黄牛呼吸道CD3+淋巴细胞分布的研究

doi:10.16431/j.cnki.1671-7236.2018.05.033

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (5): 1389-1394.doi: 10.16431/j.cnki.1671-7236.2018.05.033

黄牛呼吸道CD3⁺淋巴细胞分布的研究

李乙江¹, 蚌德新², 郭兆廷³, 余绍荣⁴, 丁宏¹, 闫信权¹, 张书光¹, 杨科¹, 郭兆卫¹, 罗祥波¹, 李佳赟¹, 杨倩¹

1. 南京农业大学动物医学院, 南京 210095;
2. 德宏州动物疫病预防控制中心, 德宏 678400;
3. 风平镇农业综合服务中心, 德宏 678400;
4. 陇川县动物疫病预防控制中心, 德宏 678700

收稿日期:2017-10-10 出版日期:2018-05-20 发布日期:2018-05-19
通讯作者: 杨倩 E-mail:zxbyq@njau.edu.cn
作者简介:李乙江(1985-),女,云南瑞丽人,博士,兽医师,研究方向:动物黏膜免疫和分子免疫学,E-mail:429003509@qq.com
基金资助:
国家自然科学基金项目《一种高效提高新生仔猪肠道免疫力的新机制》（31372465）

Study on Distribution of CD3⁺ Lymphocytes in Cattle Respiratory Tract

LI Yijiang¹, BENG Dexin², GUO Zhaoting³, YU Shaorong⁴, DING Hong¹, YAN Xinquan¹, ZHANG Shuguang¹, YANG Ke¹, GUO Zhaowei¹, LUO Xiangbo¹, LI Jiayun¹, YANG Qian¹

1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
2. Dehong Animal Disease Control Center, Dehong 678400, China;
3. Agricultural Comprehensive Service Center of Fengping Town, Dehong 678400, China;
4. Longchuan County Animal Disease Control Center, Dehong 678700, China

Received:2017-10-10 Online:2018-05-20 Published:2018-05-19

摘要/Abstract

摘要：

CD3⁺是T细胞群的重要表面标志，呼吸道黏膜下分布的CD3⁺淋巴细胞作为抗感染黏膜免疫的基础，在保护机体抵抗呼吸道感染中发挥重要作用。为了解黄牛呼吸道CD3⁺淋巴细胞和淋巴组织的分布，本研究运用HE染色法和免疫组织学方法对5头7岁健康婆罗门黄牛的鼻黏膜、气管、肺内支气管及肺脏组织的CD3⁺淋巴细胞和淋巴组织的分布进行了研究。结果显示，黄牛的鼻黏膜、气管、肺内支气管和肺脏组织中均分布有CD3⁺淋巴细胞，且主要分布于黏膜上皮间及其下方固有层中及腺体周围；在鼻黏膜和肺脏组织中分布有CD3⁺淋巴细胞形成的弥散淋巴组织。牛呼吸道中CD3⁺淋巴细胞数量在鼻黏膜和肺脏组织中分布最多，气管黏膜、肺内支气管黏膜次之。本试验结果明确了CD3⁺淋巴细胞在黄牛呼吸道中的分布谱，表明黄牛呼吸道具备引发局部黏膜免疫的基础条件，为牛呼吸道黏膜免疫及呼吸道疾病防治研究提供了数据支撑。

关键词: 黄牛; CD3⁺淋巴细胞; 淋巴组织; 呼吸道组织

Abstract:

CD3⁺ is an important surface marker of T cell population.CD3⁺ lymphocytes which are distributed under respiratory tract mucosae as the basis of anti-infective mucosal immunity play an important role in protecting the body against respiratory infection.In order to understand the distribution profile of CD3⁺ lymphocytes and lymphoid tissues in cattle respiratory tract for the mucosal immune strategy of respiratory tract,the samples of nasal mucosa,trachea,pulmonary bronchia and lung from five 7-month-old health cattle were collected and the distribution of CD3⁺ lymphocytes and lymphoid tissues was tested by HE staining and immunohistochemistry methods.The results showed that CD3⁺ lymphocytes distributed in mucosal epithelium,below lamina propria and around gland in nose,trachea and pulmonary bronchia and lung.CD3⁺ lymphocytes form diffuse lymphoid tissue in nasal mucosa and lung.The number of the CD3⁺ lymphocytes were the largest in nasal mucosa and lung,followed by trachea and pulmonary bronchia.The results made clear the distribution profile of CD3⁺ lymphocytes in cattle respiratory tract.It indicated that the respiratory tract of cattle had the basic conditions for initiating local mucosal immunity.This study would provide the basic data for mucosal immunity in respiratory tract and prevention and treatment for respiratory disease in cattle.

Key words: cattle; CD3⁺ lymphocytes; lymphoid tissue; respiratory tract tissues

中图分类号:

S852.31

李乙江, 蚌德新, 郭兆廷, 余绍荣, 丁宏, 闫信权, 张书光, 杨科, 郭兆卫, 罗祥波, 李佳赟, 杨倩. 黄牛呼吸道CD3⁺淋巴细胞分布的研究[J]. 《中国畜牧兽医》, 2018, 45(5): 1389-1394.

LI Yijiang, BENG Dexin, GUO Zhaoting, YU Shaorong, DING Hong, YAN Xinquan, ZHANG Shuguang, YANG Ke, GUO Zhaowei, LUO Xiangbo, LI Jiayun, YANG Qian. Study on Distribution of CD3⁺ Lymphocytes in Cattle Respiratory Tract[J]. , 2018, 45(5): 1389-1394.

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黄牛呼吸道CD3⁺淋巴细胞分布的研究

Study on Distribution of CD3⁺ Lymphocytes in Cattle Respiratory Tract

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黄牛呼吸道CD3+淋巴细胞分布的研究

Study on Distribution of CD3+ Lymphocytes in Cattle Respiratory Tract

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黄牛呼吸道CD3⁺淋巴细胞分布的研究

Study on Distribution of CD3⁺ Lymphocytes in Cattle Respiratory Tract