马卵母细胞胞质内精子注射后体外发育能力的研究

›› 2009, Vol. 36 ›› Issue (11): 108-113.

马卵母细胞胞质内精子注射后体外发育能力的研究

王荣祥^1，2，叶小芳^2，3，张国庭²，陈静波²

（1.新疆农业大学动物科学学院，乌鲁木齐 830052； 2.新疆畜牧科学院畜牧科学研究所，乌鲁木齐 830000；3.石河子大学动物科技学院，石河子 832003）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-11-20 发布日期:2009-11-20
通讯作者: 陈静波

Study on the Developmental Competence of Equine Oocytes after ICSI and the Corpus Luteum Ultrastructure during Post-pregnancy Period in Mare

WANG Rong-xiang^1,2， YE Xiao-fang^2,3， ZHANG Guo-ting²，CHEN Jing-bo²

(1.The College of Animal Sciences， Xinjiang Agricultural University， Urumqi 830052， China； 2.Institute of Animal Sciences， Xinjiang Academy of Animal Sciences， Urumqi 830000， China；3.College of Animal Science and Technology, Shihezi University， Shihezi 832003， China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-11-20 Published:2009-11-20
Contact: CHEN Jing-bo

摘要/Abstract

摘要： 本研究在非繁殖季节评估卵丘形态(松散型、致密型)、成熟培养体系(TCM 199、NCSU 23)、体外成熟时间(34、38 h)和离子霉素结合6-DMAP激活对马卵母细胞胞质内精子注射(ICSI)后体外发育能力的影响。从屠宰场采集马卵巢，获得的卵母细胞进行体外成熟，然后注射马冷冻解冻精液，统计分裂情况。试验结果表明，①马松散型卵母细胞成熟率显著高于致密型卵母细胞(P<0.05)，分别为61.09%和41.24%，但ICSI后36 h分裂率无显著差异(P>0.05)，分别为47.34%和44.92%；②两种培养体系对马松散型或致密型卵母细胞成熟率及ICSI后36 h分裂率无显著影响(P>0.05)，但相同成熟体系培养松散型卵母细胞成熟率均显著高于致密型卵母细胞(P<0.05)，然而ICSI后36 h分裂率差异不显著(P>0.05)；③松散型或致密型卵母细胞在TCM 199或NCSU 23中成熟38 h成熟率均高于34 h成熟率，分别为44.43%～68.87%和34.52%～58.90%，松散型卵母细胞在TCM 199体系中成熟34 h、ICSI后激活组或对照组的分裂率显著高于成熟38 h、ICSI后激活组的分裂率(P<0.05)，以及致密型卵母细胞在TCM 199体系中成熟34 h、ICSI后激活组的分裂率(P<0.05)，而且显著高于松散型卵母细胞在NCSU 23体系中成熟38 h、ICSI后对照组的分裂率(P<0.05)；④ICSI后用离子霉素结合6-DMAP激活对马卵母细胞ICSI后36 h分裂无显著影响(P>0.05)。因此，马松散型和致密型卵母细胞的成熟能力存在差异，TCM 199和NCSU 23成熟体系对这2种类型卵母细胞的发育能力无显著影响(P>0.05)，马卵母细胞成熟38 h成熟率高于34 h成熟率，TCM 199成熟体系培养松散型卵母细胞34 h进行ICSI后的分裂率最高。离子霉素结合6-DMAP激活对TCM 199或NCSU 23体系成熟马卵母细胞ICSI后的体外发育能力无显著影响(P>0.05)。

关键词: 马; ICSI; 辅助生殖; 体外受精

Abstract: This study was conducted to evaluate the effect of initial cumulus morphology (expanded or compact)，systems of in vitro maturation (TCM 199 or NCSU 23) and duration of in vitro maturation (34 or 38 h) on the developmental competence of equine oocytes after intracytoplasmic sperm injection (ICSI) in non-breeding season.Oocytes were collected from slaughterhouse-derived ovaries，matured in vitro，and injected with frozen-thawed stallion sperm. The study on the developmental competence of equine oocytes after ICSI indicated that：① The maturation rates of expanded (Ex) oocytes were significantly higher than those of compact (Cp) oocytes (P<0.05，61.09% vs 41.24%).Thirty-six hours after ICSI of mature oocytes，the rate of cleavage with normal morphology was no significant difference with expanded or compact oocytes (P>0.05，47.34% vs 44.92%). ② Two different systems of in vitro maturation did not affect development of embryos from expanded or compact oocytes at 36 h after ICSI (P>0.05).The maturation rates of expanded oocytes were significantly higher than compact oocytes when matured in the same system (P<0.05)，but there is no significant difference for the rate of cleavage with normal morphology at 36 h after ICSI(P>0.05). ③ The maturation rates were higher for oocytes matured for 38 h than for those matured for 34 h (44.43% to 68.87% vs 34.52% to 58.90%). The rate of cleavage with normal morphology at 36 h after ICSI with or without activation were significantly higher for expanded oocytes matured in TCM 199 for 34 h than matured for 38 h with activation (P>0.05)，for compact oocytes matured in TCM 199 for 34 h with activation(P<0.05)，and for expanded oocytes matured in NCSU 23 for 38 h without activation (P<0.05). ④ Yet，combined activation with ionomysin and 6-DMAP did not affect the rate of cleavage with normal morphology at 36 h after ICSI (P>0.05).These data indicate that developmental competence differs between expanded and compact equine oocytes，and is differentially affected by the duration of maturation.

Key words: mare; ICSI; assisted reproductive technology; in vitro fertilization

中图分类号:

Q492.6

王荣祥;叶小芳;张国庭;陈静波. 马卵母细胞胞质内精子注射后体外发育能力的研究[J]. , 2009, 36(11): 108-113.

WANG Rong-xiang;YE Xiao-fang;ZHANG Guo-ting;CHEN Jing-bo. Study on the Developmental Competence of Equine Oocytes after ICSI and the Corpus Luteum Ultrastructure during Post-pregnancy Period in Mare[J]. , 2009, 36(11): 108-113.

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