不同生长速度撒坝猪背最长肌lncRNA筛选与功能预测

doi:10.16431/j.cnki.1671-7236.2025.06.004

摘要/Abstract

摘要： 【目的】筛选快、慢生长速度撒坝猪背最长肌组织中的长链非编码RNA(long non-coding RNA,lncRNA)，并探讨其在猪生长发育过程中的重要作用。【方法】选择322日龄体重最大和最小的撒坝猪各6头(公、母各3头)，分为快长组(fast-growth group,FG)和慢长组(slow-growth group,SG)，分别取其背最长肌样品进行转录组测序。测序数据经质控、比对和拼接后，使用DESeq2软件包筛选出快长组和慢长组的差异表达lncRNA，预测其靶基因，并对靶基因进行GO功能和KEGG通路富集分析。随机挑选6个差异表达lncRNAs进行实时荧光定量PCR验证。【结果】共筛选出165个差异表达lncRNAs，其中71个上调，94个下调，并预测到了65个靶基因。GO功能和KEGG通路富集分析结果显示，靶基因显著富集在脂质代谢过程、经典Wnt信号通路等功能条目，以及Wnt信号通路、精氨酸和脯氨酸代谢、组氨酸代谢等通路。实时荧光定量PCR结果显示，所挑选的6个差异表达lncRNAs(ENSSSCT00000085548、MSTRG.8918.1、MSTRG.20943.30、MSTRG.7048.7、MSTRG.11735.9、MSTRG.20943.40)的表达趋势与转录组测序结果一致，且在快、慢长组撒坝猪背最长肌中均存在显著差异(P＜0.05)。【结论】本研究发现的部分lncRNAs与肌肉生长发育过程有关，其可能通过调控WNT10B、SFRP4、LEP、MAOB、ARG1、PRICKLE1和DUSP1等基因的表达影响平均日增重等生长性状，进而导致撒坝猪群体内的生长速度存在差异。研究结果为深入揭示猪生长发育的分子调控机制及平均日增重等生长性状的遗传改良提供了理论依据。

关键词: 撒坝猪; 背最长肌; 转录组测序; lncRNA; 生长发育

Abstract: 【Objective】 This study was aimed to identify the long non-coding RNA (lncRNA) in longissimus dorsi muscle tissue of Saba pigs with fast and slow growth rates,and explore its important roles in pig growth and development.【Method】 Six Saba pigs (three males and three females) with the largest and smallest body weight at 322 days were selected and divided into fast-growth group (FG) and slow-growth group (SG),and the longissimus dorsi muscle samples were collected for RNA-Seq,respectively.After quality control,alignment and splicing of the sequencing data,the DESeq2 software package was used to identify the differentially expressed lncRNA in FG and SG.Target genes were further predicted and analyzed for GO function and KEGG pathway enrichment.Six differentially expressed lncRNAs were randomly selected for Real-time quantitative PCR verification.【Result】 A total of 165 differentially expressed lncRNAs were identified,of which 71 were up-regulated and 94 were down-regulated,and 65 target genes were predicted.The results of GO function and KEGG pathway enrichment analysis showed that the target genes were significantly enriched in functional terms such as lipid metabolism process,canonical Wnt signaling pathway,as well as in pathways including Wnt signaling pathway,arginine and proline metabolism,histidine metabolism,etc.Real-time quantitative PCR results showed that the expression trends of six differentially expressed lncRNAs (ENSSSCT00000085548,MSTRG.8918.1,MSTRG.20943.30,MSTRG.7048.7,MSTRG.11735.9 and MSTRG.20943.40) were consistent with RNA-Seq results,there were significant difference in longissimus dorsi muscle between FG and SG in Saba pigs (P＜0.05).【Conclusion】 lncRNAs identified in this study were related to the process of muscle growth and development,and these lncRNAs could affect growth traits such as average daily weight gain by regulating the expression of genes such as WNT10B,SFRP4,LEP,MAOB,ARG1,PRICKLE1 and DUSP1,which might lead to the differences in growth rate within the population of Saba pigs.The results provided a theoretical basis for further revealing the molecular mechanism of growth and development and the genetic improvement of growth traits such as average daily gain in pigs.

Key words: Saba pigs; longissimus dorsi muscle; RNA-Seq; lncRNA; growth and development

中图分类号:

S813.3
Q78

任灏, 朱怡轩, 晁婷婷, 王孝义, 鲁绍雄, 杨永立, 陈强, 李明丽. 不同生长速度撒坝猪背最长肌lncRNA筛选与功能预测[J]. 中国畜牧兽医, 2025, 52(6): 2494-2505.

REN Hao, ZHU Yixuan, CHAO Tingting, WANG Xiaoyi, LU Shaoxiong, YANG Yongli, CHEN Qiang, LI Mingli. Identification and Functional Prediction of lncRNA in Longissimus Dorsi Muscle of Saba Pigs with Different Growth Rates[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2494-2505.

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