地塞米松对仔猪肠道黏膜形态的影响

doi:10.16431/j.cnki.1671-7236.2017.12.039

摘要/Abstract

摘要：

试验旨在研究地塞米松对仔猪肠道黏膜形态的影响，并初步探讨地塞米松对肠道免疫屏障功能的影响以及利用地塞米松建立仔猪肠道损伤模型的可行性，以期为今后研究其他糖皮质类激素引起的肠道损伤发生机制和防治措施提供理论和科学依据。试验选用断奶1周的健康仔猪30头，随机分为6组，每组5个重复，试验Ⅰ、Ⅱ、Ⅲ组分别注射地塞米松1、2、3 ml，对照Ⅰ、Ⅱ、Ⅲ组分别注射生理盐水1、2、3 mL；采用HE染色、PAS染色和ELISA检测法分别测定肠道绒毛高度、隐窝深度、绒毛高度与隐窝深度比值、杯状细胞、上皮内淋巴细胞和肠黏膜分泌型免疫球蛋白A（sIgA）6个指标。试验结果表明，地塞米松能抑制仔猪肠绒毛生长，使隐窝深度变深，肠绒毛高度与隐窝深度比值降低，杯状细胞、上皮内淋巴细胞数目和sIgA含量均减少，且剂量越大以上变化越明显。综合上述结果，地塞米松会影响肠绒毛的发育和肠道营养物质的吸收，破坏黏膜免疫屏障功能，且有剂量依赖性。

关键词: 地塞米松; 仔猪; 肠道黏膜; 形态

Abstract:

This study was aimed to investigate the effects of dexamethasone on the morphology of intestinal mucosa and intestinal immune barrier function, and study the feasibility of setting up the piglets intestinal damage model, which could provide a theoretical and scientific basis for the future research of the mechanism and prevention measures caused by other intestinal injury of glucocorticoids. 30 healthy piglets (1 week after weaning) were randomly divided into 6 groups, with 5 replicates in each group. The experimental groups Ⅰ, Ⅱ and Ⅲ were injected with dexamethasone 1, 2 and 3 mL, the control groups Ⅰ, Ⅱ and Ⅲ were injected with saline 1, 2 and 3 mL,respectively. HE staining,PAS staining and ELISA detection method were used to measure the intestinal villus length and crypt depth, villus length and crypt depth ratio, goblet cells and intraepithelial lymphocytes and sIgA. The results were as follows:Dexamethasone inhibited the growth of intestinal villus, the crypt depth became deeper, villus length and crypt depth ratio decreased,and the number of goblet cells, intraepithelial lymphocytes and sIgA levels were reduced in experimental groups, and the greater the dose, the more obvious the indicators changed. It suggested that dexamethasone could affect the growth of intestinal villus and the intestinal absorption of nutrients, damage mucosal immune barrier function in a dose dependent.

Key words: dexamethason; piglets; intestinal mucosa; morphologye

中图分类号:

S852.16⁺2

潘书磊, 邓红玉, 阮国荣, 王全溪. 地塞米松对仔猪肠道黏膜形态的影响[J]. 《中国畜牧兽医》, 2017, 44(12): 3670-3675.

PAN Shu-lei, DENG Hong-yu, RUAN Guo-rong, WANG Quan-xi. Effect of Dexamethasone on Mucosa Morphology in Piglets[J]. , 2017, 44(12): 3670-3675.

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