M.Practical flow cytometry[M].Boston:John Wiley & Sons,Inc.,2003.
27 Solhaug A,Vines L L,Ivanova L,et al.Mechanisms involved in alternariol-induced cell cycle arrest[J].Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis,2012,738:1～11.
28 Telford W G,Hawley T,Subach F,et al.Flow cytometry of fluorescent proteins[J].Methods,2012,57(3):318～330.
29 Vanden Berghe T,Grootjans S,Goossens V,et al.Determination of apoptotic and necrotic cell death in vitro and in vivo[J].Methods,2013,61(2):117～129.
30 Waché Y J,Hbabi-Haddioui L,Guzylack-Piriou L,et al.The mycotoxin deoxynivalenol inhibits the cell surface expression of activation markers in human macrophages[J].Toxicology,2009,262(3):239～244.
31 Wang D,Ma Q,Zhang N,et al.Genistein inhibit the proliferation induced by zearalenone in MCF-7 cells[J].Molecular & Cellular Toxicology,2010,6(1):25～31.
32 Zain M E.Impact of mycotoxins on humans and animals[J].Journal of Saudi Chemical Society,2011,15(2):129～144.

[1]	郭红伟, 蔡永超, 樊春光, 郝凯旋, 常娟, 王平, 刘超齐, 尹清强. 复合生物解毒剂在人工胃肠液中对黄曲霉毒素B₁降解率的影响[J]. 中国畜牧兽医, 2023, 50(4): 1395-1403.
[2]	唐庆凤, 李玲, 黄加祥, 方文远, 农皓如, 唐艳, 陆呈委, 韦盘秋, 冯玲. 广西地区不同饲养模式及不同季节水牛奶霉菌毒素污染状况调查[J]. 中国畜牧兽医, 2022, 49(9): 3665-3675.
[3]	潘晨浩, 张欣, 赵瑞利, 姜轩, 金天明, 于恩远, 李留安, 曾君, 于晓雪, 宋淇淇, 胡烨. 猪链球菌重组靶向表位蛋白DCpep-GSE的构建及细胞毒性检测[J]. 中国畜牧兽医, 2021, 48(8): 2990-3001.
[4]	张欣昕, 张福金, 张尧, 郭志刚, 孙林, 刘广华, 莎娜. 中国青贮玉米中霉菌毒素的污染情况分析与动物健康风险评估[J]. 中国畜牧兽医, 2021, 48(12): 4451-4459.
[5]	黄珂, 温晓鹿, 王丽, 蒋宗勇. 霉菌毒素对畜禽的危害及削减方法研究进展[J]. 中国畜牧兽医, 2019, 46(9): 2600-2607.
[6]	范彩云, 苏娣, 李晓娇, 黄帅, 张养东, 郑楠, 刘光磊, 刘祯, 程建波. 黄曲霉毒素B₁、赭曲霉毒素A和玉米赤霉烯酮联合对奶山羊肠道微生物的影响[J]. 《中国畜牧兽医》, 2017, 44(12): 3418-3425.
[7]	吴梦, 丁玉春, 刘作华, 刘文, 罗林, 葛良鹏. 猪精子质量的流式细胞检测分析[J]. 《中国畜牧兽医》, 2016, 43(9): 2502-2507.
[8]	闫宝琪, 董书伟, 王东升, 那立冬, 桑梦琪, 杨洪早, 张世栋, 严作廷. 黄藤素对山羊子宫内膜上皮细胞的体外毒性研究[J]. 《中国畜牧兽医》, 2016, 43(12): 3239-3244.
[9]	刘宁, 杨在宾, 杨维仁, 姜淑贞, 张桂国, 杨玲, 牛群升. 自然霉变玉米和玉米蛋白粉对仔猪生长性能、养分表观消化率及血清酶水平的影响[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(3): 583-588.
[10]	方思敏，柏凡，柏雪，郭春华，张正帆，彭忠利. 霉菌毒素对蛋鸡的危害及其残留与检测方法的研究进展[J]. 中国畜牧兽医, 2014, 41(6): 103-107.
[11]	乔霞，杜军，周学章. 霉菌毒素降解剂的研究进展[J]. 中国畜牧兽医, 2014, 41(1): 108-112.
[12]	郑楠, 李松励, 许晓敏, 王加启. 牛奶中霉菌毒素风险排序[J]. , 2013, 40(S1): 9-11.
[13]	杨宇航, 王文玉, 刘红娜, 刘东旭, 时坤, 李健明, 杜锐. 细胞活力及细胞免疫力检测方法的研究进展[J]. , 2013, 40(6): 142-145.
[14]	谢晓鹏, 易卫, 庄智明, 郑教雀, 邹柳, 徐茜, 彭梦华. 饲料中的霉菌毒素及其防制措施[J]. , 2013, (5): 101-106.
[15]	郑楠;王加启;韩荣伟;许晓敏;甄云鹏;屈雪寅;赵连生. 牛奶中主要霉菌毒素毒性的研究进展[J]. , 2012, 39(3): 10-13.