RPA检测技术在禽源病原检测中的研究进展

doi:10.16431/j.cnki.1671-7236.2024.02.005

摘要/Abstract

摘要： 重组酶聚合酶扩增(recombinant polymerase amplification,RPA)是一种近年来研究者关注较多的新型恒温核酸扩增技术,该方法操作便捷、扩增速度快、特异性强及灵敏度高,无需借助精密仪器,且在恒温环境下(25~45 ℃)即可完成核酸检测,极有可能取代传统PCR检测技术。RPA检测技术迄今发展十余年,已广泛应用于细菌、病毒、真菌、寄生虫及耐药基因检测等领域,被认为是当前最有潜力的快速分子诊断工具,具有较为广泛的应用前景。笔者就RPA检测、技术优势、常见检测方式及其在禽源病原检测方面的研究进展进行概述,旨在为临床禽源病原新型检测技术的研究提供一定理论参考。

关键词: 重组酶聚合酶扩增(RPA); 禽源病原; 恒温核酸扩增技术

Abstract: Recombinant polymerase amplification (RPA) is a new type of constant temperature nucleic acid amplification technology that has attracted more attention from researchers in recent years.Due to its convenient operation, fast amplification speed, high specificity, and high sensitivity, without the need for precision instruments, and being able to complete nucleic acid detection in a constant temperature environment (25-45 ℃), it is highly likely to replace traditional PCR detection technology.Since the development of RPA for over a decade, it has been widely applied in the fields of bacteria, viruses, fungi, parasites, and drug resistance.This method has been recognized by researchers as the most promising rapid molecular diagnostic tool currently, it has a wide range of application prospects.The author summarized the RPA detection, technological advantages, common detection methods, and research progress in the detection of avian pathogens, aiming to provide theoretical reference for the research of new detection technologies for clinical avian pathogens.

Key words: recombinant polymerase amplification (RPA); avian pathogens; constant temperature nucleic acid amplification technology

中图分类号:

S858.3

包涛涛, 杨先富, 廖飞, 吴通奎, 莫兴虎, 汪忠荣. RPA检测技术在禽源病原检测中的研究进展[J]. 中国畜牧兽医, 2024, 51(2): 482-490.

BAO Taotao, YANG Xianfu, LIAO Fei, WU Tongkui, MO Xinghu, WANG Zhongrong. Research Progress of RPA Detection Technology in the Detection of Avian Pathogens[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 482-490.

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