›› 2011, Vol. 38 ›› Issue (4): 132-136.

• 遗传繁育 • 上一篇    下一篇

绒山羊11号染色体7个微卫星标记的连锁图谱的构建

王敏1,2, 赖双英1,2, 乔峰3, 李金泉2, 赵艳红2, 王志新1,2, 张文广2, 汪洋4, 徐磊1,2, 李浛5   

  1. 1. 内蒙古农业大学动物科学学院,内蒙古呼和浩特 010018;2. 内蒙古自治区动物遗传育种与繁殖重点实验室,内蒙古呼和浩特 010018;3. 内蒙古白绒山羊种羊场,内蒙古鄂尔多斯 017000;4. 中国兽医药品监察所,北京 100081;5. 北京市兽药监察所,北京 100107
  • 收稿日期:1900-01-01 修回日期:2011-02-28 出版日期:2011-04-20 发布日期:2011-04-20
  • 通讯作者: 赖双英

A Linkage Map of 7 Microsatellite Markers in the 11th Chromosome of Cashmere Goats

WANG Min1,2, LAI Shuang-ying1,2, QIAO Feng3, LI Jin-quan2, ZHAO Yan-hong2, WANG Zhi-xin1,2, ZHANG Wen-guang2, WANG Yang4, XU Lei1,2, LI Han5   

  1. 1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;2. Inner Mongolia Autonomous Region Key Laboratory of Animal Genetics,Breeding and Reproduction, Hohhot 010018, China;3. Inner Mongolia Cashmere Goat Breeding Farm,Ordos 017000, China;4. China Institute of Veterinary Drug Control,Beijing 100081, China;5. Beijing Institute of Veterinary Drug Control,Beijing 100107, China
  • Received:1900-01-01 Revised:2011-02-28 Online:2011-04-20 Published:2011-04-20

摘要: 本试验利用内蒙古白绒山羊5个家系中的632个个体,用11号染色体上的7个微卫星标记,构建绒山羊11号染色体遗传连锁图。结果表明,7个标记的等位基因数变化范围为8~14,杂合度在0.5886~0.9348之间,平均杂合度为0.8612,各标记的多态信息含量在0.7712~0.8990之间,平均多态信息含量为0.8472。构建的遗传连锁图总长度127.7 cM,其中标记ILSTS028与INRA108间距最大,为41.2 cM;ILSTS049和INRA131间距最小,为5.1 cM。

关键词: 绒山羊; 微卫星标记; 11号染色体; 遗传图谱

Abstract: The 7 microsatellite markers in the 11th chromosome were used to construct a linkage map of Cashmere goats with a 632 samples of 5 half-sib pedigrees after paternity testing. The results showed that the allele numbers of the 7 microsatellite markers varied from 8 to 14. The lowest and the highest values of the heterozygosity were 0.5886 and 0.9348, respectively, and the average value of it was 0.8612, indicating abundant genetic diversity in Inner Monglian Cashmere goats. Moreover, the polymorphism information content (PIC) value of 7 markers was from 0.7712 to 0.8990, and the average one was 0.8472. The length of the linkage map of the 11th chromosome was 127.7 cM. The nearest genetic distance was 5.1 cM and its location was between ILSTS049 and INRA131, however, the farest one was 41.2 cM and lay in ILSTS028 and INRA108.

Key words: Cashmere goat; microsatellite markers; 11th chromosomes; linkage map

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