肉鸡胚胎发育过程中肝脏的组织学观察

›› 2012, Vol. 39 ›› Issue (11): 111-115.

肉鸡胚胎发育过程中肝脏的组织学观察

那威, 吴媛媛, 王宇祥, 李辉

东北农业大学动物科学技术学院,黑龙江哈尔滨 150030

收稿日期:2012-05-02 出版日期:2012-11-20 发布日期:2012-11-22
通讯作者: 李辉。E-mail:lihui645@hotmail.com E-mail:lihui645@hotmail.com
作者简介:那威(1986-),女,黑龙江人,硕士,研究方向:动物遗传育种与繁殖。
基金资助:
现代农业产业技术体系建设专项资金(CARS-42);国家重点基础研究发展计划(973计划)项目(2009CB941604);黑龙江省高等学校科技创新团队建设项目(2010td02)。

Histological Observations on the Broiler Liver Tissue during Embryonic Period

NA Wei, WU Yuan-yuan, WANG Yu-xiang, LI Hui

College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030,China

Received:2012-05-02 Online:2012-11-20 Published:2012-11-22

摘要/Abstract

摘要： 肝脏是家禽体内最大的消化腺,其发生发育与功能的行使紧密相连。本研究从组织学的角度观察肉鸡肝脏组织在胚胎发育过程中的特点,并比较高、低脂系间肝脏组织在发育过程中的区别。结果发现,肝叶体积随日龄的增长而增加;胚胎发育至12日龄时肝小叶结构基本发育完整;在胚胎发育后期,肝细胞内脂滴明显增加,且肝叶周边的肝细胞较肝叶中心的肝细胞增殖活动旺盛。而在整个胚胎发育过程中,两系间肝细胞内脂滴沉积并没有明显的区别。

关键词: 鸡胚; 肝脏; 组织学观察

Abstract: The liver is the largest digestive gland in poultry, and its growth and development are vital for playing functions. In the current study we observed the development of broiler liver tissue with the histological method, and compared the differences of the development of liver tissue between fat and lean lines during embryonic period. We found that the lobes of liver become bigger with developing, and the hepatic lobule structure was integrity when chicken embryo was 12-day-old. The hyperplastic and hypertrophic growth lipid droplets in liver cells significantly increased in the later stage of the embryogenesis, and the proliferation activity of liver cells located around was stronger than those which located in the center of the lobes of liver. During the whole embryonic period, there was no difference on the deposition of lipid droplets between the two lines.

Key words: chicken embryo; liver; histological observations

中图分类号:

S831.1

那威, 吴媛媛, 王宇祥, 李辉. 肉鸡胚胎发育过程中肝脏的组织学观察[J]. , 2012, 39(11): 111-115.

NA Wei, WU Yuan-yuan, WANG Yu-xiang, LI Hui. Histological Observations on the Broiler Liver Tissue during Embryonic Period[J]. , 2012, 39(11): 111-115.

参考文献

1 王凤华,戚飞,王磊,等.鸡胚肝脏发育过程中增殖细胞核抗原及核仁组织区的免疫组化观察[J].中国兽医杂志,2008(9):87~88.
2 沈霞芬.家畜组织学与胚胎学[M].第3版.北京:中国农业出版社,2001.
3 张德禄,俞诗源,刘世倩,等.红腹锦鸡肝脏的显微结构观察[J].西北师范大学学报:自然科学版,2002,38(2):61~63.
4 罗克.家禽解剖学与组织学[M].福州:福建科学技术出版社,1983.
5 程树军.肝脏组织切片观察[J].中国实验动物学杂志,1994(1):15~16.
6 廖望,付晶,白秀娟. 冷应激鹌鹑盲肠、直肠的超微组织学病理观察[J]. 中国畜牧兽医, 2012, 39(3): 174~181.
7 Calabotta D F, Cherry J A, Siegel P B, et al. Lipogenesis and lipolysis in fed and fasted chicks from high and low body weight lines[J]. Poultry Science,1985,64(4):700~704.
8 Douarin N M. An experimental analysis of liver development[J]. Med Biol,1975,53(6):427~455.
9 Evans A J. In vitro lipogenesis in the liver and adipose tissues of the female Aylesbury duck at different ages[J].Poultry Science,1972,13(6):595~602.
10 Freeman B M, Vince M A. Development of the avian embryo. A behavioural and physiological study[M]. London: Chapman and Hall,1974.
11 Goodridge A G. Lipolysis in vitro in adipose tissue from embryonic and growing chicks[J]. Am Physiological Society,1968,214(4):902~907.
12 Gouysse G, Couvelard A, et al. Relationship between vascular development and vascular differentiation during liver organogenesis in humans[J]. Journal of Hepatology,2002,37(6):730~740.
13 Shand J H, West D W, et al. The esterification of cholesterol in the liver of the chick embryo[J]. Biochimicaet Biophysica Acta,1994,1213(2):224~230.
14 Kanai M, Soji T, Sugawara E, et al. Participation of endodermal epithelial cells on the synthesis of plasma LDL and HDL in the chick yolk sac[J]. Microscopy Research and Technology,1996,35(4):340~348.
15 Liggins G C. Reproduction in mammals. Book 2: Embryonic and fetal development [M]. Cambridge: Cambridge University Press,1990.
16 Guo L, Sun B, Li H. Comparison of adipose tissue cellularity in chicken lines divergently selected for fatness[J]. Poultry Science, 2011, 90(9):2024~2034.
17 Noble R C, Cocchi M. Lipid metabolism and the neonatal chicken[J]. Progress in Lipid Research,1990,29(2):107~140.
18 Noble R C, Moore J H. The partition of lipids between the yolk and yolk-sac membrane during the development of the chick embryo[J]. Canadian Journal of Biochemistry,1967,45(6):949~958.
19 Romanoff A L. Biochemistry of the avian embryo. A quantitative analysis of prenatal development[M].New York:Interscience Publishers,1967.
20 Romanoff A L. The avian embryo. Structural and functional development[M]. New York: The Macmillan Company,1960.
21 Suksaweang S, Lin C M, Chuong C M. Morphogenesis of chicken liver: Identification of localized growth zones and the role of β-catenin/Wnt in size regulation[J]. Developmental Biology,2004,266(1):109~122.
22 Suckling K E, Stange E F. Role of acyl-CoA: Cholesterol acyltransferase in cellular choesterol metabolism[J]. Lipid Res,1985,26:647~671.

