›› 2007, Vol. 34 ›› Issue (1): 39-41.

• 添加剂与饲料 • 上一篇    下一篇

蒽酮-硫酸法测定酵母多糖的含量

王秋菊1, 许丽1, 崔一喆2, 潘军3
  

  1. 1.东北农业大学动物营养研究所,哈尔滨 150030; 2.黑龙江八一农垦大学动物科技学院,大庆163319; 3.哈尔滨市远大牧业有限公司, 哈尔滨 150060
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-01-20 发布日期:2007-01-20

Determination of Yeast Polysaccharide by Anthranone-sulfuric Acid

WANG Qiuju1, XU Li1, CUI Yizhe2, PAN Jun3
  

  1. 1.Department of Animal Sciences, Northeast Agriculture University, Harbin 150030, China;  2.Department of Animal Science, Heilongjiang August First Agriculture University, Daqing 163319, China; 3.Harbin Yuanda Company Limifed, Harbin 150060, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-01-20 Published:2007-01-20

摘要: 用蒽酮-硫酸法测定多糖含量的方法被各种测糖的专著收载,而文献报道的酵母多糖含量的测定一般采用的都是苯酚显色法。作者旨在采用蒽酮硫酸法测定酵母制品多糖的含量,为酵母多糖含量的测定提供一种新方法。结果,摸索出一套适合酵母多糖含量测定的蒽酮硫酸比色法,采用本方法对酵母培养液进行测定,得到了准确的多糖含量,且本方法具有简单、易掌握、快速等优点,对生产上检测多糖含量具有实用价值。

关键词: 酵母; 多糖; 蒽酮硫酸; 分光光度法

Abstract: Determination of polysaccharide by anthranone-sulfuric acid has been recorded by various kinds of books, but it has not been used to estimate the yeast polysaccharide. This text was being to estimate the yeast polysaccharide by anthranone-sulfuric acid, and to grope a new way for the determination of yeast polysaccharide. Exact content of yeast polysaccharide has been gained by anthranone-sulfuric acid. The anthranone-sulfuric acid using to determinate the yeast polysaccharide is a way that is a simple、real time operation and that is easy to palmar grasp. And it has utility value for determination of polusaccharide in produce.

Key words: yeast; polysaccharide; anthranonesulfuric acid; spectrophotography

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