黄酮和维生素E对己烯雌酚所致雄性小鼠肝脏氧化损伤的保护作用

摘要/Abstract

摘要： 本试验旨在探讨黄酮(flavonoids)和维生素E(VE)对己烯雌酚(diethylstilboestrol, DES)所致昆明雄性小鼠肝脏氧化损伤的保护作用。将80只小鼠随机分为正常对照组、DES模型组(0.035、0.35、3.5 mg/(kg·d))、黄酮干预组(26、58、112 mg/(kg·d))和VE干预组(56.2、112.4、224.8 mg/(kg·d));干预组先灌服3.5 mg/(kg·d)DES处理,再灌服不同剂量黄酮和VE;正常对照组灌服等体积生理盐水。灌胃给药,连续7 d,试验结束后禁食24 h,脱颈处死,剖检肝脏和睾丸。计算肝脏和睾丸脏器系数,测定肝脏组织中的丙二醛(MDA)含量、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果表明,不同剂量的DES组小鼠肝脏系数和睾丸系数均下降;112.0 mg/(kg·d)黄酮剂量组与高剂量DES组相比,SOD活性上升,MDA极显著下降(P<0.01);与高剂量DES组相比,112.4、224.8 mg/(kg·d) VE剂量组T-AOC极显著上升(P<0.01),MDA极显著下降(P<0.01),224.8 mg/(kg·d) VE剂量组GSH-PX极显著上升(P<0.01)。不同浓度的DES均导致肝脏不同程度氧化损伤,黄酮和VE均能提高机体的抗氧化能力,降低DES对机体造成的氧化损伤。

关键词: 己烯雌酚; 黄酮; 维生素E; 氧化损伤

Abstract: The purpose of this study was to investigate the oxidative damage caused by different doses of diethylstilbestrol (DES) and the protective effects for DES induced toxicity with flavonoids and vitamin E in Kunming mice. 80 Kunming male mice were randomly divided into 10 groups, the groups were normal saline control group, DES exposed groups (0.035, 0.35, 3.5 mg/(kg穌)), flavonoids interference groups(26.0, 58.0, 112.0 mg/(kg穌)) and vitamin E interference groups(56.2, 112.4, 224.8 mg/(kg穌)). The effects on liver and testis were studied in adult male mice treated for one week. Mice were killed to collect liver and testis, and then contents of malonadehyde (MDA), superoxide dismutase (SOD), glutathione perxidase (GSH-Px) and total antioxidant capacity (T-AOC) were determined. It was found that high dose DES (3.5 mg/(kg穌)) could induce mice liver oxidative injury. The protective effects of flavonoids for DES induced toxicity on liver dose dependent increased. The contents of MDA significantly decreased (P<0.01), but the contents of SOD significantly increased in the high dose group (112 mg/(kg穌)). The contents of MDA significantly decreased (P<0.01), but the contents of GSH-Px, SOD and T-AOC significantly increased in the medium and high dose groups (112.4, 224.8 mg/(kg穌)). DES appeared to cause oxidative injury in Kunming mice. The results provided preliminary experimental support for flavonoids and vitamin E as medicine for DES induces damage.

Key words: diethylstilbestrol; flavonoids; vitamin E; oxidative injury

中图分类号:

Q579.1⁺3

杜娟, 潘红艳, 林利美, 黄大伟, 宫智勇. 黄酮和维生素E对己烯雌酚所致雄性小鼠肝脏氧化损伤的保护作用[J]. , 2013, 40(4): 149-153.

DU Juan, PAN Hong-yan, LIN Li-mei, HUANG Da-wei, GONG Zhi-yong. Protection Effects of Flavonoids and Vitamin E on Diethylstilbestrol Induced Oxidative Injury[J]. , 2013, 40(4): 149-153.

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