[1]	徐嘉辉, 谢嘉欣, 姜成坤, 张森, 姚华, 张华. 三维重建和3D打印技术在比格犬肝脏模型构建中的应用[J]. 中国畜牧兽医, 2023, 50(5): 2130-2138.
[2]	吴涛, 齐云霞, 刘伟, 朱洪龙, 黄冬维, 丁海生, 赵辉玲, 程广龙, 赵小伟. 基于蛋白质组学技术分析初乳和常乳摄入对初生犊牛肝脏蛋白表达的影响[J]. 中国畜牧兽医, 2023, 50(1): 37-45.
[3]	张润祥, 白玉婷, 张颖蕾, 刘可可, 王欣雨, 董胤余, 王晓丽. 绞股蓝多糖对D-半乳糖致衰老小鼠肝脏抗氧化能力的影响[J]. 中国畜牧兽医, 2022, 49(2): 462-470.
[4]	崔埕瑜, 马贲, 赵佳慧, 赵梓娟, 陈雨, 曹中赞, 栾新红. 脂联素及其受体激动剂在禽脂肪性肝病中的应用展望[J]. 中国畜牧兽医, 2022, 49(2): 738-745.
[5]	张园园, 田志辰, 孙雨佳, 张慧敏, 杨章平, 王聪, 曹柯, 毛永江, 李明勋. 补充牛磺酸对高脂饮食C57BL/6J小鼠糖脂代谢的影响[J]. 中国畜牧兽医, 2022, 49(12): 4604-4613.
[6]	安晓羽, 郝伟, 孙利婷, 杨丹妮, 常悦, 赵婷, 李康, 李喻瞳, 张智精, 杨慧娣. DHA对高脂饲粮诱导肝脏脂肪积累的预防作用[J]. 中国畜牧兽医, 2022, 49(1): 140-149.
[7]	严静, 王银龙, 王广泽, 张玥, 汪培嘉, 许小琴. 鸡白痢沙门氏菌鸡胚感染模型建立方法的筛选[J]. 中国畜牧兽医, 2021, 48(8): 3050-3057.
[8]	耿玉萌, 李淑颖, 卢春雨, 崔宇擎, 史万玉, 包永占. 丹参多糖抑制氟苯尼考致肉鸡肝脏损伤的分子机制研究[J]. 中国畜牧兽医, 2021, 48(5): 1849-1858.
[9]	崔淹鸽, 李京帅, 李银聚, 张春杰. 绿原酸对FAdV-4的抑制作用及其对鸡胚肝脏炎性因子和信号分子表达的影响[J]. 中国畜牧兽医, 2021, 48(12): 4718-4724.
[10]	朱根生, 夏苏干, 佘进进, 白玉妮, 邹辉, 顾建红, 袁燕, 刘学忠, 刘宗平, 卞建春. 白藜芦醇对玉米赤霉烯酮致小鼠肝脏氧化损伤及炎症的保护作用[J]. 中国畜牧兽医, 2021, 48(11): 4254-4261.
[11]	梁丽清, 黄鑫, 陈少锋, 杨增艳, 苏华珍, 卢苇, 王河. 桃金娘水提物抗肝脏纤维化作用的试验研究[J]. 中国畜牧兽医, 2021, 48(1): 367-374.
[12]	徐文文, 夏利, 孙甜甜, 邓继贤, 杨祝良, 杨秀荣. 过表达FOXL2基因对鸡胚性腺分化的影响[J]. 中国畜牧兽医, 2020, 47(9): 2857-2865.
[13]	袁婷, 郝华芳, 马丽娜, 陈胜利, 颜新敏, 吴娅琴, 蔡亚婷, 孙延鸣, 储岳峰. 绵羊肺炎支原体人工感染SPF鸡胚的研究[J]. 中国畜牧兽医, 2020, 47(9): 2988-2996.
[14]	董毅, 王倩, 孟禹璇, 朱哲坤, 田俊杰, 王蕾, 赵迪, 侯永清. 复合氨基酸对断奶仔猪肝脏能量状态、游离氨基酸含量及其相关基因表达的影响[J]. 中国畜牧兽医, 2020, 47(8): 2445-2453.
[15]	李欣, 赵中利, 吕阳, 柳俭强, 魏天, 金海国, 于永生. 视黄酸诱导鸡胚胎干细胞向雄性生殖细胞分化的研究[J]. 中国畜牧兽医, 2020, 47(7): 2142-2149.