发酵棉粕对肉仔鸡生长性能、屠宰性能、营养物质表观消化率和脂肪沉积的影响

doi:10.16431/j.cnki.1671-7236.2020.08.006

摘要/Abstract

摘要： 试验旨在研究棉粕经热带假丝酵母菌发酵后得到的发酵棉粕对白羽肉鸡生长性能、屠宰性能、营养物质表观消化率和脂肪沉积的影响。试验选取180羽1日龄科宝白羽肉鸡，随机分成3组，每组6个重复，每个重复10羽鸡。对照组饲喂不加菌发酵棉粕（CSM），两个试验组分别饲喂热带假丝酵母菌发酵棉粕（FCSM）、CSM+热带假丝酵母菌（CT-USCM，活菌量同FCSM）。21日龄试验结束。结果表明：①FCSM组肉鸡末重、平均日增重均显著高于对照组（P<0.05），FCSM和CT-USCM组肉鸡的料重比显著低于对照组（P<0.05）；②CT-USCM组肝重率显著高于对照组（P<0.05），FCSM和CT-USCM组的腹脂率和皮下脂肪厚度显著低于对照组（P<0.05）；③FCSM和CT-USCM组肉鸡干物质和粗蛋白质表观消化率均显著高于对照组（P<0.05），粗脂肪和粗灰分表观消化率在各组间差异不显著（P>0.05）；④FCSM和CT-USCM组肉鸡血糖（Glu）、甘油三酯（TG）和低密度脂蛋白（LDL）浓度均显著低于对照组（P<0.05）；⑤FCSM和CT-USCM组肉鸡肝脏和腹脂中激素敏感酯酶（HSL）活性和生长激素（GH）水平均显著高于对照组（P<0.05），两组之间差异不显著（P>0.05）；FCSM组肝脏和腹脂中乙酰辅酶A羧化酶（ACC）和苹果酸酶（ME）活性均显著低于对照组（P<0.05），而肝脏中脂蛋白酯酶（LPL）活性均显著高于对照组（P<0.05），腹脂中脂肪酸合成酶（ME）活性显著低于其他两组（P<0.05）。综上所述，在日粮中添加FCSM和热带假丝酵母菌均能不同程度地促进肉鸡生长性能、屠宰性能，提高营养物质表观消化率，同时还能通过影响脂肪代谢相关酶活和激素参与肉鸡脂肪合成和分解，降低肉鸡腹脂率和皮下脂肪厚度。

关键词: 热带假丝酵母; 发酵棉粕; 生长性能; 屠宰性能; 表观消化率; 脂肪沉积

Abstract: This study was conducted to investigate the effects of fermented cottonseed meal (fermented by Candida tropicalis) on the growth performance,apparent digestibility,carcass traits,and lipid-related indices in broilers.One hundred and eight one-day-old Cobb broilers were randomly assigned to three groups with six replicates of 10 birds in each.The three diets consisted of a control diet supplemented with fermented cottonseed meal without bacteria (CSM,CON) and the experimental diets with fermented cottonseed meal with Candida tropicali (FCSM) and CSM+Candida tropicali (CT-USCM),respectively.The results showed as follows:①FCSM increased the average daily feed intake compared with CON (P<0.05).FCSM and CT-USCM had lower feed and gain ratio than CON group (P<0.05).②The body weight increased in response to dietary FCSM supplementation (P<0.05),the percentage of liver of CT-USCM group was higher than CON group (P<0.05),additionally,FCSM and CT-USCM supplementation decreased the percentage of abdominal fat and subcutaneous fat thickness (P<0.05).③The dietary nutrient digestibility of dry matter and crude protein significantly increased (P<0.05) with FCSM and CT-USCM supplementation.④Serum concentration of glucose,triglyceride,and low-density lipoprotein cholesterol decreased (P<0.05) in FCSM and CT-USCM fed broilers compared with CON group broilers.⑤The activity of hormone-sensitive esterase and the level of growth hormone in the liver and abdominal fat were higher (P<0.05) in FCSM and CT-USCM groups than CON group.The activity of acetyl CoA carboxylase and malic enzyme in the liver and abdominal fat in FCSM group were lower than CON group (P<0.05).The activity of lipoprotein lipase and fatty acid synthase in liver and abdominal fat in FCSM group were higher than the other two groups,respectively (P<0.05).In conclusion,both FCSM and CT-USCM treatment were beneficial for broilers as they positively affect their growth and digestibility in addition to altering their fat deposition.

Key words: Candida tropicalis; fermented cottonseed meal; growth performance; carcass traits; apparent digestibility; fat deposition

中图分类号:

S816.6

牛俊丽, 魏莲清, 张文举, 聂存喜. 发酵棉粕对肉仔鸡生长性能、屠宰性能、营养物质表观消化率和脂肪沉积的影响[J]. 中国畜牧兽医, 2020, 47(8): 2385-2394.

