广西5个猪种和国外3个猪种mtDNA D-loop序列遗传多样性及进化分析

doi:10.16431/j.cnki.1671-7236.2015.07.027

›› 2015, Vol. 42 ›› Issue (7): 1807-1815.doi: 10.16431/j.cnki.1671-7236.2015.07.027

广西5个猪种和国外3个猪种mtDNA D-loop序列遗传多样性及进化分析

张冰^1,2, 廖海洪^1,2, 农素群², 马青艳², 孙俊丽^1,2

1. 广西家畜遗传改良重点实验室, 南宁 530001;
2. 广西壮族自治区畜牧研究所, 南宁 530001

收稿日期:2014-11-06 出版日期:2015-07-20 发布日期:2015-07-23
通讯作者: 孙俊丽 E-mail:sjn313@126.com
作者简介:张冰(1979-),男,河南鲁山人,硕士,助理研究员,研究方向:动物分子遗传育种,E-mail:zbcherry@126.com
基金资助:
广西自然科学基金项目(2012GXNSFAA053071、2013GXNSFB019115)

The Genetic Diversity and Phylogenetic Relationships of Five Guangxi Pig Breeds and Three Exotic Pig Breeds by Analysis of mtDNA D-loop Sequences

ZHANG Bing^1,2, LIAO Hai-hong^1,2, NONG Su-qun², MA Qing-yan², SUN Jun-li^1,2

1. Guangxi Key Laboratory of Livestock Genetic Improvement, Nanning 530001, China;
2. Guangxi Institute of Animal Science, Nanning 530001, China

Received:2014-11-06 Online:2015-07-20 Published:2015-07-23

摘要/Abstract

摘要： 为了比较广西猪种和国外3个猪种的遗传资源多样性和进化关系,利用文献报道的1对引物测定了广西5个地方猪种(巴马香猪、东山猪、桂中花猪、陆川猪、环江香猪)共85个样本的mtDNA D-loop序列,结合已报道的国外3个猪种(杜洛克猪、大约克猪和长白猪)的45条mtDNA D-loop序列,进行单倍型、遗传多样性和系统发育树分析。结果表明,8个群体样本的130条序列共定义38种单倍型;广西各猪种之间遗传距离较小,远小于其与国外猪种杜洛克猪、长白猪间的遗传距离;构建的NJ分子系统进化树将所分析样本分为2个独立的支系:一支包含了所有广西地方猪种和部分大约克猪的单倍型,另一支则全部是3个国外猪种的单倍型,广西猪种和3个国外猪种有着两个明显不同的母系起源。陆川猪在系统树上分布较为紧密,其他4个猪种都很分散,表明陆川猪母源血统单一,其他4个猪种母源血统遗传背景比较复杂,彼此之间有基因交流。广西猪种母系资源没有受到国外引进猪种资源的影响,在其开发利用中依然适合用作母本。

关键词: 广西猪种; 国外猪种; 线粒体DNA控制区; 遗传多样性; 系统进化

Abstract: In order to compare the genetic diversity and phylogenetic relationships between Guangxi pig breeds and three exotic pig breeds,one pair of primers was used to amplify 85 mtDNA D-loop sequences of five Guangxi pig breeds (Bama Xiang pig,Dongshan pig,Guizhong spotted pig,Luchuan pig,Huanjiang Xiang pig),and we downloaded 45 mtDNA D-loop sequences of three exotic pig breeds (Duroc,Yorkshire,Landrace) from GenBank.Then the haplotype,genetic diversity and phylogenetics analysis were carried out.The results showed that 38 haplotypes were identified in 130 sequences,and the mean distances among Guangxi pig breeds were smaller than that among Duroc,Landrace and Guangxi pig breeds.The NJ phylogenetic tree classified all samples into two clusters.One contained all of Guangxi pig breeds and partial Yorkshire,the other was composed wholly of exotic pig breeds,which mean there were two distinct maternal origins.In the cluster of Guangxi pig breeds,Luchuan pig were clustered,and the others were rather dispersive.It indicated that Luchuan pig had simple maternal bloods,and the other breeds had complex maternal bloods,and they had gene flow with each other.The maternal pig resources in Guangxi were not affected by the introduction of exotic pig breeds,and they were still suitable for use as a female parent in their development and utilization.

Key words: Guangxi pig breeds; exotic pig breeds; mtDNA D-loop; genetic diversity; phylogenetic relationship

中图分类号:

S813.3

张冰, 廖海洪, 农素群, 马青艳, 孙俊丽. 广西5个猪种和国外3个猪种mtDNA D-loop序列遗传多样性及进化分析[J]. , 2015, 42(7): 1807-1815.

