›› 2007, Vol. 34 ›› Issue (9): 36-39.

• 遗传繁育 • 上一篇    下一篇

影响卵母细胞体外成熟的因素

梁素丽,李向臣,白修云,王有柱,关伟军   

  1. 中国农业科学院北京畜牧兽医研究所,北京 100094
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-09-20 发布日期:2007-09-20

Effects of Factors on IVM of Bovine Oocytes

LIANG Suli,LI Xiangchen,BAI Xiuyun,WANG Youzhu,GUAN Weijun
  

  1. Insitute of Animal Science,Chinese Academy of Agricultural Science,Beijing 100094,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-20 Published:2007-09-20

摘要: 卵母细胞是胚胎工程技术研究中最为重要的一种试验材料,卵母细胞的体外成熟(maturation in vitro,IVM)可以为用体细胞核移植的方法生产大型转基因动物的研究提供大量MⅡ期卵母细胞,同样也是动物体外受精技术的关键环节。作者就卵母细胞体外成熟的影响因素做了简要的论述,主要介绍了卵母细胞来源、其培养系统、激素和生长因子等因素对卵母细胞体外成熟的影响。

关键词: 卵母细胞; 体外成熟; 影响因素

Abstract: Oocytes are one of the most important experiment materials.IVM of oocytes can improve a great deal of MⅡ oocytes for produceing transfer gene animal by somatic cell nuclear transfer.It is the key technical of IVF. This paper briefly introduced the effects of factors on IVM of bovine oocytes.Mainly introduced the factors of oocytes source,culture system,hormone,growth factor and so on.

Key words: oocytes; IVM; factors

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