罗伊氏乳杆菌抑制致病性大肠杆菌感染效果的研究

doi:10.16431/j.cnki.1671-7236.2017.08.030

摘要/Abstract

摘要：

本研究旨在探索益生罗伊氏乳杆菌抑制致病性大肠杆菌感染昆明鼠的效果、剂量及潜在机制。将96只昆明鼠随机分为4个组，每组4个重复，每个重复6只，分别为对照组、低剂量组、中剂量组和高剂量组。昆明鼠饲喂商品化基础日粮，对照组饮用自来水，低、中、高剂量组在饮水中分别添加1.0×10⁶、1.0×10⁷及1.0×10⁸ CFU/mL罗伊氏乳杆菌。试验期为28 d，试验结束后称重，计算昆明鼠平均日采食量（ADFI）、平均日增重（ADG）及料重比（F/G）；并灌胃致病性大肠杆菌（4.98×10⁹ CFU/mL），观察小鼠死亡率；检测血清大肠杆菌肠毒素含量、抗氧化能力及肠道菌群数量。结果显示，中剂量组小鼠末重和平均日增重均显著高于对照组（P<0.05），小鼠死亡率、血清大肠杆菌肠毒素浓度和丙二醛（MDA）水平均显著低于对照组（P<0.05）。中剂量组血清总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-Px）和总抗氧化力（T-AOC）活力均显著高于对照组（P<0.05），总乳杆菌和双歧杆菌数量显著提高（P<0.05），大肠杆菌和沙门氏菌含量显著降低（P<0.05）。综上，在小鼠饮水中添加罗伊氏乳杆菌可提高生长性能，改善攻毒后机体抗氧化力和盲肠菌群结构，降低血清大肠杆菌肠毒素含量和小鼠死亡率，有效降低致病性大肠杆菌的感染造成的死亡率，其中添加1.0×10⁷ CFU/mL伊氏乳杆菌效果最好。

关键词: 罗伊氏乳杆菌; 昆明鼠; 致病性大肠杆菌; 抗氧化力

Abstract:

This study was aimed to explore the effect,dose of Lactobacillus reuteri on inhibition of pathogenic Escherichia coli infection on Kunming mice as well as its potential mechanism. 96 Kunming mice were randomly allocated into four groups with 4 replicates per group and 6 mice per replicate,which were control group,low dose group,middle dose group and high dose group. All of the mice feed commercial basal feedstuff,meanwhile the mice in control group drank tap-water,three treatments were supplemented with 1.0×10⁶,1.0×10⁷ and 1.0×10⁸ CFU/mL Lactobacillus reuteri in water. The feeding period was 28 days. All the mice were weighted, and the ADFI,ADG and F/G were calculated at the end of experiment.At the day of 28,pathogenic Escherichia coli (4.98×10⁹ CFU/mL) were irrigated to study the mortality. The indexes of intestinotoxin and antioxidation in serum and cecal microbacteria were inspected.The results indicated that supplemented with middle dose of Lactobacillus reuteri could significantly improve the final body weight,ADG compared with the control (P<0.05). The mortality, serum intestinotoxin level and MDA concentration,Escherichia coli and Staphylococcus were significantly lower than control group (P<0.05),while the T-SOD,T-AOC,GSH-Px,Lacobacillus and Bifidobacterium were significantly improved (P<0.05).In conclusion,with Lactobacillus reuteri supplementation,the growth performance was improved and antioxidant capacity and cecal microbacteria were enhanced after pathogenic Escherichia coli infected, mouse mortality caused by the pathogenic Escherichia coli was effectively reduced,and the effect of 1.0×10⁷ CFU/mL Lactobacillus reuteri was the best.

Key words: Lactobacillus reuteri; Kunming mice; pathogenic Escherichia coli; antioxidant capacity

中图分类号:

S816.7

杨家军, 钱坤, 李庆岗, 吴义景, 吴东, 王重龙. 罗伊氏乳杆菌抑制致病性大肠杆菌感染效果的研究[J]. 《中国畜牧兽医》, 2017, 44(8): 2431-2436.

YANG Jia-jun, QIAN Kun, LI Qing-gang, WU Yi-jing, WU Dong, WANG Chong-long. Effects of Lactobacillus reuteri Supplementation on Inhibition of Pathogenic Escherichia coli in Kunming Mice[J]. , 2017, 44(8): 2431-2436.

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