›› 2008, Vol. 1 ›› Issue (8): 48-51.

• 遗传繁育 • 上一篇    下一篇

西北肉用绵羊新品群陶滩寒组合的微卫星标记研究

杨博辉, 刘建斌, 郎侠, 郭健, 孙晓萍, 程胜利   

  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-08-20 发布日期:2008-08-20

Study on Microsatellite Marker of Hybridization Combination of Poll Dorset, Tan Sheep and SmallTail Han Sheep for New Population of Mutton Sheep in Northwest of China

YANG Bo-hui, LIU Jian-bin, LANG Xia, GUO Jian, SUN Xiao-ping, CHENG Sheng-li   

  • Received:1900-01-01 Revised:1900-01-01 Online:2008-08-20 Published:2008-08-20

摘要: 利用微卫星标记技术,分析了无角陶赛特羊、滩羊、小尾寒羊及其杂交后代滩寒F1、陶滩寒F1 5个绵羊群体的遗传多样性,结果表明,7个微卫星位点均为高度多态位点,平均多态信息含量(PIC)达0.7077~0.8395;5个群体平均位点杂合度达0.7454~0.8665。以Nei氏遗传距离的UPGMA和NJ聚类结果表明,滩羊与滩寒F1具有较近的亲缘关系,小尾寒羊与陶滩寒F1具有较近的亲缘关系,可聚为一类;滩羊、小尾寒羊与陶赛特羊具有较远的亲缘关系。

关键词: 绵羊; 微卫星; 多态信息含量(PIC); 遗传多态性; Nei氏遗传距离

Abstract: The genetic diversity of three sheep breeds and two crossbreeds was surveyed using 7 microsatellite loci. The five breeds include Polled Dorset sheep, Tan sheep, Small Tail Han sheep, the Crossbreed of Tan sheep and Small Tail Han sheep, the Crossbreed of Polled Dorset sheep and the Crossbreed of Tan sheep and Small Tail Han sheep. The 7 microsatellite loci showed high polymorphism information content value. The mean polymorphism information content value was between 0.7077 and 0.8396, which indicated that the 5 breeds had abundant genetic diversity. The mean heterozygosity was between 0.7454 and 0.8665. In the UPGMA dendrogram and NJ based on the Nei genetic distance, Tan sheep and the Crossbreed of Tan sheep and Small Tail Han sheep had the intimate relationship, Small Tail Han sheep and the Crossbreed of Polled Dorset sheep the Crossbreed of Tan sheep and Small Tail sheep had close relationship, which were classified as one sort. But Tan sheep and Small Tail sheep had distant relationship with Polled Dorset sheep.

Key words: sheep; microsatellite; polymorphism information content (PIC); genetic diversity; Nei genetic distance

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