羊毛尾线虫线粒体nad4基因的序列变异分析

›› 2013, Vol. 40 ›› Issue (4): 81-84.

羊毛尾线虫线粒体nad4基因的序列变异分析

王燕^1,2, 杨言川², 张悦文^2,3, 李佳缘^1,2, 朱兴全², 刘国华⁴

1. 华南农业大学兽医学院,广东广州 510642;
2. 中国农业科学院兰州兽医研究所, 家畜疫病病原生物学国家重点实验室,甘肃兰州 730046;
3. 西交利物浦大学,江苏苏州 215123;
4. 湖南农业大学动物医学院,湖南长沙 410128

收稿日期:2012-08-15 出版日期:2013-04-20 发布日期:2013-04-19
通讯作者: 刘国华。
作者简介:王燕(1988-),女,四川人,研究生,研究方向:寄生虫分子生物学。
基金资助:
家畜疫病病原生物学国家重点实验室开放基金(SKLVEB2010KFKT010、SKLVEB2011KFKT004);国家肉牛牦牛产业技术体系项目(CARS-38)。

Analysis of Sequence Variation in Mitochondrial nad4 Gene of Trichuris ovis Isolates

WANG Yan^1,2, YANG Yan-chuan², ZHANG Yue-wen^2,3, LI Jia-yuan^1,2, ZHU Xing-quan², LIU Guo-hua⁴

1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642,China;
2. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046,China;
3. Xi’an Jiaotong-Liverpool University, Suzhou 215123,China;
4. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China

Received:2012-08-15 Online:2013-04-20 Published:2013-04-19

摘要/Abstract

摘要： 本研究旨在阐明中国羊毛尾线虫(Trichuris ovis)广东分离株线粒体NADH脱氢酶亚单位4(nad4)基因部分序列(pnad4)的遗传变异情况, 并用pnad4序列构建其与其他鞭虫的进化关系。应用PCR扩增羊鞭虫虫株的pnad4,将所获得的序列应用ClustalX 1.81程序进行比对,然后用Mega 5.0程序NJ法绘制种系发育树。本试验扩增所获得的pnad4序列长度一致,均为827 bp,种内变异在0~1.5%之间。种系发育分析结果表明,12个羊鞭虫分离株位于同一分支。由于羊鞭虫nad4序列种内相对保守,种间差异较大(37.8%~45.6%), 故可作为种间鉴定检测研究的遗传标记, 本研究结果为进一步研究羊鞭虫的群体遗传结构奠定了基础。

关键词: 羊鞭虫; 线粒体DNA; nad4基因; 种系发育关系

Abstract: The objectives of the present study were to analyze sequence variation in the NADH dehydrogenase subunit 4 (nad4) gene among Trichuris ovis isolates from Guangdong province in China, and to study its phylogenetic relationships with other whipworms using the nad4 gene sequences. The partial nad4 (pnad4) were amplified from individual T.ovis samples, and pnad4 sequences were aligned using the ClustalX 1.81. Neighbor-joining (NJ) trees were constructed using the software Mega version 5.0. The lengths of all pnad4 sequences were 827 bp. Phylogenetic analyses showed that all T.ovis isolates were clustered in the same clade.Sequence variations in pnad4 sequences within T.ovis were 0 to 1.5%, which was significantly lower than inter-species differences (37.8% to 45.6%). It was concluded that pnad4 sequence could be used as a genetic marker for population genetic studies of T.ovis, as well as for the differentiation and identification of different whipworms. The results of this study provided foundation for further studies on molecular genetics and diagnostics of T.ovis.

Key words: Trichuris ovis; mitochondrial DNA; NADH dehydrogenase subunit 4 gene; phylogenetic relationship

中图分类号:

王燕, 杨言川, 张悦文, 李佳缘, 朱兴全, 刘国华. 羊毛尾线虫线粒体nad4基因的序列变异分析[J]. , 2013, 40(4): 81-84.

WANG Yan, YANG Yan-chuan, ZHANG Yue-wen, LI Jia-yuan, ZHU Xing-quan, LIU Guo-hua. Analysis of Sequence Variation in Mitochondrial nad4 Gene of Trichuris ovis Isolates[J]. , 2013, 40(4): 81-84.

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