ZHANG Bing, LIAO Hai-hong, NONG Su-qun, MA Qing-yan, SUN Jun-li. The Genetic Diversity and Phylogenetic Relationships of Five Guangxi Pig Breeds and Three Exotic Pig Breeds by Analysis of mtDNA D-loop Sequences[J]. , 2015, 42(7): 1807-1815.

参考文献

[1] 孙俊丽,张冰,马青艳,等.陆川猪mtDNA D-loop序列遗传多样性分析[J].中国畜牧兽医,2010,37(6):122-125.
[2] Grossi S F,Lui J F,Garcia J E,et al.Genetic diversity in wild (Sus scrofa scrofa) and domestic (Sus scrofa domestica) pigs and their hybrids based on polymorphism of a fragment of the D-loop region in the mitochondrial DNA[J].Genet Mol Res,2006,5(4):564-568.
[3] Royo L J,Alvarez I,Beja-Pereira A,et al.The origins of iberian horses assessed via mitochondrial DNA[J].J Hered,2005,96(6):663-669.
[4] Molnár J,Tóth G,Stéger V,et al.Mitochondrial D-loop analysis reveals low diversity in Mangalica pigs and their relationship to historical specimens[J].J Anim Breed Gene,2013,130(4):312-320.
[5] Cannon M V,Brandebourg T D,Kohn M C,et al.Mitochondrial DNA sequence and phylogenetic evaluation of geographically disparate Sus scrofa breeds[J].Anim Biotechno,2015,26(1):17-28.
[6] Sambrook J,Russell D W,Huang P T.Molecular cloning:A laboratory manual[M].3rd edition.Beijing:Science Press,2002.
[7] 刘中禄,魏泓,曾养志,等.中国三种实验用小型猪mtDNA D-loop多态性分析[J].动物学报,2001,47(4):425-430.
[8] Rozas J,Rozas R.DNAsp,DNA polymorphism analysis by the coalescent and other methods[J].Bioinformatics,2003,19(18):2496-2497.
[9] Yang J,Wang J,Kijas J,et al.Genetic diversity present within the near-completemt DNA genome of 17 breeds of indigenous Chinese pigs[J].J Hered,2003,94(5):381-385.
[10] 王继英,郭建凤,孙守礼,等.山东猪种mtDNA D-loop 区部分序列遗传多样性及系统进化研究[J].畜牧兽医学报,2009,40(6):792-799.
[11] Giuffra E,Kijas J M H,Amarger V,et al.The origin of the domestic pig:Independent domestication and subsequent introgression[J].Genetics,2000,154(4):1785-1791.
[12] Kijas J M H,Andersson L.A phylogenetic study of the origin of the domestic pig estimated from the near-complete mtDNA genome[J].J Mol Evol,2001,52(3):302-308.
[13] Kim K I,Lee J H,Li K,et al.Phylogenetic relationships of Asian and European pig breeds determined by mitochondrial D-loop sequence polymorphism[J].Animal Genetics,2002,33(1):19-25.
[14] Larson G,Dobney K,Albarella U,et al.Worldwide phylogeography of wild boar reveals multiple centers of pig domestication[J]. Science,2005,307(5715):1618-1621.
[15] 李有恒,韩德芬.广西桂林甑皮岩遗址动物群[J].古脊椎动物学报,1978,16(4):244-254.
[16] 尹帅,盛桂莲,侯新东,等.广西地区野猪化石古DNA及猪的驯化初探[J].古脊椎动物学报,2013,51(4):321-330.
[17] Yu G,Xiang H,Wang J,et al.The phylogenetic status of typical Chinese native pigs:Analyzed by Asian and European pig mitochondrial genome sequences[J].J Anim Sci Biotechnol,2013,4(1):9.
[18] 刘靖闻,张勇,赵兴绪.甘南州蕨麻猪mtDNA D-环序列的起源及遗传多样性分析[J].中国兽医学报,2013,33(6):933-938.
[19] Okumura N,Ishiguro N,Nakano M,et al.Geographic population structure and sequence divergence in the mitochondrial DNA control region of the japanese wild boar (Sus scrofa leucomystax),with reference to those of domestic pigs[J].Biochem Genet,1996,34(5-6):179-189.

广西5个猪种和国外3个猪种mtDNA D-loop序列遗传多样性及进化分析

The Genetic Diversity and Phylogenetic Relationships of Five Guangxi Pig Breeds and Three Exotic Pig Breeds by Analysis of mtDNA D-loop Sequences

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