NIU Junli, WEI Lianqing, ZHANG Wenju, NIE Cunxi. Effects of Fermented Cottonseed Meal on Growth Performance,Apparent Digestibility, Carcass Traits,and Lipid-related Indices in Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2385-2394.

参考文献

[1] PANDIT R J,HINSU A T,PATELN V,et al.Microbial diversity and community composition of caecal microbiota in commercial and indigenous Indian chickens determined using 16s rDNA amplicon sequencing[J].Microbiome,2018,6(1):115.
[2] DUARTE K F,JUNQUEIRA O M,BORGES L L,et al.Performance,carcass traits,and body composition of broilers fed different linseed oil levels between 21 and 56 days of age[J].Brazilian Journal of Poultry Science,2013,15:55-60.
[3] TUMOVÁ E A,TEIMOURI.Fat deposition in the broiler chicken:A review[J].Scientia Agriculturae Bohemica,2010,41(2):121-128.
[4] NIE C X,ZHANG W J,GE W X,et al.Effect of cottonseed meal fermented with yeast on the lipid-related gene expression in broiler chickens[J].Brazilian Journal of Poultry Science,2015,17(spe):57-64.
[5] HAVENSTEIN G B,FERKETM P R,QURESHI A.Growth,livability,and feed conversion of 1957 versus 2001 broilers when fed representative 1957 and 2001 broiler diets[J].Poultry Science,2003,82(10):1500-1508.
[6] NIKOLOVA N,PAVLOVSKI Z,MILOSEVIC N,et al.The quantity of abdominal fat in broiler chicken of different genotypes from fifth to seventh week of age[J].Biotechnololy Animal Husbandry,2007,23:331-338.
[7] NIE C X,ZHANG W J,GE W X,et al.Effects of fermented cottonseed meal on the growth performance,apparent digestibility,carcass traits,and meat composition in Yellow-feathered broilers[J].Turkish Journal of Veterinary and Animal Sciences,2015,39:350-356.
[8] HE T,ZHANG H J,WANG J,et al.Application of low-gossypol cottonseed meal in laying hens' diet[J].Poultry Science,2015,94(10):2456-63.
[9] HENRY M H,BROWN G M P P.Pathology and histopathology of gossypol toxicity in broiler chicks[J].Avian Diseases,2001,45(3):598-604.
[10] ZHANG W J,XU Z R,SUN J Y,et al.Effect of selected fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal[J].Journal of Zhejiang University Science B,2006,7(9):690-695.
[11] JOHNSON E A.Biotechnology of non-Saccharomyces yeasts——The ascomycetes[J].Applied Microbiology & Biotechnology,2013,97(2):503-517.
[12] TANG J W,SUN H,YAO X H,et al.Effects of replacement of soybean meal by fermented cottonseed meal on growth performance,serum biochemical parameters and immune function of Yellow-feathered broilers[J].Asian-Australas Journal of Animal Science,2012,25(3):393-400.
[13] 张晓羊,张文举,王永强,等.嗜酸乳杆菌发酵棉粕对黄羽肉鸡免疫功能和抗氧化性能的影响[J].中国畜牧兽医,2017,44(8):2311-2318. ZHANG X Y,ZHANG W J,WANG Y Q,et al.Effect of cottonseed meal fermented by Lactobacillus acidophilus on immune function and oxidation resistance of Yellow-feathered broilers[J].China Animal Husbandry & Veterinary Medicine,2017,44(8):2311-2318.(in Chinese)
[14] JAZI V,BOLDAJI F,DASTAR B,et al.Effects of fermented cottonseed meal on the growth perfor-mance,gastrointestinal microflora population and small intestinal morphology in broiler chickens[J].British Poultry Science,2017,58(4):402-408.
[15] PARK J A,TIRUPATHI PICHIAH P B,YU J J,et al.Anti-obesity effect of kimchi fermented with Weissella koreensis OK1-6 as starter in high-fat diet-induced obese C57BL/6J mice[J].Journal of Applied Microbiology,2012,113(6):1507-1516.
[16] CHA Y S,KIM S R,YANG J A,et al.Kochujang,fermented soybean-based red pepper paste,decreases visceral fat and improves blood lipid profiles in overweight adults[J].Nutrition & Metabolism,2013,10(1):24-24.
[17] NIU J L,ZHANG J,WEI L Q,et al.Effect of fermented cottonseed meal on the lipid-related indices and serum metabolic profiles in broiler chickens[J].Animals,2019,9(11):1-19.
