›› 2012, Vol. 39 ›› Issue (3): 60-64.

• 生物技术 • 上一篇    下一篇

猪繁殖与呼吸综合征病毒变异株FS的分离鉴定与全基因组序列分析

李嘉彬1,2, 赵孟孟1,2, 李玉谷2, 马春全1   

  1. 1. 佛山科学技术学院生命科学学院,广东佛山 528231;2. 华南农业大学兽医学院,广东广州 510642
  • 收稿日期:1900-01-01 修回日期:2011-10-17 出版日期:2012-03-20 发布日期:2012-03-20
  • 通讯作者: 马春全

Isolation, Identification and Genomic Sequencing of FS Mutation Strain of Porcine Reproductive and Respiratory Syndrome Virus

LI Jia-bin1,2, ZHAO Meng-meng1,2, LI Yu-gu2, MA Chun-quan1   

  1. 1. College of Life Science,Foshan University,Foshan 528231,China;2. College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China
  • Received:1900-01-01 Revised:2011-10-17 Online:2012-03-20 Published:2012-03-20

摘要: 从一例疑似高致病性猪繁殖与呼吸综合征病例中分离到一株病毒,经全基因组测序分析可知此毒株在Nsp2部位发生了30个氨基酸的缺失,GP5蛋白相对保守。本毒株与VR-2332同源性达到88.7%,与欧洲型代表株LV株的核苷酸同源性为58.5%,与国内报道的JN-HS毒株相似性最大,核苷酸同源性达到98.8%。

关键词: 猪繁殖与呼吸综合征病毒; 分离鉴定; 全基因组; 序列分析

Abstract: A porcine reproductive and respiratory syndrome virus (PRRSV) strain isolated from visceral organs was designated as PRRSV-FS. The sequence analysis revealed that the isolated virus was a mutation strain as the Nsp2 gene existed amino acid deletion of 30 and the GP5 gene was conservative relatively. The genome of PRRSV FS strain showed 88.7%, 98.8%,58.5% of nucleotide indentity with ATCC VR-22332, JN-HS,Europena Lelystad virus respectively.

Key words: PRRSV; isolation and identification; genome; sequencing and analysis

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