贵州地方猪品种对氧磷脂酶1基因的多态性研究

›› 2013, Vol. ›› Issue (5): 144-149.

贵州地方猪品种对氧磷脂酶1基因的多态性研究

郑芳霞¹, 王嘉福^1,2, 冉雪琴¹

1. 贵州大学动物科学学院, 贵州贵阳 550025;
2. 贵州大学农业工程省重点实验室, 贵州贵阳 550025

修回日期:2013-02-04 出版日期:2013-05-20 发布日期:2013-05-27
通讯作者: 冉雪琴,教授。E-mail:as.xqran@gzu.edu.cn;Fax:0851-8298003 E-mail:as.xqran@gzu.edu.cn
作者简介:郑芳霞(1986-),女,新疆人,硕士生,研究方向:动物生物化学与分子生物学。
基金资助:
国家科技支撑计划(2008BADB5B02);贵州省科技创新人才团队建设专项(黔科合人才团队2009-4006);贵州省省长资金(黔省专合字2009-100);贵州省自然科学基金(黔科合J字2008-2129);贵州大学研究生创新基金(校研农2012038)。

Study on Polymorphism of Paraoxonase 1 Gene in Guizhou Indigenous Pig Breeds

ZHENG Fang-xia¹, WANG Jia-fu^1,2, RAN Xue-qin¹

1. School of Animal Science, Guizhou University, Guiyang 550025, China;
2. Key Laboratory for Agricultural Bioengineering of Guizhou Province, Guiyang 550025, China

Revised:2013-02-04 Online:2013-05-20 Published:2013-05-27

摘要/Abstract

摘要： 本试验以欧洲猪种为参照,研究贵州地方猪对氧磷脂酶1(paraoxonase,PON1)基因的多态性,探讨基因多态性与地方猪的脂肪沉积之间是否存在相关性。研究采用锚定聚合酶链反应技术对香猪、糯谷猪、萝卜猪和可乐猪共138个贵州地方猪品种PON1基因的单核苷酸多态性(single nucleotide polymorphisms,SNP)进行研究。结果表明,猪群PON1基因的163/165位点出现A/T、G/A变异,515位点检测到C/T突变,均以3种基因型存在。经卡方(χ²)检验,贵州地方猪群PON1基因515位点的基因型频率和基因频率与欧洲猪品种间的差异不显著(P>0.05);163/165位点中,贵州地方猪种A等位基因的频率为83.7%,明显高于欧洲猪品种(25.2%)。贵州地方猪品种PON1基因的163/165位点A等位基因占优势,编码的第55位氨基酸为甲硫氨酸(Met55),与脂代谢异常人群的基因型相同;欧洲猪种以B等位基因为主(P<0.01),编码的第55位是亮氨酸(Leu55),与正常人群的基因型一致。以人PON1蛋白的晶体结构为模板,推导出香猪PON1蛋白的三维结构。地方猪PON1基因变异导致的Met55突变可能影响PON1水解酶活性中心的疏水环境,可能使蛋白的水解酶活性下降,使得地方猪品种的脂肪沉积量和肉质不同于欧洲猪种。由此推测,贵州地方猪品种PON1基因163/165位点存在多态性,可能与地方猪种的脂肪沉积和肉质有一定的相关性。

关键词: 贵州地方猪种; 对氧磷脂酶1基因; 多态性; 三维结构; 肉质

Abstract: Taking European pig breeds as control, the single nucleotide polymorphisms of paraoxonase (PON1) gene were investigated in Guizhou indigenous pig breeds, including Xiang, Nuogu, Luobo and Kele pig breeds, using allele-specific polymerase chain reaction (AS-PCR) method. The results showed that two nucleotide mutations, A/T and G/A were found out from site 163/165, and one with C/T variation from site 515 of PON1 gene of porcine population. Three genotypes, wild, mutant, and heterozygous, of site 163/165 and 515 were detected from Guizhou indigenous pig breeds and European ones. Frequencies of genotypes and alleles at site 515 in Guizhou pig breeds were not different from that of European pig breeds (P>0.05). But it was significant at site 163/165 of PON1 gene. Allele A was dominant in Guizhou pig breeds with frequency of 83.7%, and were higher than European pig breeds (25.2%). The mutated nucleotides at site 163/165 encoded methionine (Met 55), which was consistent with that of human patients with metabolic syndrome. The wild genotype of PON1 at site 163/165 (allele B), dominated in Europe pig breeds, contained residue leucine (Leu 55) which was corresponding to population without metabolism disorder. Taking the crystal structure of human PON1 protein as template, three-dimensional structure of PON1 from Xiang pig was deduced. The location of Met 55 Leu was next to the calcium binding site in PON1 structure. It had been demonstrated that the hydrolase activity of PON1-M55 decreased and related to the metabolism disorder in human patients, and the polymorphism of PON1 gene was correlated with the meat quality in pig. It suggested that the polymorphism at site 163/165 in PON1 gene might contribute to the improvement of meat quality in Guizhou indigenous pig breeds.

