绵羊繁殖性状相关基因的研究进展

摘要/Abstract

摘要： 绵羊的繁殖特性已成为绵羊生产的一个重要研究方向,对绵羊繁殖力进行遗传改良是提升中国绵羊生产水平的必由之路,母羊的年产羔能力主要受单胎产羔数和季节性发情的影响。本研究对影响产羔能力的排卵数和季节性发情相关基因及miRNA的研究进行了简要的综述,并对全基因组比较在繁殖性状相关QTL检测中的应用进行了展望。

关键词: 绵羊; 繁殖; 排卵; 季节发情; 全基因组

Abstract: Reproductive traits of sheep has became an important research area in sheep industry. Genetic improvement of sheep fecundity is the only way to improve sheep production levels in China.The annual ewe lambing ability is mainly affected by the impact of seasonal estrus and the litter size of single births. In this view, the author gives a brief review on ovulation and seasonal estrus related genes and miRNAs, and prospects the genome-wide comparison used in the detection of QTL about reproductive traits.

Key words: sheep; reproduction; ovulation; seasonal estrus; whole-genome

中图分类号:

S826

吕晓曼, 苏蕊, 张文广, 李金泉. 绵羊繁殖性状相关基因的研究进展[J]. , 2012, 39(10): 185-190.

LV Xiao-man, SU Rui, ZHANG Wen-guang, LI Jin-quan. Review on Genes Involved in Reproduction of Sheep[J]. , 2012, 39(10): 185-190.

