影响Fat-1转基因水牛胚胎发育因素的研究

›› 2012, Vol. 39 ›› Issue (12): 121-124.

影响Fat-1转基因水牛胚胎发育因素的研究

王建, 庞春英, 邓廷贤, 杨春艳, 郑海英, 黄芬香, 杨炳壮, 梁贤威

中国农业科学院广西水牛研究所, 农业部(广西)水牛遗传繁育重点实验室,广西南宁 530001

收稿日期:2012-05-09 出版日期:2012-12-20 发布日期:2012-12-19
通讯作者: 梁贤威(1962-),男,研究员,研究方向:水牛繁殖生物技术和营养。E-mail:liangbri@126.com E-mail:liangbri@126.com
作者简介:王建(1983-),男,河南人,实习研究员,硕士,研究方向:动物繁殖生物技术。
基金资助:
桂科基2010GXNSFA013096;桂科攻1123005-1;"广西'特聘专家’专项经费资助"。

Study on Factors Affecting the Development of Fat-1 Buffalo Transgenic Embryo

WANG Jian, PANG Chun-ying, DENG Ting-xian, YANG Chun-yan, ZHENG Hai-ying, HUANG Fen-xiang, YANG Bing-zhuang, LIANG Xian-wei

Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture (Guangxi), Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China

Received:2012-05-09 Online:2012-12-20 Published:2012-12-19

摘要/Abstract

摘要： 本试验比较了水牛卵母细胞体外受精后注射外源DNA的时间(5~6、9~10、13~14、17~18、21~22 h)和外源DNA的浓度(10、50、100 μg/mL)对Fat-1转基因水牛胚胎的发育影响。结果显示,9~10 h组的胚胎表达率和囊胚表达率最高,其中胚胎表达率极显著高于5~6 h组,囊胚表达率极显著高于21~22 h组(P<0.01);在浓度试验中,50 μg/mL浓度组的胚胎表达率和囊胚表达率最高,且极显著高于100 μg/mL(P<0.01)。

关键词: 水牛; 转基因; Fat-1; 胚胎

Abstract: The effects of injecting timing after fertilization (5 to 6, 9 to 10, 13 to 14, 17 to 18, 21 to 22 h) and exogenous DNA concentrations (10, 50 and 100 μg/mL) on the development of Fat-1 transgenic embryo were investigated. The results showed that the EGFP expressing rate and blastocysts’ EGFP expressing rate of 9 to 10 h group were the highest, the EGFP expressing rate were significantly higher than 5 to 6 h group(P<0.01) and the blastocysts’ EGFP expressing rate significantly higher than 21 to 22 h group(P<0.01);in the concentration test, the EGFP expressing rate and blastocysts’ EGFP expressing rate of 50 μg/mL group were the highest, and significantly higher than 100 μg/mL(P<0.01).

Key words: buffalo; transgenic; Fat-1; embryos

中图分类号:

S813.1

王建, 庞春英, 邓廷贤, 杨春艳, 郑海英, 黄芬香, 杨炳壮, 梁贤威. 影响Fat-1转基因水牛胚胎发育因素的研究[J]. , 2012, 39(12): 121-124.

WANG Jian, PANG Chun-ying, DENG Ting-xian, YANG Chun-yan, ZHENG Hai-ying, HUANG Fen-xiang, YANG Bing-zhuang, LIANG Xian-wei. Study on Factors Affecting the Development of Fat-1 Buffalo Transgenic Embryo[J]. , 2012, 39(12): 121-124.

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