›› 2010, Vol. 37 ›› Issue (7): 109-112.

• 遗传繁育 • 上一篇    下一篇

绒山羊X染色体7个微卫星标记的遗传连锁图谱的构建

徐磊1,2,姚继广3,杨子森3,赖双英1,张文广1,2,李金泉2,吴萍1,2,王志新1,2,乔峰4,王敏1,2,呼都特1,孟克格日乐1,2   

  1. (1.内蒙古农业大学动物科学与医学学院,呼和浩特 010018; 2.内蒙古自治区动物遗传育种与繁殖重点实验室,呼和浩特 010018; 3.山西省生态畜牧产业管理站,太原 030002; 4.内蒙古白绒山羊种羊场,鄂尔多斯 017000)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-20 发布日期:2010-07-20
  • 通讯作者: 赖双英;李金泉

A Linkage Map of 7 Microsatellite Markers on Chromosome X in Cashmere Goats

XU Lei1,2,YAO Ji-guang3,YANG Zi-sen3,LAI Shuang-ying1,ZHANG Wen-guang1,2,LI Jin-quan2,WU Ping1,2,WANG Zhi-xin1,2,QIAO Feng4,WANG Min1,2,HU Dute1,MENG Kegerile1,2   

  1. (1.Animal Science and Medicine College,Inner Mongolia Agricultural University,Hohhot 010018,China;2.Inner Mongolia Autonomous Region Key Laboratory of Animal Genetics,Breeding and Reproduction,Hohhot 010018,China; 3.Shanxi Ecological Animal Husbandry Industry Management Center,Taiyuan 030002,China;4.Inner Mongolia Cashmere Goat Breeding Farm,Ordos 017000,China)〖
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20
  • Contact: LAI Shuang-ying;LI Jin-quan

摘要: 本研究利用内蒙古白绒山羊的7个父系半同胞家系共794个个体,用X染色体上的7个微卫星标记进行了系谱确认,构建了绒山羊X染色体遗传连锁图。结果表明,7个标记的等位基因数变化范围为9~14,杂合度在0.585~0.918之间,平均杂合度为0.726;多态信息含量在0.759~0.897之间,平均多态信息含量为0.834。构建的内蒙古白绒山羊X染色体遗传连锁图总长度139.4 cM,与英国罗斯林研究所公布的SM4.7绵羊连锁图标记顺序一致,可用于下一步的QTL定位研究。

关键词: 绒山羊; 微卫星标记; X染色体; 连锁图

Abstract: The 7 microsatellite markers on chromosomes X were used to construct a linkage map of Cashmere goats with a 794 members half-sib pedigrees after paternity testing. The results showed that the number of alleles for 7 markers was 9 to 14, the heterozygosity was 0.585 to 0.918, the average heterozygosity was 0.726, and polymorphic information content (PIC) was 0.759 to 0.897, the average polymorphic information content was 0.834. Length of the linkage map of chromosome X we built was 139.4 cM, and the sequence of loci was coincident with the SM4.7, and could be used in quantitative trait loci (QTL) searching in Cashmere goats.

Key words: Cashmere goat; microsatellite markers; chromosomes X; linkage map

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