高分辨率熔解曲线分析应用的研究进展

摘要/Abstract

摘要： 高分辨率熔解曲线分析(high-resolution melting curve analysis,HRMA)是结合饱和荧光染料、未标记探针和实时荧光定量PCR的一种新的检测基因突变与基因分型的分子诊断技术,具有高通量、低成本、简单快捷、结果准确、灵敏度和特异性高及真正闭管操作等优点。作者就高分辨率熔解曲线分析应用的研究进展进行综述。

关键词: 突变检测; 熔解曲线分析; 核酸

Abstract: High resolution melting analysis (HRMA) was a new molecular diagnosis method for mutation scanning and genotyping, which combined special saturated fluorescent dyes, unlabled probe and Real-time PCR. High resolution melting analysis was superior to other nucleic acid research methods, including an inexpensive closed tube format that was a rapid, accurate, sensitive, special and high-throughput assay. In this paper, advancement on the application of high resolution melting in molecular diagnosis was summarized.

Key words: mutation detection; melting curve analysis; nucleic acid

中图分类号:

S816.79

刘自增, 吴周良, 阎萍, 王一文. 高分辨率熔解曲线分析应用的研究进展[J]. 中国畜牧兽医, 2013, 40(8): 105-111.

LIU Zi-zeng, WU Zhou-liang, YAN Ping, WANG Yi-wen. Research Progress on the Application of High-resolution Melting Curve Analysis[J]. , 2013, 40(8): 105-111.

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