仔猪腹泻致病性大肠杆菌分型鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2018.10.025

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (10): 2876-2884.doi: 10.16431/j.cnki.1671-7236.2018.10.025

仔猪腹泻致病性大肠杆菌分型鉴定及耐药性分析

刘丽娟¹, 马光强², 张胜沾³, 王伟¹, 王开功¹, 周碧君¹, 苟万里⁴

1. 贵州大学动物科学学院, 贵阳 550025;
2. 黔南民族职业技术学院, 都匀 558000;
3. 大方县农牧局动物疫病预防控制中心, 大方 551600;
4. 贵阳学院, 贵阳 550005

收稿日期:2018-03-20 出版日期:2018-10-20 发布日期:2018-10-20
通讯作者: 周碧君, 苟万里 E-mail:bjzhou@gzu.edu.cn;wankege@163.com
作者简介:刘丽娟(1991-),女,贵州松桃人,硕士生,研究方向:兽医公共卫生学,E-mail:1003075708@qq.com
基金资助:
贵州省科学技术厅农业攻关项目"贵州省仔猪腹泻性疫病风险分析及综合防控技术研究"黔科合NY[2015]3009-1号；"贵州省动物疫病防控与兽医公共卫生保障科技创新人才团队"（黔科合人才团队[2015]4016号）；贵州省农业委员会科技计划项"贵州省2015年重大动物疫病省级定点监测"[2015-01号]

Classification and Drug Resistance Analysis of Pathogenic Escherichia coli in Diarrhea Piglets

LIU Lijuan¹, MA Guangqiang², ZHANG Shengzhan³, WANG Wei¹, WANG Kaigong¹, ZHOU Bijun¹, GOU Wanli⁴

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Qiannan National Vocational and Technical College, Duyun 558000, China;
3. Animal Disease Control and Prevention Center, Dafang County Agriculture and Animal Husbandry Bureau, Dafang 551600, China;
4. Guiyang University, Guiyang 550005, China

Received:2018-03-20 Online:2018-10-20 Published:2018-10-20

摘要/Abstract

摘要：

为了解贵州省规模化养猪场腹泻仔猪致病性大肠杆菌流行情况及耐药性变化，本研究运用凝集试验、PCR和药敏纸片琼脂扩散法等方法对分离的78株致病性大肠杆菌进行血清型、毒力基因及耐药性分析。结果显示，78株致病性大肠杆菌以O138、O87血清型为主，占定型菌株的60.8%；其中62株致病性大肠杆菌检出毒力基因，检出率为79.5%，可分为8种毒力基因类型，分属肠致病性大肠杆菌（EPEC）、肠产毒性大肠杆菌（ETEC）和肠聚集性大肠杆菌（EAEC），毒力基因eaeA、elt和escV检出率较高，分别为38.5%、28.2%和21.8%；分离到的致病性大肠杆菌对β-内酰胺类药物高度耐药，均为多重耐药株，耐药种类可达8种以上。结果表明，当前贵州省规模化养猪场腹泻仔猪致病性大肠杆菌的毒力基因检出率较高且基因型复杂，耐药性严重。本试验结果可为规模化养猪场防控仔猪腹泻提供基础资料及理论依据。

关键词: 仔猪腹泻; 致病性大肠杆菌; 血清型; 毒力基因; 耐药性

Abstract:

To understand the epidemic situation and drug resistance of pathogenic Escherichia coli (E.coli) in diarrhea piglets from large-scale swine farms in Guizhou province,agglutination test,PCR and drug sensitive agar diffusion methods were used to perform serotyping and virulence gene classification and drug resistance analysis of 78 pathogenic E.coli isolates in this experiment.The results showed that the main serotypes of 78 pathogenic E.coli were O138 and O87,accounting for 60.8% of the established strains;The virulence genes were detected in 62 strains of pathogenic E.coli,the detection rate was 79.5%,and could be divided into 8 virulence gene types,belonging to enteropathogenic E.coli (EPEC),enterotoxigenic E.coli (ETEC) and enterohemorrhagic E.coli (EAEC),respectively.The virulence genes eaeA,elt and escV had higher detection rate,which were 38.5%,28.2% and 21.8%,respectively.The isolated pathogenic E.coli was highly resistant to β-lactam drugs,and was multi-drug resistant,with more than 8 drug-resistant species.The results showed that the pathogenicity of E.coli in piglets with diarrhea in large-scale pig farms in Guizhou province was high,and the genotypes were complex.The pathogenicity of E.coli was severe.This experiment could provide basic data and theoretical basis for prevention and control of piglets diarrhea in large-scale pig farms.

Key words: piglet diarrhea; pathogenic E.coli; serotype; virulence genes; drug resistance

中图分类号:

S852.61

刘丽娟, 马光强, 张胜沾, 王伟, 王开功, 周碧君, 苟万里. 仔猪腹泻致病性大肠杆菌分型鉴定及耐药性分析[J]. 《中国畜牧兽医》, 2018, 45(10): 2876-2884.

LIU Lijuan, MA Guangqiang, ZHANG Shengzhan, WANG Wei, WANG Kaigong, ZHOU Bijun, GOU Wanli. Classification and Drug Resistance Analysis of Pathogenic Escherichia coli in Diarrhea Piglets[J]. , 2018, 45(10): 2876-2884.

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仔猪腹泻致病性大肠杆菌分型鉴定及耐药性分析

Classification and Drug Resistance Analysis of Pathogenic Escherichia coli in Diarrhea Piglets

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