›› 2011, Vol. 38 ›› Issue (3): 163-166.

• 疾病防治 • 上一篇    下一篇

高效液相色谱荧光检测法检测鸡肉中甲砜霉素残留

谢恺舟1，徐东1，陈书琴1，谢星2，黄玉萍1，贾龙飞1，郭辉生1，王金玉1   

  1. （1.扬州大学动物科学与技术学院，江苏省动物遗传繁育与分子设计重点实验室，江苏扬州 225009； 2.扬州大学兽医学院，江苏扬州 225009)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-03-20 发布日期:2011-03-20

Determination of Residues of Thiamphenicol in Chicken Muscle by HPLC with Fluorescence Detection

XIE Kai-zhou1，XU Dong1，CHEN Shu-qin1，XIE Xing2，HUANG Yu-ping1，JIA Long-fei1，GUO Hui-sheng1，WANG Jin-yu1   

  1. (1.Key Laboratory for Animal Genetic，Breeding，Reproduction and Molecular Design of Jiangsu Province，College of Animal Science and Technology，Yangzhou University,Yangzhou 225009，China；2.College of Veterinary Medicine，Yangzhou University，Yangzhou 225009，China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-03-20 Published:2011-03-20

摘要： 研究建立了用高效液相色谱荧光法检测鸡肉中甲砜霉素残留的方法。鸡肉经丙酮、二氯甲烷提取，饱和正己烷脱脂，氮吹仪吹干浓缩后，以乙腈―磷酸二氢钠溶液（0.01 mol/L，含0.005 mol/L十二烷基硫酸钠和0.1%三乙胺)（32∶68，V/V）为流动相，流速为1.0 mL/min，荧光检测激发波长为225 nm，发射波长为290 nm。甲砜霉素在0.01～1.75 mg/L浓度范围内，本方法线性关系良好，相关系数为0.9991。当甲砜霉素添加水平为5～500 μg/kg时，该方法平均回收率为79.49%～89.71%，相对标准偏差为4.96%～9.61%；日内相对标准偏差为5.68%～7.47%；日间相对标准偏差为8.89%～11.32%。甲砜霉素检测限为1.5 μg/kg（S/N＝3），定量限5 μg/kg（S/N＝10）。所建立的方法简便、快速、灵敏度高，适用于鸡肉中甲砜霉素残留的高灵敏度检测。

关键词: 高效液相色谱; 荧光检测法; 甲砜霉素; 鸡肉; 残留

Abstract: A high performance liquid chromatography with fluorescence detection(HPLC-FLD) method was built for determination of residues of thiamphenicol(TAP) in chicken muscle. TAP was extracted with acetone and dichloromethane from chicken muscle. Extract solution was degreased with n-hexane and evaporated with nitrogen evaporator，the mobile phase was acetonitrile-sodium dihydrogen phosphate solution(0.01 mol/L，containing 0.005 mol/L SDS and 0.1% triethylamine)(32∶68)，the flow rate was 1.0 mL/min. The fluorescence detection was with an excitation wavelength of 225 nm and an emission wavelength of 290 nm. The data showed that the linear relationship of TAP was nice (r＝0.9991) in 0.01 mg/L to 1.75 mg/L concentration range. At spiked level 5 to 500 μg/kg for TAP，the average recovery ranged from 79.49% to 89.71%，relative standard deviation(RSD) ranged from 4.96% to 9.61%，the within-day RSD ranged from 5.68% to 7.47%，the between day RSD ranged from 8.89% to 11.32%. The lowest detection limit of TAP was 1.5 μg/kg(S/N＝3) and the lowest quantitative limit of TAP was 5 μg/kg (S/N＝10). This HPLC-FLD method is simple，rapid，high sensitivity and suitable for high sensitivity determination of TAP in chicken muscle.

Key words: high performance liquid chromatography（HPLC）; fluorescence detection; thiamphenicol（TAP）; chicken muscle; residues

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