响应面法优化微生物除臭效果的研究

doi:10.16431/j.cnki.1671-7236.2020.08.039

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (8): 2684-2693.doi: 10.16431/j.cnki.1671-7236.2020.08.039

• 环境安全 • 上一篇

响应面法优化微生物除臭效果的研究

邵栓^1,2, 党晓伟³, 李慧娟⁴, 常娟¹, 王平¹, 尹清强¹, 高天增⁵

1. 河南农业大学牧医工程学院, 郑州 450002;
2. 新疆玛纳斯县农业农村局, 昌吉 832200;
3. 河南德邻生物制品有限公司, 新乡 453000;
4. 河南省郑州种畜场, 郑州 450011;
5. 河南广安生物科技股份有限公司, 郑州 450001

收稿日期:2019-12-09 出版日期:2020-08-20 发布日期:2020-08-15
通讯作者: 尹清强 E-mail:qqy1964@126.com
作者简介:邵栓(1993-),男,河南周口人,硕士,研究方向:饲料生物技术,E-mail:2683685352@qq.com
基金资助:
河南省重大科技专项（171100110500）；新乡市重大科技专项（ZD19005）

Study on Optimizing the Effectiveness of Microbial Deodorization by Response Surface Methodology

SHAO Shuan^1,2, DANG Xiaowei³, LI Huijuan⁴, CHANG Juan¹, WANG Ping¹, YIN Qingqiang¹, GAO Tianzeng⁵

1. College of Animal Science and Veterinary Medicine, Zhengzhou 450002, China;
2. Manasi Bureau of Agriculture and Rural Affairs, Changji 832200, China;
3. Henan Delin Biological Product Co., Ltd., Xinxiang 453000, China;
4. Zhengzhou Breeding Farm of Henan Province, Zhengzhou 450011, China;
5. Henan Guangan Biotechnology Co., Ltd., Zhengzhou 450001, China

Received:2019-12-09 Online:2020-08-20 Published:2020-08-15

摘要/Abstract

摘要： 为了减少畜禽粪便引发的恶臭气体污染，本研究选用具有除臭功能的贝莱斯芽孢杆菌、枯草芽孢杆菌、产朊假丝酵母和干酪乳杆菌4种微生物菌种作为Box-Behnke设计的4个因素，将4种菌种经活化后接入粪便中，以对应活菌数1×10⁴、1×10⁵和1×10⁶ CFU/g作为Box-Behnke设计的3个编码水平。利用响应面设计构建得到29种复合微生物菌株组合，以其对猪粪中吲哚、氨和硫化氢的去除率作为参考指标，优化得到了4株除臭菌种的最优添加比例。结果表明，当菌液浓度为1×10⁸ CFU/mL的贝莱斯芽孢杆菌、枯草芽孢杆菌、产朊假丝酵母和干酪乳杆菌分别以0.47%、0.05%、0.01%和1.00%的比例加入猪粪便中，堆积发酵7 d后对猪粪便中的吲哚、氨和硫化氢的去除率分别为73.59%、63.60%和70.29%。由此可见，经响应面设计优化后的除臭微生物菌种组合具有良好的除臭效果，对控制畜禽粪便恶臭污染具有较高的应用价值。

关键词: 粪便; 吲哚; 氨; 硫化氢; 微生物发酵; 除臭

Abstract: In order to reduce the odor pollution caused by livestock and poultry manure,four species of microbes with deodorization function such as Bacillus velezensis, Bacillus subtilis,Candida utilis and Lactobacillus casei were selected as 4 factors of experimental design in this study.The corresponding viable counts used in pig manure were 1×10⁴,1×10⁵,and 1×10⁶ CFU/g as the three coding levels of the response surface design.In order to obtain an ideal microbial inoculation proportion for fecal deodorization,4 factors and 3 levels were used to construct 29 combinations of microorganisms in Box-Behnke design for removing indole,NH₃ and H₂S in feces.The results showed that the optimal ratio of Bacillus velezensis,Bacillus subtilis,Candida utilis and Lactobacillus casei obtained by response surface optimization were 0.47%,0.05%,0.01% and 1.00% at the concentration of 1×10⁸ CFU/mL,respectively.After 7 d microbial fermentation,the removal rates of indole,NH₃ and H₂S in pig feces were 73.59%,63.60% and 70.29%,respectively.It could be concluded that an ideal compound microbial preparation for pig fecal innocent treatment had been obtained in this study,which provided the high application value to control the odor pollution caused by livestock and poultry manure.

Key words: manure; indole; ammonia; hydrogen sulfide; microbial fermentation; deodorization

中图分类号:

S811

邵栓, 党晓伟, 李慧娟, 常娟, 王平, 尹清强, 高天增. 响应面法优化微生物除臭效果的研究[J]. 中国畜牧兽医, 2020, 47(8): 2684-2693.

SHAO Shuan, DANG Xiaowei, LI Huijuan, CHANG Juan, WANG Ping, YIN Qingqiang, GAO Tianzeng. Study on Optimizing the Effectiveness of Microbial Deodorization by Response Surface Methodology[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2684-2693.

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响应面法优化微生物除臭效果的研究

Study on Optimizing the Effectiveness of Microbial Deodorization by Response Surface Methodology

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