[18] ZHAO X,GUO Y M,Guo S S,et al.Effects of Clostridium butyricum and Enterococcus faecium on growth performance,lipid metabolism,and cecal microbiota of broiler chickens[J].Appllied Microbiol Biotechnology,2013,97:6477-6488.
[19] ALUWONG T,HASSAN F,DZENDA T,et al.Effect of different levels of supplemental yeast on body weight,thyroid hormone metabolism and lipid profile of broiler chickens[J].Journal of Veterinary Medical Science,2013,75(3):291-298.
[20] KALAVATHY R,ABDULLAH N,JALALUDIN S,at al.Effects of Lactobacillus cultures on growth performance,abdominal fat deposition,serum lipids and weight of organs of broiler chickens[J].British Poultry Science,2003,44(1):139-144.
[21] ZHANG W J,XU Z R,ZHAO S H,et al.Development of a microbial fermentation process for detoxification of gossypol in cottonseed meal[J].Animal Feed Science and Technology,2007,135(1-2):176-186.
[22] BAZIZ H,AIN P A,GERAERT J C,et al.Chronic heat exposure enhances fat deposition and modifies muscle and fat partition in broiler carcasses[J].Poultry Science,1996,75(4):505-513.
[23] ZHAO X L,SIEGEL P B,LIU Y P,et al.Housing system affects broiler characteristics of local Chinese breed reciprocal crosses[J].Poultry Science,2012,91(9):2405-2410.
[24] DE COCASINOVA A,MATEOS G G,GONZALEZALVARADO J M,et al.Comparative study of two analytical procedures for the determination of acid insoluble ash for evaluation of nutrient retention in broilers[J].Spanish Journal of Agricultural Research,2011,9:761-768.
[25] 张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社,2003. ZHANG L Y.Feed Analysis and Quality Test Technology[M].Beijing:China Agricultural University Press,2003.(in Chinese)
[26] 魏莲清,牛俊丽,赵官正,等.添加不同水平的发酵棉粕对科宝肉鸡生长性能、屠宰性能和血清生化指标的影响[J].中国畜牧兽医,2019,46(7):1953-1961. WEI L Q,NIU J L,ZHAO G Z,et al.Effects of different levels of fermented cottonseed meal by Candida tropicalis on growth performance,slaughter performance and biochemical indexes in serum of White-feathered broilers[J].China Animal Husbandry & Veterinary Medicine,2019,46(7):1953-1961.(in Chinese)
[27] 周立强,兰丽燕,徐春生,等.枯草芽孢杆菌对黄羽肉鸡生长性能及营养物质代谢率的影响[J].中国畜牧兽医,2012,39(2):71-74. ZHOU L Q,LAN L Y,XU C S,et al.Effect of Bacillus subtilis on growth performance and nutrition metabolic rate in yellow broilers[J].China Animal Husbandry & Veterinary Medicine,2012,39(2):71-74.(in Chinese)
[28] ZHANG W J,XU Z R,SUN J,et al.A study on the reduction of gossypol levels by mixed culture solid substrate fermentation of cottonseed meal[J].Asian Australasian Journal of Animal Sciences,2006,19(19):1314-1321.
[29] NAGALAKSHMI D,RAO S V R,PANDA A K,et al.Cottonseed meal in poultry diets:A review[J].The Journal of Poultry Science,2007,44(2):119-134.
[30] FENG J,LIU X,XU Z R,et al.The effect of Aspergillus oryzae fermented soybean meal on growth performance,digestibility of dietary components and activities of intestinal enzymes in weaned piglets[J].Animal Feed Science and Technology,2007,134(3-4):295-303.
[31] 和小黑.棉粕源枯草芽孢杆菌发酵饲料消化酶活性的研究[D].石河子:石河子大学,2014. HE X H.A study on digestive enzymes activity of Bacillus subtilis fermented feed from cottonseed meal[D].Shihezi:Shihezi University,2014.(in Chinese)
[32] RAMACHANDRAN S,SINGH S K,LARROCHE C,et al.Oil cakes and their biotechnological applications——A review[J].Bioresourec Technololy,2007,98(10):2000-2009.
[33] ASHAYERIZADEH A,DABIRI N,ASHAYERIZADEH O,et al.Effect of dietary antibiotic,probiotic and prebiotic as growth promoters,on growth performance,carcass characteristics and hematological indices of broiler chickens[J].Pakistan Journal of Biological Sciences,2009,12(1):52-57.
[34] 李璐琳,曹珍,钟珊,等.饮用酵母发酵菌液对矮脚黄肉鸡生长性能、消化酶活性和盲肠菌落结构的影响[J].动物营养学报,2018,30(9):457-466. LI L L,CAO Z,ZHONG S,et al.Effects of drinking Saccharomycetes fermented inoculants on growth performance,digestive enzyme activities and cecal microbiota structure of dwarf Yellow broilers[J].Chinese Journal of Animal Nutrition,2018,30(9):457-466.(in Chinese)