Key words: Guizhou indigenous pig breeds; paraoxonase 1 gene; polymorphism; three-dimension structure; meat quality

中图分类号:

S828

郑芳霞, 王嘉福, 冉雪琴. 贵州地方猪品种对氧磷脂酶1基因的多态性研究[J]. , 2013, (5): 144-149.

ZHENG Fang-xia, WANG Jia-fu, RAN Xue-qin. Study on Polymorphism of Paraoxonase 1 Gene in Guizhou Indigenous Pig Breeds[J]. , 2013, (5): 144-149.

参考文献

1 于碧莲,赵水平.对氧磷酶1与动脉粥样硬化的研究进展[J].中国动脉硬化杂志,2005,13(5):655~658.
2 李升,牛熙,冉雪琴,等.香猪对氧磷酯酶基因家族的克隆及序列分析[J].中国畜牧兽医,2012,39(6):164~168.
3 胡倩,王嘉福,冉雪琴.贵州地方猪种UCP3基因多态性与肉质性状的相关性分析[J].中国畜牧杂志,2004,44(13):1~4,45.
4 赵赞梅,王汉斌.对氧磷酶的某些研究进展[J].毒理学杂志,2005,19(1):64~66.
5 姬爱国,周正奎,张路培,等.牛PON1基因对生长和胴体性状的影响[J].畜牧兽医学报,2009,40(1):122~128.
6 梁红业,吴白燕,陈大方,等.新生儿基因CYP2E1 5'端RsaⅠ和PON2311位点多态性与早产的关系[J].遗传学报,2002,29(10):847~853.
7 Aydin S, Sahin I, Aydin S, et al. The past and present of paraoxonase enzyme: Its role in the cardiovascular system and some diseases[J]. J Med Biochem, 2012, 31: 161~173.
8 Ben-david M, Elias M, Filippi J J, et al. Catalytic versatility and backups in enzyme active sites: The case of serum paraoxonase 1[J]. J Mol Biol, 2012, 418(3~4): 181~196.
9 Eom S Y, Kim Y S, Lee C J, et al. Effects of intronic and exonic polymorphisms of paraoxonase 1 (PON1) gene on serum PON1 activity in a Korean population[J]. J Korean Med Sci, 2011, 26(6): 720~725.
10 Gupta N, Gill K D, Singh S. Paraoxonase 1 (PON1) activity, polymorphisms and coronary artery disease[M]. Croatia: Published by InTech, 2012.
11 Harel M, Aharoni A, Gaidukov L, et al. Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes[J]. Nat Struct Mol Biol, 2004, 11(5): 412~419.
12 Hegele R A. Paraoxonase genes and disease[J]. Ann Med, 1999, 31(3): 217~224.
13 Kaman D, Ilhan N, Metin K, et al. A preliminary study of human paraoxonase and PON1 L/M55-PON 1 Q/R 192 polymorphisms in Turkish patients with coronary artery disease[J]. Cell Biochem Funct, 2009, 27(2): 88~92.
14 Macharia M, Hassan M S, Blackhurst D, et al. The growing importance of PON1 in cardiovascular health: A review[J]. J Cardiovasc Med, 2012, 13(7): 443~453.
15 Mackness M I, Mackness B, Durington P N, et al. Paraoxonase and coronary heart disease[J]. Curr Opin Lipidol, 1998, 9(4): 319~324.
16 Mackness B, Mackness M I, Arrol S, et al. Effect of the human serum paraoxonase 55 and 192 genetic polymorphisms on the protection by high densitylipoprotein against low density lipoprotein oxidative modification[J]. FEBS Lett, 1998, 423(1): 57~60.
17 Martinelli N, Micaglio R, Consoli L, et al. Low levels of serum paraoxonase activities are characteristic of metabolic syndrome and may influence the metabolic- syndrome-related risk of coronary artery disease[J]. Exp Diabetes Res, 2012, doi:10.1155/2012/231502.
18 Mehdi M M, Rizvi S I. Human plasma paraoxonase 1 (PON1) arylesterase activity during aging: Correlation with susceptibility of LDL oxidation[J]. Arch Med Res, 2012, 43(6): 438~443.
19 Tabak O, Geligen R, Ciceki H, et al. Circulating levels of adiponectin, orexin-A, ghrelin and the antioxidant paraoxonase-1 in metabolic syndrome[J].Minerva Med, 2012, 103(4): 323~329.
20 Xie S, Li J, Chen Y, et al. Sequence identification, chromosomal mapping and tissue specific expression of the porcine paraoxonase 1 (PON1) gene[J]. Mol Biol Rep, 2010, 37(3): 1347~1353.

