›› 2007, Vol. 34 ›› Issue (1): 94-96.

• 疾病防治 • 上一篇    下一篇

羊传染性脓疱病毒分离及生物学特性研究

罗云,申之义
  

  1. 内蒙古农业大学动物科学与医学学院,呼和浩特 010018
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-01-20 发布日期:2007-01-20

Isolation and Biological Characterization of Orf Virus

LUO Yun, SHEN Zhiyi   

  1. Animal Science and Veterinary College,Inner Mongolia Agricultural University,Huhhot 010018,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-01-20 Published:2007-01-20

摘要: 用犊牛睾丸细胞及BK细胞,从我国内蒙古自治区达拉特旗发病的进口小尾寒羊痂块中分离到1株病毒,对其进行连续传代至病变稳定。通过对该分离株进行动物回归试验,在犊牛睾丸细胞及BK细胞中的增殖特性试验、病毒核酸类型鉴定试验、脂溶剂敏感试验及其它生物学特性试验、电镜观察等,初步鉴定该分离株为羊传染性脓疱病毒。

关键词: 羊; 传染性脓疱病毒; 生物学特性; 分离

Abstract: From the scab of the sick sheep ,one strain of virus was isolated using Calf Testicle cell and BK cell,then,it was not taken cell passaged until the CPE is order .the recurrent infection,replication test, Aether and Chloroformstability test,and other biological property,electrongraph test proved it was Orf virus.

Key words: sheep; orf virus; biological property; isolate

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