参考文献

1 于成江,陈玉林,刘福元,等. 常年性和季节性发情绵羊品种HIOMT基因的PCR-SSCP分析[J]. 西北农林科技大学学报,2008,36(5):12~21.
2 王启贵,钟发刚,李辉,等. 绵羊BMPR-IB基因多态性与其产羔数的相关研究[J]. 草食家畜,2003(2):20~23.
3 王启贵,钟发刚,李辉,等. 绵羊产羔性状主效基因检测研究[J]. 遗传,2005, 27(1):80~84.
4 王根林,毛鑫智,Davis G H,等. DNA分析发现我国湖羊和小尾寒羊存在Booroola (FecB)多胎基因[J]. 南京农业大学学报,2003, 26(1):104~106.
5 冯涛,储明星,王西筠,等. 哺乳动物繁殖节律相关基因的研究进展[J]. 中国畜牧兽医,2010,37(7):89~92.
6 闫亚东,储明星,曾勇庆,等. 小尾寒羊和湖羊高繁殖力候选基因BMPR-IB的研究[J]. 农业生物技术学报, 2005,13(1):66~71.
7 张英杰,刘月琴,储明星. 小尾寒羊高繁殖力和常年发情内分泌机理的研究[J]. 畜牧兽医学报, 2001,32(6):510~516.
8 杜立新,曹顶国. 小尾寒羊微卫星与RAPD标记的研究[J]. 遗传学报,2003,30(11):1041~1044.
9 汪兴生,夏银,尚平,等. 褪黑激素RIA方法的建立及其在探索湖羊四季发情机理上的应用[J]. 中国兽医学报,2001,5(3):287~290.
10 周琦,王文. DNA水平自然选择作用的检测[J]. 动物学研究,2004,25(1):73~80.
11 姜云良,吴常信. Booroola Merino绵羊多胎基因Fec的研究进展[J]. 中国畜牧杂志,1999(4):51~53.
12 柳淑芳,姜运良,杜立新. BMPR-IB和BMP15基因作为小尾寒羊多胎性能候选基因的研究[J]. 遗传学报,2003, 30(8):755~760.
13 殷子惠,姜运良,樊新忠,等. 小尾寒羊BMPR-IB基因的多态性、效应及连锁分析[J]. 畜牧兽医学报,2006,37(5):510~513.
14 储明星,桑林华,王金玉,等. 小尾寒羊高繁殖力候选基因BMP15和GDF9的研究[J]. 遗传学报,2005, 32(1):38~45.
15 Abdennebi L, Monget P, Pisselet C, et al. Comparative expression of luteinizing hormone and follicle-stimulating hormone receptors in ovarian follicles from high and low prolific sheep breeds[J]. Biol Reprod, 1999, 60(4):845~854.
16 Abdulkhaliq A M, Harvey W R, Parker C F. Genetic parameters for ewe productivity traits in the Columbia, Suffolk and Targhee breeds[J]. J Anim Sci, 1989,67(12):3250~3257.
17 Bartlewski P M, Beard A P, Cook S J, et al. Ovarian antral follicular dynamics and their relationships with endocrine variables throughout the oestrous cycle in breeds of sheep differing in prolificacy[J]. J Reprod Fertil, 1999, 115(1):111~124.
18 Bindon B M, Findlay J K, Piper L R. Plasma FSH and LH in prepubertal Booroola ewe lambs[J]. Aust J Biol Sci, 1985, 38(2):215~220.
19 Bodensteiner K J, Clay C M, Moeller C L, et al. Molecular cloning of the ovine growth differentiation factor 9 gene and expression of growth differentiation factor 9 in ovine and bovine ovaries[J]. Biol Reprod, 1999,60(2):381~386.
20 Bodensteiner K J, McNatty K P, Clay C M, et al. Expression of growth and differentiation factor 9 in the ovaries of fetal sheep homozygous or heterozygous for the Inverdale prolificacy gene (FecXI)[J]. Biol Reprod, 2000,62(6):1479~1485.
21 Bodin L, Di Pasquale E,Fabre S,et al.A novel mutation in the BMP15 gene causing defective protein secretion is associated with both increased ovulation rate and sterility in Lacaune sheep[J]. Endocrinology, 2007, 148(1):393~400.
22 Campion C E, Turzillo A M, Clay C M. The gene encoding the ovine gonadotropin-releasing hormone (GnRH) receptor: Cloning and initial characterization[J]. Gene, 1996, 170(2):277~280.
23 Rubin C J, Zody M C, Eriksson J, et al. Whole-genome resequencing reveals loci under selection during chicken domestication[J]. Nature, 2010, 464(7288):587~591.
24 Casas E, Freking B A,Leymaster K A. Evaluation of Dorset, Finnsheep, Romanov, Texel, and Montadale breeds of sheep: Ⅱ. Reproduction of F1 ewes in fall mating seasons[J]. J Anim Sci, 2004,82(5):1280~1289.
25 Chu M X,Cheng D X,Liu W Z,et al. Association between melatonin receptor 1A gene and expression of reproductive seasonality in sheep[J]. Asian-Australasian Journal of Animal Sciences,2006,19(8):1079~1084
26 Chu M X,Liu Z H,Jiao C L,et al. Mutations in BMPR-IB and BMP-15 genes are associated with litter size in Small Tailed Han sheep (Ovis aries)[J]. J Anim Sci, 2007, 85(3):598~603.
27 Hoggartl C J, Whittaker J C, De Iorio M, et al. Simultaneous analysis of all SNPs in genome-wide and re-sequencing association studies[J]. Plos Genetics, 2008,4(7):e1000130.
28 Dalrymple B P, Kirkness E F, Nefedov M,et al. Using comparative genomics to reorder the human genome sequence into a virtual sheep genome[J]. Genome Biology, 2007, 8(7):R152.
29 Notter D R, Cockett N E. Opportunities for detecetion and use of QTL influencing seasonal reproduction in sheep[J]. GSE,2005,37(S1):S39~S53.
30 Davis G H, Balakrishnan L, Ross I K, et al. Investigation of the Booroola (FecB) and Inverdale (FecXI) mutations in 21 prolific breeds and strains of sheep sampled in 13 countries[J]. Anim Reprod Sci, 2006, 92(1~2):87~96.
31 Davis G H, Galloway S M, Ross I K, et al. DNA tests in prolific sheep from eight countries provide new evidence on origin of the Booroola (FecB) mutation[J]. Biol Reprod, 2002, 66(6):1869~1874.
32 Dong J W, Albertini D F, Nishimori K, et al. Growth differentiation factor 9 is required during early ovarian folliculogenesis[J]. Nature, 1996,383(6600):531~535.
33 Dunlap J C. Molecular bases for circadian clocks[J]. Cell,1999,96(2):271~290.
34 Fahmy M H. Development of DLS breed of sheep: Genetic and phenotypic parameters of date of lambing and litter size[J]. Can J Anim Sci,1990(70):771~778.
35 Fiedler S D, Carletti M Z, Hong X, et al. Hormonal regulation of microRNA expression in periovulatory mouse mural granulosa cells[J]. Biol Reprod, 2008, 79(6):1030~1037.
36 Galloway S M, McNatty K P, Cambridge L M, et al. Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner[J]. Nature Genetics, 2000, 25(3):279~283.
