口蹄疫病毒分子生物学检测方法研究进展

摘要/Abstract

摘要： 口蹄疫是一种高度传染的病毒性疫病,及早诊断意义重大。分子生物学检测方法在口蹄疫病诊断和病毒分型过程中具有许多优势,已经广泛应用。作者对实时荧光PCR法、多重PCR法、核酸杂交法、基因芯片法、环介导等温扩增法及核酸序列依赖扩增法等进行分析和比较,对各种方法的检测效率、灵敏度、优缺点及国内外的研究情况进行综述,为口蹄疫病毒分子生物学检测方法的应用与深入开发提供参考。

关键词: 口蹄疫病毒; 基因检测; 基因分型

Abstract: It is of great significance to diagnose the foot-and-mouth disease early, which is a highly contagious disease. Molecular biological detection methods with many advantages, such as the Real-time fluorescent PCR, the multiple PCR, the nucleic acid hybridization, the gene chip, the loop-mediated isothermal amplification and the nucleic acid sequence-based amplification,have been widely used in the diagnosis and classification. The analysis and comparison about the detection efficiency, sensitivity, advantages and disadvantages as well as the research status at home and abroad were reviewed in this study and provided the reference for the molecular biology detection of foot and mouth disease virus applied to in-depth development.

Key words: foot-and-mouth disease virus; gene detection; genotyping

中图分类号:

S852.65

孙芳芳, 董英, 薛强, 邹明强. 口蹄疫病毒分子生物学检测方法研究进展[J]. , 2013, 40(6): 190-194.

SUN Fang-fang, DONG Ying, XUE Qiang, ZOU Ming-qiang. Research Progress on the Molecular Biology Techniques in Detection of Foot and Mouth Disease Virus[J]. , 2013, 40(6): 190-194.

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