›› 2011, Vol. 38 ›› Issue (10): 106-112.

• 遗传繁育 • 上一篇    下一篇

9个绵羊品种微卫星DNA遗传多样性分析

娄渊根1,2, 刘丑生2, 刘建学1, 刘刚2, 韩旭2   

  1. 1. 河南科技大学食品与生物工程学院,河南洛阳 471003;2. 全国畜牧总站畜禽牧草种质资源保存利用中心,北京 100193
  • 收稿日期:2011-02-16 修回日期:1900-01-01 出版日期:2011-10-20 发布日期:2011-10-20
  • 通讯作者: 刘丑生

Genetic Diversity of Microsatellite DNA in Nine Sheep Breeds

LOU Yuan-gen1,2, LIU Chou-sheng2, LIU Jian-xue1, LIU Gang2, HAN Xu2   

  1. 1. College of Food & Bioengineering,Henan University of Science and Technology,Luoyang 471003,China;2. National Center for Preservation and Utilization of Genetic Resources of Domestic Animals and Forages, National Animal Husbandry and Veterinary Service, Beijing 100193,China
  • Received:2011-02-16 Revised:1900-01-01 Online:2011-10-20 Published:2011-10-20

摘要: 采用FAO-ISGA联合推荐的10对微卫星引物,结合荧光标记PCR技术,分析了中国9个地方绵品种的遗传多样性。结果表明,10个微卫星座位上共检测到153个等位基因,有效等位基因数在2.3064~13.7632之间,位点多态信息含量和观察杂合度为0.5395~0.8559和0.3972~0.8475,位点的遗传分化系数在0.042~0.149之间。Structure遗传结构关系、DA/UPGMA聚类图、MVSP聚类分析图三者的结论基本相吻合,与绵羊的起源进化、亲缘关系、地理分布相一致。

关键词: 绵羊; 微卫星; 遗传多样性

Abstract: The genetic diversity of nine Chinese indigenous sheep breeds were analyzed with fluorescence PCR using 10 microsatellite markers recommended by the FAO and ISGA. The results showed that 153 alleles were detected on 10 microsatellite loci, the effective number of which ranged from 2.3064 to 13.7632,and the loci polymorphism information content and heterozygosity varied from 0.5395 to 0.8559 and from 0.3972 to 0.8475, with locus genetic differentiation in the range from 0.042 to 0.149. The conclusion of the genetic structure relationship and DA / UPGMA dendrogram and MVSP cluster analysis diagram was basically consistent with sheep origin and evolution and genetic relationship and geographic distribution.

Key words: sheep; microsatellite; genetic diversity

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