[1]	王泽平, 王飞飞, 张芮铭, 付言峰, 赵为民, 戴超辉, 程金花, 廖超, 李辉. 苏紫猪SLA-1基因多态性及其抗病潜能分析[J]. 中国畜牧兽医, 2023, 50(5): 1918-1927.
[2]	张蕊, 穆春宇, 汤青萍, 卜柱, 杨明军, 夏爱萍, 杨晓明. 鸽GnIH基因多态性及其与产蛋性状的关联分析[J]. 中国畜牧兽医, 2023, 50(4): 1412-1421.
[3]	李中波, 侯强红, 段德勇. 不同种群白纹伊蚊单倍型多态性、遗传分化及种系发育关系分析[J]. 中国畜牧兽医, 2023, 50(4): 1452-1460.
[4]	谭元成, 罗华伦, 张依裕, 杨酸, 王盈童, 王春源. 白羽王鸽MyoG基因多态性及其与体尺和肉质性状的关联分析[J]. 中国畜牧兽医, 2023, 50(4): 1461-1471.
[5]	刘泽林, 杨娟, 许铭洙, 张年, 熊琪, 刘静波, 陶虎. 山羊ZBP1基因多态性及与产羔性能的关联分析[J]. 中国畜牧兽医, 2023, 50(1): 161-173.
[6]	龙安炬, 杨酸, 艾锦新, 翁吉梅, 张依裕, 蔡惠芬, 罗卫星. 黔北麻羊MC4R基因多态性及其与生长性状的关联分析[J]. 中国畜牧兽医, 2023, 50(1): 198-209.
[7]	杨曼, 刘海, 张润, 胡紫平, 牛乃琪, 王立贤, 张龙超. 北京黑猪MYH3和MYH13基因多态性与肉质性状的关联分析[J]. 中国畜牧兽医, 2022, 49(9): 3428-3437.
[8]	徐昊祺, 许静漪, 胡丽蓉, 张帆, 罗汉鹏, 张海亮, 师睿, 李想, 刘林, 刘巧香, 郭刚, 王雅春. 中国荷斯坦牛PIK3CB基因多态性及其与繁殖和产奶性状的关联分析[J]. 中国畜牧兽医, 2022, 49(9): 3438-3452.
[9]	丁梦琴, 黑达西·巴哈提, 彭凤强, 胡雅丽, 沙尔山别克·阿不地力大. 拜城油鸡FSHR基因多态性及其与生产性能的关联分析[J]. 中国畜牧兽医, 2022, 49(8): 3044-3053.
[10]	周迪, 敖叶, 蒋桂荣, 赵忠海, 李俊, 杨蓉, 龚菲, 陈昌雪, 沈银, 李辉. 黔东南小香鸡MEF2C基因外显子多态性及其与体重、体尺性状的关联分析[J]. 中国畜牧兽医, 2022, 49(7): 2613-2621.
[11]	马彦茹, 于永生, 曹阳, 秦立红, 赵玉民, 吴健. 中国草原红牛PDK4基因多态性及其与肉质性状的关联分析[J]. 中国畜牧兽医, 2022, 49(6): 2186-2194.
[12]	柴志欣, 武志娟, 王吉坤, 张成福, 钟金城, 信金伟. CTGF、Gprin3基因多态性及其与牦牛体尺性状的关联分析[J]. 中国畜牧兽医, 2022, 49(6): 2216-2227.
[13]	阮涌, 陈祥, 代林刚, 黄家锦, 安冬伟, 邹启顺. F3代波杂山羊VNN1基因多态性及其与产羔数的关联分析[J]. 中国畜牧兽医, 2022, 49(4): 1384-1392.
[14]	张永浩, 肖逸, 郭丽君, 阿布都热合曼·吐尔逊, 王玉涛. 马可·波罗盘羊和5种家养绵羊MHC-DRB3基因第2外显子遗传多态性分析[J]. 中国畜牧兽医, 2022, 49(3): 962-972.
[15]	方钱海, 陈洪波, 毕延震. 猪重大育种价值基因及挖掘技术研究进展[J]. 中国畜牧兽医, 2022, 49(3): 992-1004.