›› 2010, Vol. 37 ›› Issue (8): 177-180.

• 疾病防治 • 上一篇    下一篇

山羊中绵羊肺炎支原体的分离及鉴定

杨发龙,王华,岳华,王永   

  1. (西南民族大学生命科学与技术学院,成都 610041)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-08-20 发布日期:2010-08-20
  • 通讯作者: 王永

Isolation and Identification of Mycoplasma ovipneumoniae in Goat

YANG Fa-long, WANG Hua, YUE Hua, WANG Yong   

  1. (College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20
  • Contact: WANG Yong

摘要: 从四川省乐至县发生胸膜肺炎性传染病的山羊群中采集12个鼻拭子及4个肺组织病料,进行病原分离培养和特异性PCR检测。结果从9个鼻拭子和4个肺组织中分离到支原体,经鉴定均为绵羊肺炎支原体,未发现丝状支原体簇成员及多杀性巴氏杆菌和溶血性曼氏杆菌。结果表明,绵羊肺炎支原体是引起该山羊群发生胸膜肺炎的病原,同时说明绵羊肺炎支原体也是山羊支原体性肺炎的重要病原之一。

关键词: 山羊; 绵羊肺炎支原体; 分离; 鉴定

Abstract: A total of 12 nasal swabs and 4 lung specimens were collected from a goat flock with infectious pleuropneumonic disease in Lezhi, Sichuan province. Pathogen isolation and PCR detection were performed for all samples. The results showed that Mycoplasma ovipneumoniae was isolated and identified from 9 nasal swabs and all 4 lung specimens, while any of Mycoplasma mycoides cluster members, Pasteurella multocida or Mannheimia haemolytica was not found. This indicated that Mycoplasma ovipneumoniae is responsible for the pleuropneumonic disease in this flock. It is also suggested that Mycoplasma ovipneumoniae is one of the important pathogens causing Mycoplasma pneumoniae in goats.

Key words: goat; Mycoplasma ovipneumoniae; isolation; identification

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