影响Fat-1转基因水牛胚胎发育因素的研究

›› 2012, Vol. 39 ›› Issue (12): 121-124.

影响Fat-1转基因水牛胚胎发育因素的研究

王建, 庞春英, 邓廷贤, 杨春艳, 郑海英, 黄芬香, 杨炳壮, 梁贤威

中国农业科学院广西水牛研究所, 农业部(广西)水牛遗传繁育重点实验室,广西南宁 530001

收稿日期:2012-05-09 出版日期:2012-12-20 发布日期:2012-12-19
通讯作者: 梁贤威(1962-),男,研究员,研究方向:水牛繁殖生物技术和营养。E-mail:liangbri@126.com E-mail:liangbri@126.com
作者简介:王建(1983-),男,河南人,实习研究员,硕士,研究方向:动物繁殖生物技术。
基金资助:
桂科基2010GXNSFA013096;桂科攻1123005-1;"广西'特聘专家’专项经费资助"。

Study on Factors Affecting the Development of Fat-1 Buffalo Transgenic Embryo

WANG Jian, PANG Chun-ying, DENG Ting-xian, YANG Chun-yan, ZHENG Hai-ying, HUANG Fen-xiang, YANG Bing-zhuang, LIANG Xian-wei

Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture (Guangxi), Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China

Received:2012-05-09 Online:2012-12-20 Published:2012-12-19

摘要/Abstract

摘要： 本试验比较了水牛卵母细胞体外受精后注射外源DNA的时间(5~6、9~10、13~14、17~18、21~22 h)和外源DNA的浓度(10、50、100 μg/mL)对Fat-1转基因水牛胚胎的发育影响。结果显示,9~10 h组的胚胎表达率和囊胚表达率最高,其中胚胎表达率极显著高于5~6 h组,囊胚表达率极显著高于21~22 h组(P<0.01);在浓度试验中,50 μg/mL浓度组的胚胎表达率和囊胚表达率最高,且极显著高于100 μg/mL(P<0.01)。

关键词: 水牛; 转基因; Fat-1; 胚胎

Abstract: The effects of injecting timing after fertilization (5 to 6, 9 to 10, 13 to 14, 17 to 18, 21 to 22 h) and exogenous DNA concentrations (10, 50 and 100 μg/mL) on the development of Fat-1 transgenic embryo were investigated. The results showed that the EGFP expressing rate and blastocysts’ EGFP expressing rate of 9 to 10 h group were the highest, the EGFP expressing rate were significantly higher than 5 to 6 h group(P<0.01) and the blastocysts’ EGFP expressing rate significantly higher than 21 to 22 h group(P<0.01);in the concentration test, the EGFP expressing rate and blastocysts’ EGFP expressing rate of 50 μg/mL group were the highest, and significantly higher than 100 μg/mL(P<0.01).

Key words: buffalo; transgenic; Fat-1; embryos

中图分类号:

S813.1

王建, 庞春英, 邓廷贤, 杨春艳, 郑海英, 黄芬香, 杨炳壮, 梁贤威. 影响Fat-1转基因水牛胚胎发育因素的研究[J]. , 2012, 39(12): 121-124.

WANG Jian, PANG Chun-ying, DENG Ting-xian, YANG Chun-yan, ZHENG Hai-ying, HUANG Fen-xiang, YANG Bing-zhuang, LIANG Xian-wei. Study on Factors Affecting the Development of Fat-1 Buffalo Transgenic Embryo[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(12): 121-124.

参考文献

1 何泉,田小利,戴蕴平,等.MLC_2-糜酶融合基因转基因小鼠的建立(简报)[J].中国医学科学院学报,1997,1:80.
2 陈大元.受精生物学[M].北京:科学出版社,2000.
3 葛银林,陈志红,康兆彬,等. n-3脂肪酸脱氢酶在人乳腺癌细胞内的表达和对其生长的影响[J].肿瘤防治杂志,2002,9(5特):112~115.
4 Balhorn R. A model for the structure of chromatin in mammalian sperm[J]. The Journal of Cell Biology, 1982,93(2): 298~305.
5 Brinster R L, Chen H Y, Trumbauer M E, et al.Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs[J]. Proceedings of the National Academy of Sciences of the United States of America, 1985, 82(13): 4438~4442.
6 Hawthorne A B,Daneshmend T K,Hawkey C J. Treatment of ulcerative colitis with fish oil supplementation: A prospective 12-month randomized controlled trial[J]. Gut, 1992,33:922~928.
7 Hirabayashi M, Kato M, Ishikawa A, et al. Factors affecting production of transgenic rats by ICSI-mediated DNA transfer: Effects of sonication and freeze-thawing of spermatozoa, rat strains for sperm and oocyte donors, and different constructs of exogenous DNA[J]. Mol Reprod Dev, 2005, 70(4): 422~428.
8 Kang Z B,Ge Y L,Chen Z H,et al. Adenoviral gene transfer of Cacnorhabditis elegann-3 fatty acid desaturase optimizes fatty acid composition in mammalian cells[J]. Proceedings of the National Academy of Sciences of the United States of America,2001,98:4050~4054.
9 Page R L, Buller S P, Subramanian A, et al.Transgenesis in mice by cytoplasmic injection of polylysine/DNA mixtures[J]. Transgenic Research, 1995,4(6):353~360.
10 Rose D P, Connolly J M. Omega-3 fatty acids as cancer chemopreventive agents[J]. Pharmacol Ther,1999, 83(3):217~244.
11 Simopoulos A R. Importance of the ration of omega-6/omega-3 essential fatty acids evolutionary aspects[J]. World Review Nutrition Dietetics,2003,92:11~22.
12 Spychalla J P, Kinney A J, Browse J. Identification of an animal omega-3 fatty acid desaturase by heterologous expression in Arabidopsis[J].Proc Natl Acad Sci USA, 1997, 94(4):1142~1147.
13 Xu K P, Greve T. A detailed analysis of early events during in vitro fertilization of bovine follicular oocytes[J]. Reproduction, 1988,82(1): 127~134.

