中药复方对大肠杆菌感染雏鸭的防治效果研究

doi:10.16431/j.cnki.1671-7236.2020.10.037

摘要/Abstract

摘要： 本研究对由地锦草、石榴皮等组成的中药复方对大肠杆菌感染雏鸭的临床治疗效果进行了研究，旨在为临床大肠杆菌感染提供新的治疗手段。试验选取50只健康1日龄雏鸭，随机分为5组，每组10只。试验组分别为空白对照组、模型组、阿莫西林组、中药复方高剂量组（DS_H）和低剂量组（DS_L）。给药组在3日龄开始给药，连续给药至试验结束，共10 d。除空白对照组外，在雏鸭6日龄时用大肠杆菌腹腔注射进行攻毒，0.5 mL/只。比较各试验组雏鸭的病死率；对病死鸭和各组存活鸭进行解剖，观察肝脏和小肠的病理剖检及HE染色切片；检测血常规及血清生化各项指标，评价中药复方对感染大肠杆菌雏鸭的治疗效果。结果显示，DS_H、DS_L和阿莫西林均能够有效降低感染雏鸭的死亡率；与模型组相比，阿莫西林和DS_H、DS_L组均可减少肝脏、小肠等器官的损伤。与空白对照组相比，模型组的谷草转氨酶（AST）和谷丙转氨酶（ALT）显著升高（P<0.05），碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）和尿素（UREA）极显著升高（P<0.01），且模型组和给药组血液中白细胞（WBC）、红细胞（RBC）、血红蛋白（HGB）、血小板（PLT）等指标变化显著（P<0.05）。与模型组相比，给药组ALP显著或极显著降低（P<0.05；P<0.01），血液中WBC、RBC、HGB、PLT等指标极显著升高（P<0.01），说明给药组机体损伤程度低于模型组。以上结果表明，中药复方可以减轻因大肠杆菌所引起的机体损伤，且作用效果与阿莫西林相近。

关键词: 大肠杆菌; 雏鸭; 体内; 中药; 治疗效果

Abstract: In this study,the clinical therapeutic effect of Chinese herbal compound composed of Euphorbia humifusa Willd.and Punica granatum L.on ducklings infected with Escherichia coli (E.coli) was studied in order to provide a new therapeutic method for clinical treatment of E.coli.Fifty healthy one-day-old ducklings were selected and randomly divided into five groups,ten ducklings in each group.The experimental groups were blank control group,model group,amoxicillin group,Chinese medicine compound high-dose group(DS_H) and low-dose group(DS_L).The administration group started administration at 3 days of age,continued administration for 3 days,and continued administration until the end of the experiment,10 days total.Except for control group,when the ducklings were 6 days old,E. coli was intraperitoneally injected for challenge,0.5 mL/head.The mortality of the experimental groups was compared.The dead ducks and the surviving ducks were dissected,the pathological section of liver and small intestine and HE staining sections were observed.The blood routine indexes and serum biochemical analysis were detected to evaluate the therapeutic effect of traditional Chinese medicine compound on E.coli infected ducklings.The results showed that the high and low doses of the traditional Chinese medicine compound and the amoxicillin could effectively reduce the mortality of infected ducklings.Compared with model group,amoxicillin and traditional Chinese medicine high and low dose groups could reduce liver,small intestine and other organs damage.Compared with control group,aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in model group increased significantly (P<0.05),and alkaline phosphatase (ALP),lactate dehydrogenase (LDH),UREA also showed extremely significant upward trend (P<0.01),and the WBC,RBC,HGB,PLT and other indicators changed extremely significantly(P<0.05).Compared with model group,the alkaline phosphatase(ALP)value was significantly or extremely decreased in the administration group(P<0.05;P<0.01),and the blood WBC,RBC,HGB and PLT indexes were extremely significantly increased (P<0.01).The results indicated that the degree of body damage in the administration group was lower than that in the model group.

Key words: Escherichia coli; ducklings; in vivo; Chinese medicine; treatment effect

中图分类号:

S853.74

秦枫, 刘云, 吴植, 封琦, 吴双, 宋海港, 吴晓洁, 朱善元. 中药复方对大肠杆菌感染雏鸭的防治效果研究[J]. 中国畜牧兽医, 2020, 47(10): 3371-3378.

QIN Feng, LIU Yun, WU Zhi, FENG Qi, WU Shuang, SONG Haigang, WU Xiaojie, ZHU Shanyuan. Study on the Prevention and Control Effect of Chinese Herbal Compound on E.coli Infected Ducklings[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3371-3378.

