蛋白酪氨酸磷酸酶Shp2基因mRNA在小鼠睾丸发育过程中的表达

›› 2013, Vol. 40 ›› Issue (11): 143-147.

蛋白酪氨酸磷酸酶Shp2基因mRNA在小鼠睾丸发育过程中的表达

蔡晓黎¹, 耿果霞², 刘亚伟¹, 李青旺¹

1. 西北农林科技大学动物科技学院, 动物繁殖与细胞工程实验室, 陕西杨凌 712100;
2. 西北农林科技大学动物医学院, 陕西杨凌 712100

收稿日期:2013-03-22 出版日期:2013-11-20 发布日期:2013-12-19
通讯作者: 李青旺 E-mail:liqingwangysu@aliyun.com
作者简介:蔡晓黎(1986-),女,陕西人,硕士生,研究方向:动物生殖生理调控。耿果霞(1961-),女,陕西人,硕士,高级实验师,研究方向:临床兽医。
基金资助:
农业部转基因动物研究项目(2011ZX08008-003)。

Expression of Protein Tyrosine Phosphatase Shp2 Gene mRNA in Development of Mouse Testicle

CAI Xiao-li¹, GENG Guo-xia², LIU Ya-wei¹, LI Qing-wang¹

1. Laboratory of Animal Reproduction and Cell Engineering, College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China;
2. College of Veterinary Medicine, Northwest A & F University, Yangling 712100, China

Received:2013-03-22 Online:2013-11-20 Published:2013-12-19

摘要/Abstract

摘要： 为探讨蛋白酪氨酸磷酸酶Shp2 mRNA在小鼠出生后睾丸发育过程中的表达规律,本试验以16组出生后不同发育阶段的昆明种健康小鼠的睾丸组织为材料,经Real-time PCR检测小鼠出生后睾丸中Shp2基因mRNA的表达变化。结果显示,Shp2基因mRNA在小鼠出生后睾丸发育过程中连续表达,且第20~31天出现明显波动,20 d时Shp2表达量极显著增加(P<0.01),然后下降(P<0.01),在31 d再次极显著增加(P<0.01),31 d后,Shp2的表达量又逐渐恢复平稳(P>0.05)。上述结果表明,Shp2基因的连续性表达及在特定发育阶段表达量的明显波动,预示着Shp2参与小鼠出生后睾丸发育的全过程,但具体作用如何还有待进一步研究。

关键词: 睾丸发育; 小鼠; Shp2基因; 表达规律

Abstract: In this study, we analyzed the expressional pattern of Shp2 gene on the transcriptional level in mice testicles in different developmental stages. General RT-PCR and Real-time PCR were uesd to investigate the Shp2 expression in 16 groups of postnatal mice according to the differently developmental stages. The result showed that the temporal expression of Shp2 gene in mice testicles presented successively regardless of the daily growth according to the general RT-PCR assay. The result of Real-time PCR showed that, during the period from twentieth day to thirty-first day, the expression quantity of Shp2 gene fluctuated from the extremly significantly increase (P<0.01) to extremly significantly decrease (P<0.01) to extremly significantly increase again (P<0.01), and finally, the amount was back to normal gradually after the thirty-first day (P>0.05).The results indicated that Shp2 involved in the development of mouse testis, but the functions in details needed to be explored and investigated in depth.

Key words: development of testicle; mouse; Shp2 gene; pattern of expression

中图分类号:

Q786

蔡晓黎, 耿果霞, 刘亚伟, 李青旺. 蛋白酪氨酸磷酸酶Shp2基因mRNA在小鼠睾丸发育过程中的表达[J]. , 2013, 40(11): 143-147.

CAI Xiao-li, GENG Guo-xia, LIU Ya-wei, LI Qing-wang. Expression of Protein Tyrosine Phosphatase Shp2 Gene mRNA in Development of Mouse Testicle[J]. , 2013, 40(11): 143-147.

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