›› 2010, Vol. 37 ›› Issue (11): 132-135.

• 遗传繁育 • 上一篇    下一篇

绵羊DECR1基因exon 5部分序列多态性分

刘冬1，刘桂林2   

  1. (1.山西农业大学文理学院，太谷 030801；2.山西农业大学动物科技学院，太谷 030801)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-11-20 发布日期:2010-11-20
  • 通讯作者: 刘桂林

Polymorphism Analysis of Exon5 of 2,4-dienoyl-CoA Reductase1 Gene in Sheep

LIU Dong1，LIU Gui-lin2   

  1. (1.College of Arts and Science，Shanxi Agricultural University，Taigu 030801，China；2.College of Animal Science and Veterinary Medicine，Shanxi Agricultural University，Taigu 030801，China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-11-20 Published:2010-11-20

摘要： 试验根据GenBank登录的牛2,4-双烯-CoA还原酶1（2,4-dienoyl-CoA reductase1，DECR1）基因序列（NP_001068891）设计引物，应用PCR技术对德国美利奴绵羊、杜泊绵羊、特克塞尔绵羊DECR1基因的exon 5部分序列进行克隆测序；利用PCR-SSCP技术检测了3个绵羊群体exon 5单链构象多态性（SNP），所获序列与GenBank中其他物种exon 5序列进行了同源性比较，并构建了亲缘关系聚类分析图。结果表明，绵羊DECR1基因exon 5长为137 bp，3个绵羊群体中exon 5均不存在SNPs，各种动物之间DECR1基因exon 5的同源性较高，在81.02%（鸡）～97.08%（牛）之间，其中绵羊和牛同源性最高，达到97.08%；与鸡的同源性最低，为81.02%；聚类分析结果显示，绵羊首先与牛聚为一类，再分别与兔子、狗聚为一类，最后分别与人、猪聚为一类，绵羊与牛的亲缘关系最近，与鸡的亲缘关系最远，与传统分类相一致，说明DECR1基因exon 5在动物进化过程中高度保守。

关键词: 绵羊; DECR1基因; exon 5; 序列; SNP

Abstract: According to exon 5 sequences published by GenBank of 2, 4-dienoyl-CoA reductase 1(DECR1) gene of bovine（NP_001068891）, one pair primer was designed to amplifiy exon 5 of DECR1 gene by using PCR technique for three sheep populations including of German MuttanMerino sheep, Dorper sheep and Texel sheep, the single nucleotide polymorphism of three sheep populations were detected by PCR-SSCP, and compared and analyzed with other species of chicken, rat, sheep, pig, human, bovine, rabbit, dog from GenBank. The results showed that the size of DECR1 gene exon 5 was 137 bp, SNPs was not found in exon 5 of DECR1 gene by PCR-SSCP. By means of sequence homology aligment between sequence of exon 5 of sheep DECR1 gene and others species, the results showed that homology of different species DECR1 gene was 81.02% to -97.08%, the highest similarity was 97.08％ between sheep and bovine. The molecular phylogenetic tree constructed by DECR1 gene exon 5 sequences showed that sheep clustered with bovine firstly，and secondly with rabbit and dog, and then human and pig. This results indicated that sheep had the most nearest relative relationship with bovine, but the far-thest with chicken. It was consistent with the orthodox taxonomic results, and DECR1 gene was very conservative in the progress of animal evolution

Key words: sheep; DECR1 gene; exon5; sequence; SNP

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