›› 2008, Vol. 1 ›› Issue (7): 76-78.

• 遗传繁育 • 上一篇    下一篇

不同季节水牛卵母细胞体外受精效果的研究

黄芬香,庞春英,张秀芳,覃广胜,陈明棠,杨春艳,李日聪,李婥,梁贤威   

  1. 中国农业科学院水牛研究所,南宁 530001
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-07-20 发布日期:2008-07-20

In Vitro Fertilization and Subsequent Embryo Development Using Oocytes  Derived from Abattoir Ovaries in Different Seasons in Buffalo

HUANG Fen-xiang, PANG Chun-ying,ZHANG Xiu-fang,QIN Guang-sheng,CHEN Ming-tang,YANG Chun-yan,LI Ri-cong,LI Zhuo, LIANG Xian-wei   

  1. Institute of Buffalo Research,Chinese Academy of Agriculture Sciences,Nanning 530001,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-07-20 Published:2008-07-20

摘要: 利用屠宰场收集水牛卵巢回收卵母细胞,比较了不同季节的水牛卵母细胞体外受精的效果。试验连续进行了2年,按水牛发情的规律,1年的试验数据分3个阶段统计。结果表明,水牛发情旺季(8~11月)平均每个水牛卵巢回收可用卵母细胞3.66枚,明显高于发情淡季的4~7月和12月~次年3月两个季节(分别为2.76和3.07枚,P<0.01);而在不同季节回收的水牛卵母细胞体外受精后分裂率和囊胚率差异不显著(P>0.05)。

关键词: 季节; 水牛; 卵母细胞; 体外受精

Abstract: The buffalo oocytes were derived from abattoir ovaries , the effects of IVF of oocytes in different seasons were compared. The experiment has been lasted for two years, the data were divided into tree groups and statistically analyzed according to the oestrum cycle in buffalo. The results indicated that an average of 3.66 usable oocytes were derived from an ovary in oestrum season (Aug-Nov) which was significant higher than those in Mar-Jul and Dec-Feb (2.76 and 3.07 respectively, P<0.01). The cleavage rate and blastocyst rate of IVF oocytes obtained in different season showed no difference(P>0.05).

Key words: season; buffalo; oocyte; IVF

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