[1]	李志毅, 李洁, 陈楚雯, 农意, 王佳燕, 王孜, 吴锦波, 李志雄. 藏鸡胚胎不同发育阶段腿肌组织中miRNA的分析及鉴定[J]. 中国畜牧兽医, 2025, 52(4): 1681-1693.
[2]	徐光勇, 刘振田, 陈祥飞, 张美洁, 杜涛, 张俊波, 刘智辉, 邢雪松, 杜福良. 和牛OPU卵母细胞体外受精后胚胎发育潜能研究[J]. 中国畜牧兽医, 2025, 52(2): 730-739.
[3]	周芳廷. 水牛KLF15基因克隆、分子特征和组织差异表达分析[J]. 中国畜牧兽医, 2024, 51(9): 3715-3725.
[4]	孙慧敏, 范冰峰, 张旭林, 邵静, 刘理想, 许保增. 梅花鹿早期妊娠不同时期血液转录组分析[J]. 中国畜牧兽医, 2024, 51(9): 3892-3908.
[5]	郑湘民, 王玉琪, 徐冰洁, 孙朝阳, 高青山, 方南洙, 金庆国. 千金藤素对牛体外卵母细胞和早期胚胎抗凋亡能力的影响[J]. 中国畜牧兽医, 2024, 51(7): 2943-2952.
[6]	符彬彬, 王东升, 何凡, 李清春, 祁梦凡, 张化鹏, 黄涛. 猪miR-192靶向调控Wnt7a基因表达的研究[J]. 中国畜牧兽医, 2024, 51(6): 2300-2307.
[7]	黄丽清, 段安琴, 郑海英, 杨春艳, 尚江华. 水牛SFRP1基因序列分析、真核表达载体构建及组织表达分析[J]. 中国畜牧兽医, 2024, 51(5): 1807-1818.
[8]	莫显红, 陶庆华, 房义, 梁婧, 张佳音, 梁小航, 徐振军. 白血病抑制因子对牛卵母细胞体外发育能力的影响[J]. 中国畜牧兽医, 2024, 51(2): 462-469.
[9]	闫艳霞, 李紫聪, 董亚铮, 李政, 黄思秀. 基因修饰技术及其在动物育种和生物医药领域的应用[J]. 中国畜牧兽医, 2024, 51(2): 659-667.
[10]	罗安凤, 张玉清, 王昕昕, 杨彩侠, 解迪. Ola1对小鼠早期胚胎体外发育的影响[J]. 中国畜牧兽医, 2024, 51(11): 4871-4879.
[11]	徐媛媛, 郑海英, 杨春艳, 邓廷贤, 黄晨茜, 冯超, 陆杏蓉, 段安琴, 莫霞, 马小娅, 尚江华. 抑制BMP/Smad信号通路对水牛卵巢颗粒细胞生长和类固醇激素合成的影响[J]. 中国畜牧兽医, 2023, 50(6): 2354-2362.
[12]	肖鹏, 尚江华, 杨春艳, 李孟琪, 段安琴, 马小娅, 冯超, 黄晨茜, 张博, 周金陈, 韦科龙, 郑威, 郑海英. α-亚麻酸对水牛卵巢颗粒细胞体外培养的影响[J]. 中国畜牧兽医, 2023, 50(6): 2380-2387.
[13]	相德才, 李水英, 张斌, 张彦, 梁家充, 吕春荣, 洪琼花, 权国波, 吴国权. 胎牛血清在猪孤雌囊胚玻璃化冷冻后恢复培养中的作用研究[J]. 中国畜牧兽医, 2023, 50(2): 606-615.
[14]	徐茜, 杨宇泽, 郝海生, 杜卫华, 朱化彬, 杨柏高, 赵学明. 家畜卵母细胞和胚胎去脂方法研究进展[J]. 中国畜牧兽医, 2023, 50(2): 626-637.
[15]	梁莎莎, 于农淇, 卢瑛, 李厅厅, 鄢胜飞, 黄健, 谭正准, 黄荣春, 李辉, 潘伟军, 覃广胜. 基于GEO数据库筛选与水牛肌内脂肪沉积相关的候选基因及生物信息学分析[J]. 中国畜牧兽医, 2023, 50(11): 4359-4369.