桃金娘水提物抗肝脏纤维化作用的试验研究

doi:10.16431/j.cnki.1671-7236.2021.01.040

摘要/Abstract

摘要： 本试验旨在研究桃金娘水提物对CCl₄复合因素造模法诱导的肝脏纤维化大鼠的保护作用，探讨其作用机制。取SD大鼠72只，随机分为模型组、秋水仙碱组（1×10^-4 mg/g）、桃金娘水提物低剂量（1.5 mg/g）、中剂量（3 mg/g）、高剂量（6 mg/g）组及正常组，每组12只。除正常组外，其余各组大鼠于第1~4周皮下注射40% CCl₄花生油溶液，注射剂量为0.3 mL/100 g体重，隔日1次，连续4周，同时第2~4周给予30%乙醇灌胃1 mL/只，隔日1次，连续3周。各组大鼠于第5周开始灌胃给药，灌胃体积量为1 mL/100 g体重，正常组、模型组给予等量生理盐水，桃金娘水提物组分别按照相应剂量灌胃给药，每日1次，连续给药4周，给药的同时仍继续造模。给药结束后，检测各组大鼠血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）活性、总胆红素（TBil）、α-平滑肌肌动蛋白（α-SMA）、转化生长因子-β（TGF-β）等的水平、丙二醛（MDA）含量及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性；观察肝脏组织HE及Masson染色的变化。试验结果显示，桃金娘水提物可显著或极显著降低肝脏纤维化大鼠AST、ALT活性、TBil、TGF-β及α-SMA水平（P<0.05；P<0.01），提高大鼠血清SOD、GSH-Px活性（P<0.05），降低MDA含量（P<0.01；P<0.05），减轻肝脏纤维化大鼠肝脏病理损伤程度。以上结果表明，桃金娘水提物对CCl₄复合因素所致肝脏纤维化大鼠的肝脏功能具有明显的恢复作用，可增强其抗氧化能力，减轻肝细胞损伤，具有较好的抗肝脏纤维化作用。

关键词: 桃金娘; CCl₄; 肝脏纤维化; 抗氧化

Abstract: This experiment was conducted to study the protective effect of water extract of Rhodomyrtus tomentosa (Ait.) Hass on liver fibrosis rats induced by CCl₄ composited factor,and investigated its mechanism.72 SD mice were randomly divided to normal group,model group,colchicine group,low dose(1.5 mg/g),middle dose (3 mg/g) and high dose (6 mg/g) of water extract of Rhodomyrtus tomentosa (Ait.) Hass,12 mice for each group.Except for the normal group,rats in other groups were subcutaneously injected with 40% CCl₄ peanut oil solution at 1-4 weeks,0.3 mL/100 g body weight,once every other day for 4 weeks.At the same time,30% ethanol was gavaged as 1 mL/piece for 2-4 weeks,once every other day for 3 consecutive weeks.The rats in each group were given gavage at the 5th week,the normal control group and the model group were given normal saline,and the treatment group was given gavage at the corresponding dose,1 mL/100 g body weight,once a day for 4 weeks continuously.The rats in each group were still given gavage at the same time.At the end of the administration,serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity,total bilirubin (TBil) and alpha smooth muscle actin (α-SMA),transforming growth factor β (TGF-β) levels,malondialdehyde (MDA) content and superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) activity were detected.The changes of HE and Masson staining in liver tissues were observed.The results showed that water extract of Rhodomyrtus tomentosa (Ait.) Hass could significantly or extremely significantly reduce the activity/levels of AST,ALT,TBil,TGF-β and α-SMA in rats with liver fibrosis (P<0.05; P<0.01),increase the activity of SOD and GSH-Px in serum (P<0.05),reduce the content of MDA (P<0.05; P<0.01),and reduce the pathological damage degree of liver in rats with liver fibrosis.The above results showed that water extract of Rhodomyrtus tomentosa (Ait.) Hass had an obvious restorative effect on liver function of CCl4 composited factor induced liver fibrosis rats,enhanced its antioxidant capacity,alleviated hepatocyte injury,and had a good effect against liver fibrosis.

Key words: Rhodomyrtus tomentosa (Ait.) Hass; CCl₄; liver fibrosis; antioxidation

中图分类号:

S853.74

梁丽清, 黄鑫, 陈少锋, 杨增艳, 苏华珍, 卢苇, 王河. 桃金娘水提物抗肝脏纤维化作用的试验研究[J]. 中国畜牧兽医, 2021, 48(1): 367-374.

LIANG Liqing, HUANG Xin, CHEN Shaofeng, YANG Zengyan, SU Huazhen, LU Wei, WANG He. Experimental Studies on Anti-liver Fibrosis Effects of Water Extract of Rhodomyrtus tomentosa (Ait.) Hass[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 367-374.

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