›› 2008, Vol. 1 ›› Issue (12): 89-92.

• 遗传繁育 • 上一篇    下一篇

绵羊雌激素受体基因外显子4多态性分析

狄冉1,贾立华2,储明星1,陈宏权2,方丽1   

  1. 1.中国农业科学院北京畜牧兽医研究所,中国农业科学院家养动物遗传资源与种质创新重点开放实验室, 北京 100193;2.安徽农业大学动物科技学院, 合肥 230036
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-12-20 发布日期:2008-12-20

Polymorphism Analysis of Exon 4 of EstrogenReceptor Gene in Sheep

DI Ran1, JIA Lihua2, CHU Mingxing1, CHEN Hongquan2, FANG Li1   

  1. 1. The Key Laboratory of Domestic Animal Genetic Resources and Germplasm Innovation of CAAS, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-12-20 Published:2008-12-20

摘要: 根据GenBank发表的人、鸡、大鼠雌激素受体(estrogen receptor,ESR)基因外显子4的序列设计1对引物,采用PCRSSCP技术分析ESR基因外显子4在高繁殖力绵羊品种(小尾寒羊和湖羊)和低繁殖力绵羊品种(特克塞尔、中国美利奴、考力代和杜泊)中的单核苷酸多态性,同时研究该基因对小尾寒羊高繁殖力的影响。结果表明,ESR基因的此对引物扩增片段在所检测的6个绵羊品种中均不存在PCRSSCP多态性,说明所检测的ESR基因外显子4序列比较保守,该区域可能不是影响绵羊高繁殖力的功能结构域。

关键词: 绵羊; 繁殖力; 雌激素受体基因; PCRSSCP

Abstract: The estrogen receptor (ESR) gene was studied as a candidate gene for the high prolificacy of Small Tail Han sheep. A pair of primers was designed by sequence of exon 4 of ESR gene in human, chicken and rat. Single nucleotide polymorphisms (SNPs) of exon 4 of ESR gene were detected in both high prolificacy breeds (Small Tail Han and Hu sheep) and low prolificacy breeds (Texel, Chinese Merino, Corriedale and Dorper sheep) by polymerase chain reaction (PCR)single strand conformation polymorphism (SSCP). No polymorphism was detected in the amplified region in the six sheep breeds tested. The results indicated that the sequence of exon 4 of ESR gene was rather conserved, and exon 4 may not be the functional domain that affects the high prolificacy in sheep.

Key words: sheep; prolificacy; estrogen receptor gene; PCRSSCP