›› 2011, Vol. 38 ›› Issue (5): 158-160.

• 疾病防治 • 上一篇    下一篇

中国沿海养殖牡蛎尼氏单孢子虫感染情况调查

王宗祥1,2, 吴绍强2, 杨晓野1   

  1. 1. 内蒙古农业大学兽医学院, 内蒙古呼和浩特 010018;2. 中国检验检疫科学研究院, 北京 100029
  • 收稿日期:2010-10-18 修回日期:1900-01-01 出版日期:2011-05-20 发布日期:2011-05-20
  • 通讯作者: 吴绍强

Investigation of Haplosporidium nelsoni Infection in Breeding Oyster of China Bays

WANG Zong-xiang1,2, WU Shao-qiang2, YANG Xiao-ye1   

  1. 1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;2. Chinese Academy of Inspection and Quarantine, Beijing 100029, China
  • Received:2010-10-18 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20

摘要: 单孢子虫病是严重危害世界贝类养殖业的主要疫病之一。本研究选取中国沿海养殖的牡蛎作为研究对象,采用OIE推荐的PCR方法结合测序技术,对采自中国沿海4个监测点养殖牡蛎的感染情况进行调查,结果表明,这些监测点养殖的牡蛎均不同程度感染了单孢子虫。经PCR测序及序列分析表明感染种类均为尼尔森单孢子虫(Haplosporidium nelsoni)。

关键词: 单孢子虫; PCR; 测序; 感染情况调查; 序列分析

Abstract: Haplosporidiosis is a serious mollusc disease caused by parasitic Haplosporidium. The Haplosporidium-specific PCR detecting method, recommanded by World Animal Health Organization (OIE), was adopted to investigate Haplosporidiosis prevalence of oyster in three sampling sites of China east bays. Results showed that there was Haplosporidium prevalence in all the four sites in different degree. Through combining with DNA sequencing and sequence analysis, the infection species was identified to be Haplosporidium nelsoni.

Key words: Haplosporidium; PCR; sequencing; prevalence; sequence analysis 

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