上海地区屠宰场猪红斑丹毒丝菌的分离鉴定

doi:10.16431/j.cnki.1671-7236.2017.01.032

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (1): 227-235.doi: 10.16431/j.cnki.1671-7236.2017.01.032

上海地区屠宰场猪红斑丹毒丝菌的分离鉴定

徐锋, 王晓旭, 张维谊, 宁昆, 齐新永, 沈莉萍

上海市动物疫病预防控制中心, 上海 201103

收稿日期:2016-04-21 出版日期:2017-01-20 发布日期:2017-01-19
通讯作者: 沈莉萍 E-mail:slplujia@163.com
作者简介:徐锋(1979-),男,上海人,学士,兽医师,研究方向:动物疾病的临床诊断和细菌学检测,E-mail:13321992151@163.com
基金资助:
《上海地区猪红斑丹毒丝菌的分离鉴定及毒力基因的检测》[沪农青字（2015）第2-7号；《上海地区主要动物病原微生物种质资源保存利用》[沪农科攻字]（2014）第7-3-3号]

Isolation and Identification of Erysipelothrix rhusiopathiae from Abattoirs in Shanghai Area

XU Feng, WANG Xiao-xu, ZHANG Wei-yi, NING Kun, QI Xin-yong, SHEN Li-ping

Shanghai Municipal Animal Disease Control Center, Shanghai 201103, China

Received:2016-04-21 Online:2017-01-20 Published:2017-01-19

摘要/Abstract

摘要：

为了解上海地区猪红斑丹毒丝菌在健康猪群中的带菌状况，本试验选择上海地区标准化屠宰场的上市猪为研究对象，采集鼻拭子、肺脏、扁桃体和下颌淋巴结等样本，进行猪红斑丹毒丝菌的分离，采用VITEK 2 Compact系统和VITEK MS快速鉴定系统进行鉴定，并设计1对16S rRNA基因引物，对分离菌株进行PCR扩增，随机选取16株分离菌进行测序及16S rRNA同源性分析。结果显示，49.07%（105/214）的样本中分离到猪红斑丹毒丝菌，分离菌株同参考菌株及疫苗株之间16S rRNA同源性为99.6%~100.0%。本试验结果表明上海地区猪群中确实存在猪红斑丹毒丝菌的健康带菌状况。

关键词: 屠宰场; 猪红斑丹毒丝菌; 分离鉴定

Abstract:

In order to comprehend the status of Erysipelothrix rhusiopathiae carried in swinery in Shanghai area, samples of pigs were collected from some standard abattoirs in Shanghai area, including nasal swab, lung, tonsil and mandibular lymph nodes. Strains were isolated from these samples for detecting the Erysipelothrix rhusiopathiae. Several kinds of methods were used, such as VITEK 2 Compact system and VITEK MS system. A pair of primers was designed to amplify the 16S rRNA gene. 16 PCR products selected randomly were sequenced and 16S rRNA homology analysis was conducted. As a result, strains were isolated from 49.07% (105/214) samples and were identified as Erysipelothrix rhusiopathiae.16S rRNA homology in strains of isolation and reference were 99.6% to 100.0%. The result indicated that the situation was very severe for so many carriers of Erysipelothrix rhusiopathiae in health group in nursery.

Key words: abattoirs; Erysipelothrix rhusiopathiae; isolation and identification

中图分类号:

S858.28

徐锋, 王晓旭, 张维谊, 宁昆, 齐新永, 沈莉萍. 上海地区屠宰场猪红斑丹毒丝菌的分离鉴定[J]. 《中国畜牧兽医》, 2017, 44(1): 227-235.

XU Feng, WANG Xiao-xu, ZHANG Wei-yi, NING Kun, QI Xin-yong, SHEN Li-ping. Isolation and Identification of Erysipelothrix rhusiopathiae from Abattoirs in Shanghai Area[J]. , 2017, 44(1): 227-235.

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上海地区屠宰场猪红斑丹毒丝菌的分离鉴定

Isolation and Identification of Erysipelothrix rhusiopathiae from Abattoirs in Shanghai Area

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