[35] SUN H,TANG J W,YAO X H,et al.Effects of dietary inclusion of fermented cottonseed meal on growth,cecal microbial population,small intestinal morphology,and digestive enzyme activity of broilers[J].Tropical Animal Health and Production,2012,45(4):987-993.
[36] 秦康乐,赵振华,王杏龙.复合益生菌对肉仔鸡生长性能和肠道形态的影响[J].当代畜牧,2017,18:26-28. QIN K L,ZHAO Z H,WANG X L.Effects of compound probiotics on growth performance and intestinal morphology in broilers[J].Contemporary Animal Husbandry,2017,18:26-28.(in Chinses)
[37] 李可,罗建杰,孟昆,等.不同益生菌对肉鸡肠道菌群结构的影响[J].动物营养学报,2015,27(11):3516-3526. LI K,LUO J J,MENG K,et al.Effects of different probiotics on intestinal microbial community structure of broilers[J].Chinese Journal of Animal Nutrition,2015,27(11):3516-3526.(in Chinese)
[38] CHAMBERS M C,BRENDAN M,ROBERT B,et al.A cross-platform toolkit for mass spectrometry and proteomics[J].Nature Biotechnology,2012,30(10):918-920.
[39] 聂存喜.酵母发酵棉粕对肉鸡脂类代谢的影响及其代谢组学研究[D].石河子:石河子大学,2015. NIE C X.Effect of Fermented cottonseed meal with yeast on lipid metabolism of broiler chickens and its metabolomic research[D].Shihezi:Shihezi University,2015.(in Chinese)
[40] ABDELRAHMAN M M.Effects of feeding dry fat and yeast culture on broiler chicken performance[J].Turkish Journal of Veterinary and Animal Sciences,2013,37(1):31-37.
[41] 聂存喜,张文举,闫理东,等.棉籽粕源发酵蛋白质饲料的代谢产物研究[J].动物营养学报,2012,24(8):1602-1609. NIE C X,ZHANG W J,YAN L D,et al.A Study of metabolites of protein feed fermented with cottonseed meal mixed substrates[J].Chinese Journal of Animal Nutrition,2012,24(8):1602-1609.(in Chinese)
[42] 徐少辉,武书庚,张海军,等.L-肉碱生理作用及其机理的研究进展[J].动物营养学报,2011,23(3):357-363. XU S H,WU S G,ZHANG H J,et al.Research advances in physiologucal function and mechanism of action of L-carnitine[J].Chinese Journal of Animal Nutrition,2011,23(3):357-363.(in Chinese)
[43] 赵海云.小肽的吸收机制及营养功能[J].安徽农业科学,2013,41(1):146-148. ZHAO H Y.Adsorption mechanism and nutrition function of small peptides[J].Journal of Anhui Agriculture Science,2013,41(1):146-148.(in Chinese)
[44] OBUN C O,YAHAYA M S,OLAFADEHAN O A,et al.Dietary value of honey and it effects on abdominal fat deposit,blood and serum profile of finisher broiler chicks[J].Journal of Agriculture Forestry and the Social Sciences,2010,6(2).
[45] XU Z,YUMING Z,SHUANGSHUANG G,et al.Effects of Clostridium butyricum and Enterococcus faecium on growth performance,lipid metabolism,and cecal microbiota of broiler chickens[J].Applied Microbiology Biotechnology,2013,97(14):6477-6488.
[46] 邹思湘.动物生物化学[M].北京:中国农业出版社,2009. ZOU S X.Animal biochemistry[M].Beijing:China Agriculture Press,2009.(in Chinese)
[47] AO X,YOO J S,ZHOU T X,et al.Effects of fermented garlic powder supplementation on growth performance,blood profiles and breast meat quality in broilers[J].Livestock Science,2011,141(1):85-89.
[48] KALAVATHY R,ABDULLAH N,JALALUDIN S,et al.Effects of Lactobacillus cultures on growth performance,abdominal fat deposition,serum lipids and weight of organs of broiler chickens[J].British Poultry Science,2003,44(1):139-144.
[49] 李艳.糖皮质激素和热应激对蛋鸡肝脏脂肪代谢影响[D].泰安:山东农业大学,2011. LI Y.Effects of glucocorticoids and heat stress on the liver lipid metabolism of laying hens[D].Tai'an:Shandong Agricultural University,2011.(in Chinese)
[50] 邵明丽,许梓荣.猪脂肪代谢及其调控研究进展[J].饲料博览,2002,10:12-15. SHAO M L,XU Z R.Research progress in pig fat metabolism and its regulation[J].Feed Review,2002,10:12-15.(in Chinese)
[51] 赵旭.丁酸梭菌对肉鸡脂肪代谢的影响及其机理研究[D].北京:中国农业科学院,2014. ZHAO X.Effects of Clostridium butyricum on lipid metabolism of broiler chickens and its underlying mechanism[D].Beijing:Chinese Academy of Agricultural Sciences,2014.(in Chinese)