›› 2007, Vol. 34 ›› Issue (12): 39-41.

• 遗传繁育 • 上一篇    下一篇

江苏省野生及家养中华蜜蜂微卫星DNA遗传多样性分析

吉挺,陈晶,殷灵,包文斌,陈国宏   

  1. 扬州大学动物科学与技术学院,扬州 225009
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-12-20 发布日期:2007-12-20

Study on Microsatellite Genetic Polymorphism of Wild and Domestic Apis Cerana Cerana Populations from Jiangsu Province

JI Ting, CHEN Jing, YIN Ling, BAO Wenbin, CHEN Guohong
  

  1. Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-12-20 Published:2007-12-20

摘要: 利用8对微卫星标记对江苏省野生及家养中华蜜蜂群体遗传多样性进行分析,评估群体内的遗传变异和群体间的遗传分化,结果表明,共检测到31个等位基因,每个位点的等位基因数从1~7不等,平均等位基因数为3.875;所有位点平均的期望杂合度和PIC值分别为0.4424和0.4054。野生及家养中华蜜蜂群体8个微卫星位点平均有效等位基因数分别为3.83和4.17,平均基因杂合度为0.0778和0.0993,两个群体均表现出较低的群体杂合度和遗传多样性。群体分化系数为4.9%(P<0.001),两个群体的Reynolds’遗传距离和Nm值分别为0.05024和4.852。江苏省野生及家养中华蜜蜂群体均表现出较低的群体杂合度和遗传多样性水平,且有
相互交流的迹象。

关键词: 中华蜜蜂; 微卫星; 遗传多样性

Abstract: Genetic diversity of wild and domestic apis cerana cerana populations
from Jiangsu province was evaluated with 8 microstaellite loci. The genetic variability within populations and genetic differentiation between two populations were estimated, the results showed that in 8 microstaellite loci, the 31 alleles were found, the number of alleles per locus ranged from 1 to 7, mean numbers of effective alleles was 3.875, the average expected heterozygosity and PIC of all loci were 0.0993 and 0.0778, respectively. Mean numbers of effective alleles of red jungle fowl in wild and domestic populations were 3.83 and 4.17. Genetic differentiation index (FST) of these two populations was 4.9%(P<0.001), Reynolds’genetic distance and gene flow between the two populations were 0.05024 and 4.852 respectively. These results indicate that the heterozygosity and the genetic
diversity of these two apis cerana cerana populations were rather low, and suggest a tendency towards intermixing.

Key words: apis cerana cerana; microsatellite marker; genetic diversity

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