PRRSV野毒感染猪群免疫接种弱毒活疫苗的效果分析

doi:10.16431/j.cnki.1671-7236.2016.09.033

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (9): 2447-2454.doi: 10.16431/j.cnki.1671-7236.2016.09.033

PRRSV野毒感染猪群免疫接种弱毒活疫苗的效果分析

许心婷¹, 施开创², 李常挺³, 龙飞翔¹, 莫胜兰², 陈汉忠¹

1. 广西大学动物科学技术学院, 南宁 530005;
2. 广西动物疫病预防控制中心, 南宁 530001;
3. 隆安县动物疫病预防控制中心, 隆安 532700

收稿日期:2016-03-01 出版日期:2016-09-20 发布日期:2016-09-20
通讯作者: 施开创 E-mail:shikaichuang@126.com
作者简介:许心婷(1990-),女,广西玉林人,硕士生,研究方向:动物疫病诊断与防控,E-mail:18577818613@163.com
基金资助:
广西科学研究与技术开发计划项目（桂科转14125004-22）

Analysis of the Immune Efficacy of Attenuated Live Vaccine in the Pig Herds Previously Infected with Wild-type Porcine Reproductive and Respiratory Syndrome Virus

XU Xin-ting¹, SHI Kai-chuang², LI Chang-ting³, LONG Fei-xiang¹, MO Sheng-lan², CHEN Han-zhong¹

1. College of Animal Science and Technology, Guangxi University, Nanning 530005, China;
2. Guangxi Center for Animal Disease Control and Prevention, Nanning 530001, China;
3. Long'an Center for Animal Disease Control and Prevention, Long'an 532700, China

Received:2016-03-01 Online:2016-09-20 Published:2016-09-20

摘要/Abstract

摘要：

为评估猪繁殖与呼吸综合征（porcine reproductive and respiratory syndrome，PRRS）弱毒活疫苗的免疫效果，本研究在两个小型猪场各选取两窝仔猪，在免疫接种猪繁殖与呼吸综合征病毒（porcine reproductive and respiratory syndrome virus，PRRSV）弱毒活疫苗后不同时间采集血清，应用RT-PCR检测PRRSV核酸，应用N-ELISA和G-ELISA两种试剂盒检测PRRSV抗体。结果发现，免疫前0 d可以从仔猪血清中检测到PRRSV野毒株核酸，可持续到免疫后30 d；相对应的母猪在免疫前0 d可从血清中检测到PRRSV野毒株核酸。试验仔猪在免疫前0 d未能检测到PRRSV抗体，但在免疫后30～150 d均可检测到PRRSV抗体，其中N-ELISA试剂盒的阳性检出率在免疫后30、60 d高于G-ELISA试剂盒的阳性检出率，而在免疫后120、150 d则低于G-ELISA试剂盒的阳性检出率。使用两种ELISA试剂盒共同检测216份血清样品，检测结果的总体符合率为95.83%；配对χ²检验，P>0.05，两者差异不显著；Kappa值为0.87，属于极强一致性；两者相关系数r为0.605，具有显著线性相关。表明免疫接种弱毒活疫苗可以有效清除野毒感染猪群中的野毒株，N-ELISA和G-ELISA两种ELISA试剂盒均可用于评估弱毒活疫苗的免疫效果。

关键词: 猪繁殖与呼吸综合征; 弱毒活疫苗; 间接ELISA; RT-PCR; 抗体; 病毒核酸

Abstract:

To evaluate the immune efficacy of porcine reproductive and respiratory syndrome (PRRS) attenuated live vaccine,this study selected 4 litters from two small pig farms (two litters each farm) and vaccinated with porcine reproductive and respiratory syndrome virus (PRRSV) attenuated live vaccine.Sera were collected on different days post vaccination (dpv) to detect PRRSV nucleic acid by RT-PCR,and PRRSV antibody using two different ELISA Kits,i.e.N-ELISA and G-ELISA.The results showed that PRRSV nucleic acid were positive on 0 dpv until 30 dpv in piglets and also positive on 0 dpv in the corresponding sows.All piglets were negative for PRRSV antibody on 0 dpv,but were positive on 30 dpv until 150 dpv.The positive rates detected by N-ELISA Kit were higher than those of G-ELISA Kit on 30 and 60 dpv,but lower than those of G-ELISA Kit on 120 and 150 dpv.A total of 216 sera were detected respectively by two ELISA Kits and the coincidence rate of the results was 95.83%.The P value of χ² test was more than 0.05,showing there was no significant difference between the results of two Kits.The Kappa value was 0.87,showing there was strong consistence between them.The correlation coefficient was 0.605,showing there was significantly linear correlation between them.The results indicated that the wild-type PRRSV in the previously infected pig herds could be eliminated by vaccination with attenuated live vaccine and both N-ELISA and G-ELISA Kits could be used to estimate the immune efficacy of the attenuated live vaccine effectively.

Key words: porcine reproductive and respiratory syndrome (PRRS); attenuated live vaccine; indirect ELISA; RT-PCR; antibody; viral nucleic acid

中图分类号:

S855.3

许心婷, 施开创, 李常挺, 龙飞翔, 莫胜兰, 陈汉忠. PRRSV野毒感染猪群免疫接种弱毒活疫苗的效果分析[J]. 《中国畜牧兽医》, 2016, 43(9): 2447-2454.

XU Xin-ting, SHI Kai-chuang, LI Chang-ting, LONG Fei-xiang, MO Sheng-lan, CHEN Han-zhong. Analysis of the Immune Efficacy of Attenuated Live Vaccine in the Pig Herds Previously Infected with Wild-type Porcine Reproductive and Respiratory Syndrome Virus[J]. , 2016, 43(9): 2447-2454.

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PRRSV野毒感染猪群免疫接种弱毒活疫苗的效果分析

Analysis of the Immune Efficacy of Attenuated Live Vaccine in the Pig Herds Previously Infected with Wild-type Porcine Reproductive and Respiratory Syndrome Virus

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