37 Guan F, Liu S R, Shi G Q, et al. Polymorphism of FecB gene in nine sheep breeds or strains and its effects on litter size, lamb growth and development[J]. Acta Genetica Sinica, 2006, 33(2):117~124.
38 Hanrahan J P, Gregan S M, Mulsant P, et al. Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries)[J]. Biol Reprod, 2004, 70(4):900~909.
39 Hediger R, Johnson S E, Hetzel D J S. Localization of the β-subunit of follicle stimulating hormone in cattle and sheep by in situ hybridization[J]. Anim Genet, 1991, 22:237~244.
40 Jia C L, Li N, Zhao X B, et al. Association of single nucleotide polymorphisms in exon 6 region of BMPR-IB gene with litter size traits in sheep[J]. Asian-Australasian Journal of Animal Sciences, 2005, 18(10):1375~1378.
41 Jiang Z H, Gibson J P, Archibald A L, et al. The porcine gonadotropin-releasing hormone receptor gene (GNRHR): Genomic organization, polymorphisms, and association with the number of corpora lutea[J]. Genome, 2001, 44:7~12.
42 Kennaway D J,Boden M J,Voultsios A. Reproductive performance in female clock Delta19 mutant mice[J]. Reproduction Fertility and Development,2005,16(8):801~810.
43 Kumar S, Mishra A K, Kolte A P, et al. Screening for Booroola (FecB) and Galway (FecXG) mutations in Indian sheep[J]. Small Ruminant Research, 2008, 80(1~3):57~61.
44 Lincoln G A, Andersson H, Loudon A. Clock genes in calendar cells as the basis of annual timekeeping in mammals-a unifyin hypothesis[J]. J Endocrinol,2003,179(1):1~13.
45 Lee R C, Feinbaum R L, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14[J]. Cell,1993,75 (5): 843~854.
46 Martinez-Royo A, Jurado J J, Smulders J P, et al. A deletion in the bone morphogenetic protein 15 gene causes sterility and increased prolificacy in Rasa Aragonesa sheep[J]. Animal Genetics, 2008, 39(3):294~297.
47 Malpaux B, Thiery J C, Chemineau P. Melatonin and the seasonal control of reproduction[J]. Reprod Nutr Dev, 1999, 39(3): 355~366.
48 Monteagudo L V, Ponz R, Tejedor M T, et al. A 17 bp deletion in the bone morphogenetic protein 15 (BMP15) gene is associated to increased prolificacy in the Rasa Aragonesa sheep breed[J]. Animal Reproduction Science, 2009, 110(1~2):139~146.
49 Montgomery G W, Penty J M, Lord E A, et al. The gonadotrophin-releasing hormone receptor maps to sheep chromosome 6 outside of the region of the FecB locus[J]. Mammalian Genome, 1995, 6(6):436~438.
50 Montgomery G W, Tate M L, Henry H M, et al. The follicle-stimulating hormone receptor and luteinizing hormone receptor genes are closely linked in sheep and deer[J]. Journal of Molecular Endocrinology, 1995, 15:259~265.
51 Moore R K, Otsuka F, Shimasaki S. Molecular basis of bone morphogenetic protein-15 signaling in granulosa cells[J]. J Biol Chem, 2003, 278(1):304~310.
52 Moore R K, Shimasaki S. Molecular biology and physiological role of the oocyte factor, BMP-15[J]. Mol Cell Endocrinol, 2005, 234(1~2):67~73.
53 Mulsant P, Lecerf F, Fabre S, et al. Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Merino ewes[J]. Proc Natl Acad Sci USA, 2001, 98(9):5104~5109.
54 Nagaraja A K, Andreu-Vieyra C, Franco H L, et al. Deletion of dicerin somatic cells of the female reproductive tract causes sterility[J]. Mol Endocrinol, 2008, 22(10):2336~2352.
55 Otsuka F, Shimasaki S. A negative feedback system between oocyte bone morphogenetic protein 15 and granulose cell kit ligand: Its role in regulating granulose cell mitosis[J]. Proc Natl Acad Sci USA, 2002, 99(12):8060~8065.
56 Otsuka F, Yamamoto S, Erickson G F, et al. Bone morphogenetic protein-15 inhibits follicle-stimulating hormone (FSH) action by suppressing FSH receptor expression[J]. J Biol Chem, 2001, 276(14):11387~11392.
57 Penty J M, Lord E A, Dodds K G, et al. Linkage of LHB and MAG to GPI on sheep chromosome 14. Mammalian Genome, 1995, 6:299~300.
58 Pan Q, Luo X, et al .The expression profile of micro-RNA in endometrium and endometriosis and the influence of ovarian steroids on their expression[J]. Molecular Human Reproduction, 2007,13(11):797~806.
59 Sadighi M, Bodensteiner K J, Beattie A E, et al. Genetic mapping of ovine growth differentiation factor 9 (GDF9) to sheep chromosome 5[J]. Anim Genet, 2002, 33(3):244~245.
60 Smith J F, Johnson D L, Reid T C. Genetic parameters for performance of flocks selected for advanced lambing date, Proc. New Zealand Soc[J]. Anim Prod, 1992(52):50.
61 Sonjaya H, Driancourt M A. FSH concentrations and sensitivity to feedback in infant lambs from breeds differing in prolificacy[J]. J Reprod Fertil, 1989, 85(2):461~469.
62 Souza C J H, MacDougall C, Campbell B K, et al. The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type IB (BMPR-IB) gene[J]. J Endocrinol, 2001, 169(2):R1~R6.
63 Sirotkin A V, Ovcharenko D, Grossmann R, et al. Identification of microRNAs controlling human ovarian cell steroidogenesis via a genome-scale screen[J]. Cell Physiol, 2009, 219(2):415~420.
64 Thrift F A, Dutt R H, Woolfolk P G. Phenotypic response and time trends to date of birth selection in Southdown sheep[J]. J Anim Sci, 1971,(33):1216~1223.
65 Wilson T, Wu X Y, Juengel J L, et al. Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granulosa cells. Biol Reprod, 2001, 64(4):1225~1235.

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