牦牛源牛链球菌的分离鉴定及部分生物学特性研究

doi:10.16431/j.cnki.1671-7236.2016.12.034

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (12): 3314-3321.doi: 10.16431/j.cnki.1671-7236.2016.12.034

牦牛源牛链球菌的分离鉴定及部分生物学特性研究

张兴民, 张焕容, 汤承, 诸明欣

西南民族大学生命科学与技术学院, 成都 610041

收稿日期:2016-04-15 出版日期:2016-12-20 发布日期:2016-12-22
通讯作者: 张焕容 E-mail:zhrong05@163.com
作者简介:张兴民(1990-),男,甘肃张掖人,硕士,研究方向:畜禽传染病诊断与防制,E-mail:2197345890@qq.com
基金资助:
西南民族大学研究生创新项目（CX2016SZ092）；国家民委科技项目（14XNZ025）

Isolation, Identification and Biological Characteristics Research of Streptococcus bovis from Yak

ZHANG Xing-min, ZHANG Huan-rong, TANG Cheng, ZHU Ming-xin

College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China

Received:2016-04-15 Online:2016-12-20 Published:2016-12-22

摘要/Abstract

摘要：

本研究旨在从腹泻牦牛粪便中分离鉴定牛链球菌，并分析其溶血性、对小鼠的致病性及对抗菌药物的敏感性。将川西北阿坝州45份腹泻牦牛粪便于血平板上划线，37℃、5% CO₂培养24 h分离细菌，经16S rRNA序列扩增测序和系统发育分析鉴定出14株牛链球菌，其中8株巴黎链球菌，6株解没食子酸链球菌巴氏亚种；9株呈α溶血，5株呈β溶血。分离鉴定的牦牛源巴黎链球菌和解没食子酸链球菌巴氏亚种能引起试验小鼠的轻度腹泻；药敏试验结果显示分离菌株对青霉素、头孢噻肟、万古霉素、乙酰螺旋霉素、环丙沙星、利福平、替考拉宁、氨苄西林和庆大霉素共9种抗生素高度敏感，对链霉素、卡那霉素、林可霉素、红霉素、四环素和克林霉素耐药率较高。本研究阐明了牦牛源链球菌的部分生物学特性，为该病的防控奠定了基础。

关键词: 牦牛; 牛链球菌; 分离鉴定; 耐药性

Abstract:

The objective of this study was to isolate and identify Streptococcus bovis from yak, and detect their hemolytic, pathogenicity and antimicrobial susceptibility. 45 fecal samples collected from the diarrheal yaks in Aba state in Northwest Sichuan province were used to isolate Streptococcus bovis with sheep blood culture media under 37℃ and 5% CO₂ condition cultured for 24 h. The isolated bacteria were identified by 16S rRNA amplification and sequencing. Total 14 Streptococcus bovis were identified, among which, 8 Streptococcus lutetiensis strains and 6 Streptococcus gallolyticus strains. 9 Streptococcus bovis strains showed α hemolytic, and 5 Streptococcus bovis showed β hemolytic. Both Streptococcus lutetiensis and Streptococcus gallolyticus caused the experimental mice mild diarrhea. The isolated 14 Streptococcus bovis were sensitive to penicillin, cefotaxime, vacomycin, acetylspiramycin, ciprofloxacin, rifampicin, teicoplanin, ampicillin and gentamicin, and highly tolerant to streptomycin, kanamycin, lincomycin, erythrocin, tetracycline and clindamycin.This study illustrated the biological characteristics of the isolated Streptococcus bovis from yak, which made the basis for the prevention and control of diseases caused by Streptococcus bovis.

Key words: yak; Streptococcus bovis; isolation and identification; antimicrobial susceptibility

中图分类号:

S823.8⁺5

张兴民, 张焕容, 汤承, 诸明欣. 牦牛源牛链球菌的分离鉴定及部分生物学特性研究[J]. 《中国畜牧兽医》, 2016, 43(12): 3314-3321.

ZHANG Xing-min, ZHANG Huan-rong, TANG Cheng, ZHU Ming-xin. Isolation, Identification and Biological Characteristics Research of Streptococcus bovis from Yak[J]. , 2016, 43(12): 3314-3321.

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牦牛源牛链球菌的分离鉴定及部分生物学特性研究

Isolation, Identification and Biological Characteristics Research of Streptococcus bovis from Yak

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