参考文献 18

[1]	陆承平.兽医微生物[M].第5版.北京:中国农业出版社,2013. LU C P.Veterinary Microorganisms[M].5th Edition.Beijing:China Agriculture Press,2013.(in Chinese)
[2]	王云.鸡大肠杆菌病的防控措施[J].中国畜牧兽医文摘,2016,32(5):155. WANG Y.Prevention and control measures for chicken colibacillosis[J].Chinese Abstracts of Animal Husbandry and Veterinary Medicine,2016,32(5):155.(in Chinese)
[3]	WANG S,NIU C,SHI Z,et al.Effects of ibeA deletion on virulence and biofilm formation of avian pathogenic Escherichia coli[J].Infection and Immunity,2011,79(1):279-287.
[4]	DHO MOULIN M,FAIRBROTHER J M.Avian pathogenic Escherichia coli (APEC)[J].Veterinary Research,1999,30(2-3):299.
[5]	岳秀英,葛荣,吴晓岚,等.四川省猪、鸡源大肠杆菌对抗生素耐药性研究[J].中国兽医杂志,2017,1:93-95. YUE X Y,GE R,WU X L,et al.Study on antibiotic resistance of Escherichia coli from pigs and chickens in Sichuan province[J].Chinese Journal of Veterinary Medicine,2017,1:93-95.(in Chinese)
[6]	宁官保,牛艺儒,张鼎,等.鸡源大肠杆菌耐药性分析及中药对大肠杆菌耐药性消除作用的研究[J].畜牧兽医学报,2015,46(6):1018-1025. NING G B,NIU Y R,ZHANG D,et al.Studies on the drug resistance of chicken isolated Escherichia coli and role of traditional Chinese medicines in the drug resitance elimination[J].Chinese Journal of Animal and Veterinary Sciences,2015,46(6):1018-1025.(in Chinese)
[7]	HALFAOUI Z,MENOUERI N M,BENDALI L M.Serogrouping and antibiotic resistance of Escherichia coli isolated from broiler chicken with colibacillosis in center of Algeria[J].Veterinary World,2017,10(7):830-835.
[8]	KOO H J,WOO G J.Distribution and transferability of tetracycline resistance determinants in Escherichia coli,isolated from meat and meat products[J].International Journal of Food Microbiology,2011,145(2):407-413.
[9]	贺常亮,韦旭斌,付本懂,等.抗鸡大肠杆菌O78单味中药及有效成分的筛选[J].中国畜牧兽医,2013,40(6):178-184. HE C L,WEI X B,FU B D,et al,Screening of single Chinese herbal medicines and effective ingredients for anti-chicken E.coli O78[J].China Animal Husbandry & Veterinary Medicine,2013,40(6):178-184.
[10]	张步彩,袁橙,苏治国,等.4味中药复方治疗鸡大肠杆菌病效果的研究[J].中国畜牧兽医,2019,46(9):2803-2812. ZHANG B C,YUAN C,SU Z G,et al.Study on the effect of 4 traditional Chinese medicine compouds on treating colibacillosis of chicken[J].China Animal Husbandry & Veterinary Medicine,2019,46(9):2803-2812.
[11]	于苗苗,姜晓文,于文会,等.中药复方对雏鸡大肠杆菌病受损脏器治疗效果[J].黑龙江畜牧兽医,2018,3:215-218. YU M M,JIANG X W,YU W H,et al.Therapeutic effect of traditional Chinese medicine compound on damaged organs of chicken colibacillosis[J].Hei-longjiang Animal Science and Veterinary Medicine,2018,3:215-218.(in Chinese)
[12]	江超,姚飞虹,张之怡,等.地锦草与5种抗菌药联用对猪链球菌的体外抑菌效果[J].安徽农业科学,2019,47(24):114-116. JIANG C,YAO F H,ZHANG Z Y,et al.Antibacterial effect of Euphorbia humifusa combining with five antimicrobials against Streptococcus suis in vitro[J].Journal of Anhui Agricultural Sciences,2019,47(24):114-116.(in Chinese)
[13]	贾艳华,李荣誉,张国祖,等.地锦草与10种抗菌药联用对鸡大肠杆菌的体外抑菌效果[J].黑龙江畜牧兽医,2014,7:126-129. JIA Y H,LI R Y,ZHANG G Z,et al.Antibacterial effect of Euphorbia humifusa combining with five antimicrobials against chicken E.coli in vitro[J].Heilongjiang Animal Husbandry and Veterinary Medicine,2014,7:126-129.(in Chinese)
[14]	王红均,程邓芳,先宏,等.17味中草药及其复方对鸽沙门氏杆菌的体外抑菌试验[J].黑龙江畜牧兽医,2019,15:135-137. WANG H J,CHENG D F,XIAN H,et al.In vitro bacteriostatic test of 17 kinds of Chinese herbal medicine and their compound against Salmonella from pigeon[J].Heilongjiang Animal Husbandry and Veterinary Medicine,2019,15:135-137.(in Chinese)
[15]	柯文杰,覃华斌,陈言峰,等.25种中草药对普通变形杆菌的体外抑菌试验[J].水产科技情报,2019,46(6):328-331. KE W J,QIN H B,CHEN Y F,et al.In vitro antibacterial test of 25 Chinese herbal medicines against common Proteus[J].Fishery Science and Technology Information,2019,46(6):328-331.(in Chinese)
[16]	操刚,赵骥,陈红光,等.当归多糖对小鼠试验性肝损伤的影响[J].湖北科技学院学报(医学版),2004,18(5)5:332-334. CAO G,ZHAO J,CHEN H G,et al.Effect of Angelica sinensis polysaccharide on experimental hepatic injury in mice[J].Journal of Hubei University of Science and Technology(Medical Sciences),2004,18(5):332-334.(in Chinese)
[17]	黄诗琦,韦雅妮,赵雨川,等.鸡血藤总黄酮对大肠杆菌败血症的治疗作用[J].中国畜牧兽医,2019,46(5):297-306. HUANG S Q,WEI Y N,ZHAO Y C,et al.The rapeutic effect of total flavonoid of Spatholobus suberectus Dunn on Escherichia coli sepsis[J].China Animal Husbandry & Veterinary Medicine,2019,46(5):297-306.(in Chinese)
[18]	王华,张鲁滨,张雪冰,等.艾叶水提液增强家兔抗大肠杆菌免疫的研究[J].畜牧兽医学报,2019,50(11):2348-2356. WANG H,ZHANG L B,ZHANG X B,et al.Enhancement of rabbbit immunity against E.coli by oral adminstration of water extract of Artemisia argyi Levl.et vant[J].Acta Veterinary et Zootechnica Sinica,2019,50(11):2348-2356.(in Chinese)