›› 2011, Vol. 38 ›› Issue (1): 36-38.

• 生理生化 • 上一篇    下一篇

产蛋白酶和纤维素酶纳豆芽孢杆菌（B.Subtilis Natto）的筛选及其生物特性研究

奚晓琦1，2，王加启2，卜登攀2，孙妍2，周凌云2，魏宏阳2   

  1. （1.东北农业大学动物科学与技术学院，黑龙江哈尔滨 150030；2.中国农业科学院北京畜牧兽医研究所，动物营养学国家重点实验室，北京 100193）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-01 发布日期:2011-01-01
  • 通讯作者: 王加启

Screening of Protease and Cellulose Producing Bacillus Subtilis Natto Strains and their Biological Characteristics

XI Xiao-qi1，2，WANG Jia-qi2，BU Deng-pan2，SUN yan2，ZHOU ling-yun2，WEI Hong-yang2   

  1. （1.College of Animal Science and Technology， Northeast Agricultural University,Harbin 150030, China;2.State Key Laboratory of Animal Nutrition,Institute of Animal Sciences，Chinese Academic of Agricultural Sciences,Beijing 100193,China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-01 Published:2011-01-01
  • Contact: WANG Jia-qi

摘要： 采用酪蛋白平板和羧甲基纤维素钠（CMC-Na）平板初筛法，分别从北京、大连、日本3个产地的纳豆食品中分离筛选到10株产蛋白酶菌株（NY-1～NY-10）和10株产纤维素酶菌株（NS-1～NS-10）。经酶活力测定NY-1为蛋白酶活力最高菌株，NS-1为纤维素酶活力最高菌株。通过2株菌的生长曲线、芽孢形成曲线、产酶动态变化曲线和酶活力传代稳定性试验，得出NY-1的最佳种龄为16 h，最佳芽孢收获时间为28 h，芽孢量的对数值为(6.92±0.047)、蛋白酶产酶高峰时间为18 h，酶活力为（44.12±1.48） U/mL，；NS-1的最佳种龄为14 h，最佳芽孢收获时间为28 h，芽孢量的对数值为(8.41±0.0060)，纤维素酶产酶高峰时间为40 h，酶活力为（60.94±1.22） U/mL，蛋白酶和纤维素酶酶活力在7代内保持稳定。

关键词: 纳豆芽孢杆菌; 生长曲线; 芽孢; 蛋白酶; 纤维素酶; 筛选; 传代

Abstract: Using casein plate and carboxymethylcellulose sodium（CMC-Na） plate screening method, ten protease activity producing Bacillus Subtilis Natto strains（NY-1 to NY-10） and ten cellulose producing Bacillus Subtilis Natto strains（NS-1 to NS-10） were screened from Beijing, Dalian and Japanese Natto. NY-1 and NS-1 were selected from the examined strains for their highest protease activity and highest cellulose activity, respectively. NY-1 and NS-1 were studied with respect to their biological characteristics, including growth curves, sporulation curve, dynamic changes of enzyme activity and passage stability. The experimental results demonstrated that for strain NY-1 the best culture time was 16 h， the maximum logarithm of spores were (6.92±0.047) at 28 h, the maximum protease activity was （44.12±1.48） U/mL at 18 h ;for strain NS-1 the best culture time was 14 h， the maximum logarithm of spores was (8.41±0.0060) at 28 h, the maximum cellulose activity was （60.94±1.22） U/mL at 40 h. Protease activity of NY-1 and cellulose activity of NS-1 will keep a good passage stability within seven generations.

Key words: Bacillus Subtilis Natto; growth curve; spore; protease; screening; passaging

中图分类号: 

京ICP备10030713号-19
版权所有 © 2019 中国农业科学院北京畜牧兽医研究所
地址：北京市海淀区圆明园西路农大南路中国农业科学院北京畜牧兽医研究所　邮编：100193　
电话：010-62811226，62810371，62816020　E-mail：zgxmsy@caas.cn
本系统由北京玛格泰克科技发展有限公司设计开发