›› 2011, Vol. 38 ›› Issue (1): 39-41.

• 生理生化 • 上一篇    下一篇

生大豆对小鼠消化道腺体氧自由基水平的影响

谷春梅1,2,施用晖2,乐国伟2   

  1. (1.吉林农业大学食品科学与工程学院,吉林长春 130118;2.江南大学食品科学与技术国家重点实验室,江南大学食品学院食品营养与安全研究所,江苏无锡 214122)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-01 发布日期:2011-01-01
  • 通讯作者: 乐国伟

Effect of Raw Soybean on Oxygen Free Radical Level of Digestive Tract and Pancreas in Mice

GU Chun-mei1,2, SHI Yong-hui2, LE Guo-wei2
  

  1. (1. School of Food Science and Engineering,Jilin Agricultural University,Changchun 130118, China;2. State Key Laboratory of Food Science and Technology, Research Institute of Food Nutrition and Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-01 Published:2011-01-01
  • Contact: LE Guo-wei

摘要: 为探讨生大豆对小鼠消化道腺体氧自由基水平的影响,试验选用30只C57BL/6J雄性小鼠,按体重随机分为正常对照组、生大豆组和抗氧化剂组3组,〖JP2〗饲养2周后屠宰,取十二指肠、肝脏和胰腺进行检测。结果表明,与对照组相比,生大豆能显著增加小鼠十二指肠、肝脏和胰腺的MDA含量和iNOS活性,显著降低SOD、GSH、T-AOC等抗氧化水平(P<0.05)。添加半胱胺后,氧化和抗氧化不平衡状态被改善。因此,生大豆日粮能增加小鼠消化道腺体氧自由基水平,使其处于氧化应激状态。

关键词: 生大豆; 抗营养因子; 氧自由基; 消化道; 腺体; 小鼠

Abstract: To study the effects of raw soybean on oxygen free radical level of digestive tract and pancreas in mice, thirty male C57BL/6J mice were randomly divided into control, raw soybean(RS) and cysteamine groups(CS). Each group was fed for 2 weeks, then duodenum, liver, pancreas samples were collected to examine the oxidative and antioxidative capability. The results showed that compared with control mice, MDA content and iNOS activity in duodenum, liver and pancreas of mice fed raw soybean were significantly increased, but levels of SOD, GSH and so on were significantly decreased. Imbalance state of oxidant and antioxidant was improved by cysteamine. These data demonstrated that raw soybean diet could significantly increase oxygen free radical level of digestive tract and pancreas in mice, and induce oxidative stress.

Key words: raw soybean; antinutritional factors; oxygen free radical; digestive tract; pancreas; mice

中图分类号: