重庆部分羊场沙门氏菌的分离鉴定、耐药表型分析及耐药基因检测

doi:10.16431/j.cnki.1671-7236.2020.07.032

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (7): 2256-2263.doi: 10.16431/j.cnki.1671-7236.2020.07.032

重庆部分羊场沙门氏菌的分离鉴定、耐药表型分析及耐药基因检测

冯林¹, 王旭东¹, 李九彬², 丁红雷¹, 杨玉姣¹

1. 西南大学动物科技学院, 重庆 400715;
2. 西南大学附属中学, 重庆 400700

收稿日期:2020-03-08 出版日期:2020-07-20 发布日期:2020-07-18
通讯作者: 杨玉姣(1985-),女,博士,讲师,研究方向:兽医微生物学及免疫学,E-mail:yangyujiao@swu.edu.cn E-mail:yangyujiao@swu.edu.cn
作者简介:冯林(1997-),女,云南水富县人,学士,研究方向:细菌耐药性及耐药机制,E-mail:1833929232@qq.com
基金资助:
国家"十三五"重点研发计划项目（2017YFD0501703）；重庆市北碚区应用开发计划项目（2018-28）；中央高校基本科研业务费专项资金（XDJK2020C020）；重庆市技术创新与应用发展专项面上项目（cstc2019jscx-msxmX0402）

Isolation,Identification,Drug-resistant Phenotype Analysis and Drug Resistant Gene Detection of Salmonella at Goat Farms in Chongqing

FENG Lin¹, WANG Xudong¹, LI Jiubin², DING Honglei¹, YANG Yujiao¹

1. College of Animal Science and Technology, Southwest University, Chongqing 400715, China;
2. High School Affiliated to Southwest University, Chongqing 400700, China

Received:2020-03-08 Online:2020-07-20 Published:2020-07-18

摘要/Abstract

摘要： 本研究旨在初步了解重庆市羊源沙门氏菌的耐药性及耐药基因流行情况。在5个山羊养殖场采集185份山羊粪便样品，经选择性增菌和PCR鉴定分离沙门氏菌，确定了其血清型，测定了分离菌对28种抗菌药物的敏感性，并检测了喹诺酮耐药决定区（QRDR）的耐药突变位点和质粒介导的喹诺酮耐药（PMQR）、超广谱β-内酰胺酶（ESBL）基因。共分离到羊源沙门氏菌11株，其中10株为德尔卑沙门氏菌。分离的菌株对氨苄西林、头孢唑啉、头孢氨苄、头孢噻肟、四环素和培氟沙星耐药严重；9株菌表现为多重耐药，对3~7类药物耐药。所有菌株均存在QRDR耐药突变且携带bla_TEM基因；7株菌携带1~5种PMQR基因。本研究分离的羊源沙门氏菌对常用抗菌药物整体耐药较为严重，且广泛存在耐药突变和携带耐药基因。

关键词: 山羊; 沙门氏菌; 药物敏感性; 血清型; 耐药基因

Abstract: The purpose of this research was to acquire the situation of drug resistance and resistance genes prevalence of Salmonella isolated from goats in Chongqing.185 fecal samples were collected from 5 goat farms.Salmonella were isolated by selective enrichment and PCR,and their serotypes were determined.Then,the antimicrobial susceptibility of the isolates to 28 antimicrobial agents was measured.The mutations of quinolone resistance-determining region (QRDR),plasmid-mediated quinolone resistance (PMQR) and extended-spectrum β-lactamases (ESBL) genes were also detected.A total of 11 Salmonella isolates were obtained from goats,and 10 of them were S.Derby.The isolated strains were more resistant to ampicillin,cefazolin,cefalexin,cefotaxime,tetracycline and pefloxacin,and 9 strains showed multi-drug resistant to 3-7 classes of antimicrobial agents.All strains had QRDR resistant mutations and carried bla_TEM gene,whereas,7 strains carried 1-5 PMQR genes.On the whole,Salmonella isolated from goats were resistant to common used antimicrobials.Meanwhile,drug resistant mutations and drug resistant genes were widespread in the isolates.

Key words: goats; Salmonella; antimicrobial susceptibility; serotype; drug resistance gene

中图分类号:

S855.1

冯林, 王旭东, 李九彬, 丁红雷, 杨玉姣. 重庆部分羊场沙门氏菌的分离鉴定、耐药表型分析及耐药基因检测[J]. 中国畜牧兽医, 2020, 47(7): 2256-2263.

FENG Lin, WANG Xudong, LI Jiubin, DING Honglei, YANG Yujiao. Isolation,Identification,Drug-resistant Phenotype Analysis and Drug Resistant Gene Detection of Salmonella at Goat Farms in Chongqing[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2256-2263.

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重庆部分羊场沙门氏菌的分离鉴定、耐药表型分析及耐药基因检测

Isolation,Identification,Drug-resistant Phenotype Analysis and Drug Resistant Gene Detection of Salmonella at Goat Farms in Chongqing

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