›› 2009, Vol. 36 ›› Issue (11): 74-76.

• 生物技术 • 上一篇    下一篇

不同日龄大白猪甲状腺素运载蛋白基因的表达研究

杨国伟,张冬杰,富相奎,何鑫淼,刘娣   

  1. (黑龙江省农业科学院, 哈尔滨 150086)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-11-20 发布日期:2009-11-20
  • 通讯作者: 刘娣

Research on Expression of Large White Pig Transthyretin Gene on Different Days

YANG Guo-wei, ZHANG Dong-jie, FU Xiang-kui, HE Xin-miao, LIU Di   

  1. (Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-11-20 Published:2009-11-20
  • Contact: LIU Di

摘要: 本研究采用RT-PCR方法对大白猪的甲状腺素运载蛋白基因在1、90、180、270和360 d的心、肝、胃、脾、肾、肺、大肠、小肠、肌肉、子宫、卵巢共11个组织的表达情况进行了研究。结果表明,甲状腺素运载蛋白(transthyretin, TTR) mRNA在大白猪的肝脏、子宫和卵巢持续表达,且在肝脏中持续高表达,这与RBP mRNA的表达情况一致;1 d时所检的11个组织均表达,且表达量较高,而90 d时除了心脏、肝脏和胃外,其余组织的表达水平均较低。

关键词: 大白猪; TTR; RT-PCR; 表达

Abstract: Expression level of TTR gene in large white pig heart, liver, stomach, spleen, kidney, lung, large intestine, small intestine, muscle, uterus and ovary on 1, 90, 180, 270 and 360 d were studied by RT-PCR method. The results showed that TTR mRNA was continue expressed in liver, uterus and ovary, and continue higher expressed in liver, it was accord with RBP mRNA. It was higher expressed in eleven tissues on 1 d, but lower in other tissues except heart, liver and stomach on 90 d.

Key words: large white pig; transthyretin; RT-PCR; expression

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