新疆伊犁鹅繁殖周期生殖激素变化及卵巢卵泡发育规律的研究

doi:10.16431/j.cnki.1671-7236.2017.11.022

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (11): 3264-3269.doi: 10.16431/j.cnki.1671-7236.2017.11.022

新疆伊犁鹅繁殖周期生殖激素变化及卵巢卵泡发育规律的研究

程元¹, 赵晓钰¹, 李园园¹, 赵全庄², 朱建平³, 艾山江³, 李海英¹

1. 新疆农业大学动物科学学院, 乌鲁木齐 830052;
2. 额敏县恒鑫实业有限公司, 塔城 834600;
3. 塔城地区畜牧科技推广中心, 塔城 834700

收稿日期:2017-04-16 出版日期:2017-11-20 发布日期:2017-11-21
通讯作者: 李海英 E-mail:lhy-3@163.com
作者简介:程元(1991-),女,重庆人,硕士生,研究方向:动物营养与饲料科学,E-mail:463870089@qq.com
基金资助:
农业部物种资源保护项目（13162130135252042）；新疆农业大学研究生科研创新项目（XJAUGRI2016002）

Study on the Changes of Reproductive Hormone and Regulation of Follicle Development During the Reproductive Cycle of Yili Goose in Xinjiang

CHENG Yuan¹, ZHAO Xiao-yu¹, LI Yuan-yuan¹, ZHAO Quan-zhuang², ZHU Jian-ping³, AI Shan-jiang³, LI Hai-ying¹

1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
2. Emin County Hengxin Industrial Co., Ltd., Tacheng 834600, China;
3. Tacheng Regional Animal Husbandry Science and Technology Promotion Center, Tacheng 834700, China

Received:2017-04-16 Online:2017-11-20 Published:2017-11-21

摘要/Abstract

摘要：

为探究新疆伊犁鹅繁殖周期中血浆主要生殖激素、卵巢形态及卵泡发育的变化规律，试验以健康状况良好、体重相近的2岁伊犁鹅为试验对象，分别在产蛋期、就巢期及休产期各随机选取32只，采集1次血样，测定激素水平；在各时期分别选取5只采集卵巢组织，观测卵巢及卵泡发育情况。结果表明，在产蛋期，伊犁鹅的GnRH分别极显著高于就巢期和休产期16.78%和58.29%（P<0.01）；PRL显著低于就巢期13.00%（P<0.05），极显著高于休产期28.94%（P<0.01）；FSH高于就巢期6.98%（P>0.05），极显著高于休产期21.12%（P<0.01）；LH极显著低于就巢期8.38%（P<0.01），极显著高于休产期16.84%（P<0.01）；E₂水平分别极显著高于就巢期和休产期29.80%和112.40%（P<0.01）；P₄水平分别极显著高于就巢期和休产期28.89%和30.34%（P<0.01）。产蛋期伊犁鹅的卵巢体积和重量均极显著大于就巢期和休产期（P<0.01）。产蛋期伊犁鹅成熟卵泡与就巢期和休产期相比明显较大，次级卵泡数量较多，初级卵泡数量较少；就巢期卵巢上生长及成熟卵泡发生闭锁，颗粒胞层收缩，向内凹陷，胞质出现空腔，各级卵泡出现萎缩；与就巢期相比，休产期卵巢上卵泡胞质空腔变大，向内凹陷程度进一步加深，大量原始卵泡均匀排列。由此可见，在产蛋期，伊犁鹅的GnRH、E₂、LH反应活跃，对卵巢卵泡发育起主导作用；在就巢期，PRL、LH维持较高的分泌水平，协同维持就巢。

关键词: 新疆伊犁鹅; 繁殖周期; 生殖激素; 卵泡; 发育规律

Abstract:

This experiment was aimed to study the changes of reproductive hormone and regulation of follicle development during the reproductive cycle of Yili goose in Xinjiang. 2 years old Yili goose were chosen which were in good health and similar weight. 32 samples were randomly selected to take blood samples for hormone detection during the laying, broodiness and ceased periods, and 15 samples were randomly selected to gather ovarian tissue for the observation of the development of ovary and follicular. The results showed that during the laying period, the GnRH of the Yili goose was extremely significantly higher than the broodiness and ceased periods of 16.78% and 58.29% (P<0.01);PRL was significantly lower than the broodiness period of 13.00% (P<0.05), and was extremely significantly higher than the ceased period of 28.94% (P<0.01); FSH was higher than the broodiness period of 6.98% (P>0.05),and was extremely significantly higher than the ceased period of 21.12% (P<0.01); LH was extremely significantly lower than the broodiness period of 8.38% (P<0.01),and was extremely significantly higher than the ceased period of 16.84% (P<0.01); E₂ were extremely significantly higher than that the broodiness and ceased periods of 29.80% and 112.40% (P<0.01); P₄ levels were extremely significantly higher than the broodiness and ceased periods of 28.89% and 30.34% (P<0.01). The ovary volume and weight of Yili goose were extremely significantly higher than that of broodiness and ceased periods (P<0.01). In the laying period, the mature follicles of Yili goose were significantly larger than that of the broodiness and resting periods, the number of secondary follicles was more and the number of primary follicles was lesser. Ovarian growth and mature follicle atresia, granule layer shrinkage, inward depression, cytoplasmic cavity, follicular atrophy at all levels in the broodiness period. The follicle cytoplasmic cavity of the ovary was larger in the ceased period than broodiness period, and inward concave further deeper, a large number of primordial follicles evenly arranged. Thus, during the laying period, the responses of GnRH, E₂ and LH of Yili goose were active and played a leading role on the development of ovarian and follicular. PRL and LH maintained a high level of secretion, cooperated to maintain the broodiness behavior during the broodiness period.

Key words: Yili goose; breeding cycle; reproductive hormones; follicles; growth rule

中图分类号:

S835

程元, 赵晓钰, 李园园, 赵全庄, 朱建平, 艾山江, 李海英. 新疆伊犁鹅繁殖周期生殖激素变化及卵巢卵泡发育规律的研究[J]. 《中国畜牧兽医》, 2017, 44(11): 3264-3269.

CHENG Yuan, ZHAO Xiao-yu, LI Yuan-yuan, ZHAO Quan-zhuang, ZHU Jian-ping, AI Shan-jiang, LI Hai-ying. Study on the Changes of Reproductive Hormone and Regulation of Follicle Development During the Reproductive Cycle of Yili Goose in Xinjiang[J]. , 2017, 44(11): 3264-3269.

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新疆伊犁鹅繁殖周期生殖激素变化及卵巢卵泡发育规律的研究

Study on the Changes of Reproductive Hormone and Regulation of Follicle Development During the Reproductive Cycle of Yili Goose in Xinjiang

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